Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi

Year 2022, Volume: 6 Issue: 1, 7 - 16, 28.02.2022

Emine Müge Karakayalı , Tuna Önal , Zeynep Gülden Sönmez Tamer Mehmet İbrahim Tuğlu

https://doi.org/10.33716/bmedj.1038292

Abstract

Amaç: Probiyotik (PB) ve Mezenkimal Kök Hücre (MKH) yeni tedavi fikirleridir. PB ile değiştirilen mikrobiyata farklı etkiler yapmaktadır. Son zamanlarda önemli araştırma konusu olmuştur. MKH hücresel tedavi kapsamında MKH Graft Versus Host (GVH) gibi hastalıklarda klinik uygulamada yerini almaktadır. Bu çalışmada kültür ortamında MKH davranışına çoğalma, göç etme, farklanma ve yara iyileşmesi açılarından PB uygulamasının yapacağı etkiler araştırıldı.Gereç ve Yöntem: Dondurulmuş yağ dokusu kökenli MKH (YDMKH) hücre kültüre alınarak oniki kuyucuklu kültür tabağında 2.5x104hücre/cm2 olacak şekilde üretildi. Çoğalması MTT ile, göç etmesi çizik oluşturulması sonrası yara yerinin kapanması ile, farklanması kemik hücresi osteoblasta dönüştürülmesi ile incelendi. PB olarak NBL Probiotic Gold. Preparatı içersindeki 2.5x109 Enterococcus faecium, 2.5x109 lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum bakterileri eklenip aynı ortamdaki MKH için davranış parametreleri açısından incelendi. PB ve MKH ilişkisi taramalı elektron mikroskobi ile de araştırıldı. Bulgular: PB eklenmesinin çoğalmayı hızlandırdığı, göç etmeyi arttırarak yara iyileşmesini çabuklaştırdığı ve osteoblastik kemiksi yapıya farklanmada olumlu katkısının oluştuğu saptandı. İnce yapı incelemesinde PB ile etkileşmiş MKH için daha canlı, daha çok tutunan ve daha aktif hücre morfolojisinde olduğu bulundu. Sonuç: Alınan sonuçlar kültür ortamına PB eklenmesinin MKH etkinliğini arttırdığını gösterdi. Deney hayvanı ile yapılacak çalışmalarda aynı olumlu etkinin görülmesinin klinik için yararlı olacağı düşünüldü.

Keywords

probiyotik, mezenkimal kök hücre, Kültür, Davranış, Farklanma

Supporting Institution

İZMİR DEMOKRASİ ÜNİVERSİTESİ BAP KOMİSYONU

Project Number

TPF-20H05

Thanks

Bu Çalışmayı Destekleyen BAP Komisyonuna ve Üniversitemize teşekkürederim

Effect of probiotics on mesenchymal stem cell behavior in culture

Abstract

Aim: Probiotic (PB) and Mesenchymal Stem Cell (MSC) are new treatment ideas for diseases. Alterations of microbiata made by PB produce different effects. MSC as the scope of cellular therapy takes place in clinical practice in many diseases such as Graft Versus Host (GVD) within the scope of cellular therapy. In this study, the effects of PB application on MSC behavior in culture in terms of proliferation, migration, differentiation and wound healing were investigated. Material and Method: Frozen adipose tissue-derived (AD) MSC cells were cultured and produced at 2.5x104 cells/cm2 in a twelve-well culture dish. They were examined for proliferation by MTT, migration by the closure of the wound after the creation of a scratch, and differentiation by transforming the bone cell osteoblast. NBL Probiotic Gold as PB was used as bacteria which include 2.5x109 Enterococcus faecium, 2.5x109lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum and examined in terms of behavioral parameters for MSC in the same environment. The relationship between PB and MSC was also investigated by Scanning Electron Microscopy (SEM). Result: It was determined that use of PB caused an increase in proliferation, faster wound closure by increase of migration and higher differentiation to osteoblast. The ultrastructural examination of MSC by SEM showed that MSCs interacted with PB are more spread, more adherent, and have more active cell morphology. Conclusion: The results showed that the addition of PB to the culture medium increased the MSC efficiency. It was thought that PB would be more beneficial for the clinic to see of MSC if the same positive effect results in studies with experimental animals.

Keywords

Probiyotic, mesenchymal stem cell, culture, behaviour, differentiation, differentiationdifferentiation

Project Number

TPF-20H05

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