MEZENTERİK İSKEMİ-REPERFÜZYONLA İNDÜKLENEN İNTESTİNAL DOKU HASARINA KARŞI EVODİAMİN’İN ETKİSİ: OKSİDATİF STRESİN ROLÜ The Effect of Evodiamine Against Intestinal Tissue Injury Induced By Mesenteric Ischemia-Reperfusion: Role of Oxidative Stress

Abstract

ÖZET

Amaç: Bu çalışma, evodiaminin mezenterik iskemi-reperfüzyonun neden olduğu intestinal dokudaki

oksidatif hasara karşı muhtemel yararlı etkisini göstermeyi amaçlamıştır.

Materyal ve Metod: Bu amaçla çalışmamızda deney hayvanlarında mezenterik iskemi-reperfüzyon

modeli yapıldı. İskemi-reperfüzyon ve evodiamin düşük ve yüksek doz tedavi gruplarında,

superior mezenterik arter 1 saat süreyle klemplendi. Daha sonra klemp açıldı ve 2 saatlik reperfüzyon

başlatıldı. Deney sürecinin sonlandırılmasından sonra, tüm hayvanlar sakrifiye edildi ve

intestinal doku örnekleri toplandı.

Bulgular: TOS, OSI, MDA düzeyleri ve MPO aktivitesinin sham grubuna göre mezenterik iskemireperfüzyon

grubunda arttığı görüldü. ''Ayrıca mezenterik iskemi-reperfüzyon grubunda SOD aktivitesi

ve TAS seviyesinin sham grubuna göre azaldığı tespit edildi. ''Düşük ve yüksek dozlarda

evodiamin (10 ve 20 mg / kg) uygulanan gruplarda mezenterik iskemi-reperfüzyon grubuna göre

TAS değeri ve SOD aktivitesi artarken, TOS, OSI değerleri, MPO aktivitesi ve MDA düzeyi azaldığı

belirlendi.

Sonuç: İki farklı evodiamin dozu mezenterik iskemi-reperfüzyonun neden olduğu intestinal dokunun

oksidatif hasarına karşı yararlı etkiler göstermiştir.

Anahtar Sözcükler: Mezenterik iskemi-reperfüzyon; Evodiamin; İntestinal doku hasarı; Sıçan

ABSTRACT

Purpose: This study aimed to show possible benefical effect of evodiamine against oxidative

damage of intestinal tissue induced by mesenteric ischemia-reperfusion.

Materials and Methods: For this purpose, in our study mesenteric ischemia-reperfusion model

was conducted in experimental animals. In low and high doses treatment of evodiamine and

ischemia-reperfusion groups, superior mesenteric artery was clamped for 1 h. Then, the clamp

was opened and reperfusion was started for 2 h. After the termination of the experimental

prosses, all animals were sacrificed and intestinal tissue samples were collected.

Results: It was seen that TOS, OSI, MDA levels and MPO activity increased in mesenteric ischemiareperfusion

group compared to sham group. Moreover, SOD activity and TAS level reduced in

mesenteric ischemia-reperfusion group compared to sham group. When compared to mesenteric

ischemia-reperfusion group, TAS value and SOD activity increased while TOS, OSI values, MPO

activity and MDA level decreased in groups treated with low and high doses of evodiamine (10

and 20 mg/kg).

Conclusion: Two different doses of evodiamine revealed benefical effects against oxidative

damage of intestinal tissue induced by mesenteric ischemia-reperfusion.

Keywords: Mesenteric ischemia-reperfusion; Evodiamine; Intestinal tissue damage; Rat

Keywords

• MEZENTERİK İSKEMİ-REPERFÜZYONLA İNDÜKLENEN İNTESTİNAL DOKU HASARINA KARŞI EVODİAMİN’İN ETKİSİ: OKSİDATİF STRESİN ROLÜ The Effect of Evodiamine Against Intestinal Tissue Injury Induced By Mesenteric Ischemia-Reperfusion: Role of Oxidative Stress

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