Is There Intestinal Mucosal Damage in Calves Exposed to Polypharmacy?

Year 2025, Volume: 6 Issue: 1, 22 - 25, 30.06.2025

Deniz Alıç Ural

https://doi.org/10.58833/bozokvetsci.1646856

Abstract

This field study was planned retrospectively and was carried out on 59 calves belonging to different farms within the provincial or district borders of Aydın. Each calf included in the study was selected among those who had been subjected to treatment with more than one drug due to diarrhea (Group I; n=20) and/or pneumonia (Group II; n=20). As a starting criterion, those who had been on multiple drug treatment for at least one week were included. 19 other healthy calves were included in the study as the control group (III) for comparison. Serum was extracted from blood samples obtained from each calf by expert veterinarians after centrifugation and stored in the freezer. Serum DAO values were determined by competitive enzyme immunoassay technique using commercial Bovine Diamine Oxidase (DAO) ELISA tests. The mean±standard error DAO (ng/mL) values were determined as 8,22 ± 2,02, 11,44 ± 1,96 and 28,90 ± 3,59 (p< 0.001) in Groups I, II and III, respectively. As a result, it can be said that intestinal mucosal damage may develop in calves with diarrhea and/or pneumonia exposed to polypharmacy for at least 1 week, and this can be detected by decreased DAO levels compared to healthy controls.

Keywords

calf , diamine oxidase , intestinal mucosal damage , polypharmacy

Polifarmaziye Maruz Kalan Buzağılarda İntestinal Mukozal Hasarlanma Mevcut Mu?

Abstract

Bu saha araştırması retrospektif olarak planlanarak, Aydın’da il ya da ilçe sınırlarında farklı işletmelere ait 59 buzağıda gerçekleştirilmiştir. Araştırma kapsamında yer alan her buzağı, ishal (Grup I; n=20) ve/veya pnömoni (GrupII; n=20) nedeniyle birden fazla ilaç ile sağaltım denemesine tabi tutulmuş olanlar arasından seçildi. Başlangıç kriteri olarak en az bir haftadır çoklu ilaç uygulamasında bulunulanlar dahil edildi. Karşılaştırmalı olarak sağlıklı 19 diğer buzağı da kontrol grubu (III) olarak çalışmada yer buldu. Her buzağıda uzman veteriner hekim eli ile elde edilen kan örneklerinden santrifüj sonrası serum çıkartılarak dondurucuda muhafaza edildi. Ticari Bovine Diamin Oksidaz (DAO) ELISA testleri aracılığında rekabetçi enzim immünoassay tekniği vasıtasıyla serum DAO değerleri saptandı. Sırası ile Grup I, II ve III’te ortalama±standart hata DAO (ng/mL) değerleri 8,22 ± 2,02, 11,44 ± 1,96 ve 28,90 ± 3,59 (p< 0.001) olarak tespit edildi. Sonuç olarak polifarmaziye en az 1 hafta maruz kalan ishalli ve/veya pnömonili buzağılarda intestinal mukozal hasarlanma gelişebileceği, bunun da sağlıklı kontrollere göre azalan DAO seviyeleri ile tespit edilebileceği söylenebilinir.

Keywords

buzağı , diamin oksidaz , intestinal mukozal hasar , polifarmazi

References

• Payne RA. The epidemiology of polypharmacy. Clinical Medicine 2016; 16: 465–469. doi: 10.7861/clinmedicine.16-5-465.
• Hunter RP, Isaza R. Polypharmacy in zoological medicine. Pharmaceutics 2017; 9(1): 10. doi: 10.3390/pharmaceutics9010010.
• Kovacova-Hanuskova E, Buday T, Gavliakova S, Plevkova J. Histamine, histamine intoxication and intolerance. Allergologia et Immunopathologia 2015; 43(5): 498–506. doi: 10.1016/j.aller.2015.05.001.
• Fukudome I, Kobayashi M, Dabanaka K, Maeda H, Okamoto K et al. Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats. Medical Molecular Morphology 2014; 47: 100–107. doi: 10.1007/s00795-013-0060-0.
• Honzawa Y, Nakase H, Matsuura M, Chiba T. Clinical significance of serum diamine oxidase activity in inflammatory bowel disease: importance of evaluation of small intestinal permeability. Inflammatory Bowel Diseases 2011; 17(2): E23–E25. doi: 10.1002/ibd.21401.
• Zhang L, Zhang L, Zhan XA, Zeng X, Zhou L et al. Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88. Journal of Animal Science and Biotechnology 2016; 7(1): 3. doi: 10.1186/s40104-015-0038-9.
• Alizadeh A, Akbari P, Garssen J, Fink-Gremmels J, Braber S. Epithelial integrity, junctional complexes, and biomarkers associated with intestinal functions. Tissue Barriers 2022; 10(3): 1996830. doi: 10.1080/21688370.2021.1996830.
• Song Z, Cheng K, Zhang L, Wang T. Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers. Journal of Thermal Biology 2017; 69: 184–190. doi: 10.1016/j.jtherbio.2017.07.003.
• Alıç Ural D. Investigation of diamine oxidase as a biomarker of intestinal mucosal injury in calves exposed to antibacterial treatment. Journal of Advances in VetBio Science and Techniques 2024; 9(3): 190–196.
• Shakir KM, Margolis S, Baylin SB. Localization of histaminase (diamine oxidase) in rat small intestinal mucosa: site of release by heparin. Biochemical Pharmacology 1977; 26: 2343–2347.
• Ayuso P, Garcia-Martin E, Martinez C, Agundez JA. Genetic variability of human diamine oxidase: occurrence of three nonsynonymous polymorphisms and study of their effect on serum enzyme activity. Pharmacogenetics and Genomics 2007; 17: 687–693.
• Palacios NL, Agundez JA, Diaz-Rubio M, Ayuso P. Analysis of a non-synonymous single nucleotide polymorphism of the human diamine oxidase gene (ref. SNP ID: rs1049793) in patients with Crohn’s disease. Scandinavian Journal of Gastroenterology 2009; 44(9): 1041–1045. doi: 10.1080/00365520903075897. Fukuda T, Tsukano K, Nakatsuji H, Suzuki K. Plasma diamine oxidase activity decline with diarrhea severity in calves indicating systemic dysfunction related to intestinal mucosal damage. Research in Veterinary Science 2019; 126: 127–130. doi: 10.1016/j.rvsc.2019.08.004.
• Quanz S. Dietary interventions to modulate gut function in ruminants. Doctoral dissertation, 2022.
• He L, Wang C, Simujide H, Aricha H, Zhang J et al. Effect of early pathogenic Escherichia coli infection on the intestinal barrier and immune function in newborn calves. Frontiers in Cellular and Infection Microbiology 2022; 12: 818276. doi: 10.3389/fcimb.2022.818276.
• Alıç Ural D. Intestinal mucosal damage and intestinal permeability in non-infectious and infectious diarrheic calves in relation to diamine oxidase activity. International Journal of Veterinary and Animal Research 2024; 7(3): 83–86.
• Alıç Ural D, Erdoḡan S, Kılıç N, Erdoḡan H, Turk E et al. Probiotic enema protects intestinal mucosae and decreases plasma diamine oxidase activity among calves with diarrhea. Veterinaria 2023; 72(3): 283–289.
• Maintz L, Novak N. Histamine and histamine intolerance. American Journal of Clinical Nutrition 2007; 85(5): 1185–1196. doi: 10.1093/ajcn/85.5.1185.