Is There Intestinal Mucosal Damage in Calves Exposed to Polypharmacy?

Yıl 2025, Cilt: 6 Sayı: 1, 22 - 25, 30.06.2025

Deniz Alıç Ural

https://doi.org/10.58833/bozokvetsci.1646856

Öz

This field study was planned retrospectively and was carried out on 59 calves belonging to different farms within the provincial or district borders of Aydın. Each calf included in the study was selected among those who had been subjected to treatment with more than one drug due to diarrhea (Group I; n=20) and/or pneumonia (Group II; n=20). As a starting criterion, those who had been on multiple drug treatment for at least one week were included. 19 other healthy calves were included in the study as the control group (III) for comparison. Serum was extracted from blood samples obtained from each calf by expert veterinarians after centrifugation and stored in the freezer. Serum DAO values were determined by competitive enzyme immunoassay technique using commercial Bovine Diamine Oxidase (DAO) ELISA tests. The mean±standard error DAO (ng/mL) values were determined as 8,22 ± 2,02, 11,44 ± 1,96 and 28,90 ± 3,59 (p< 0.001) in Groups I, II and III, respectively. As a result, it can be said that intestinal mucosal damage may develop in calves with diarrhea and/or pneumonia exposed to polypharmacy for at least 1 week, and this can be detected by decreased DAO levels compared to healthy controls.

Anahtar Kelimeler

calf , diamine oxidase , intestinal mucosal damage , polypharmacy

Polifarmaziye Maruz Kalan Buzağılarda İntestinal Mukozal Hasarlanma Mevcut Mu?

Öz

Bu saha araştırması retrospektif olarak planlanarak, Aydın’da il ya da ilçe sınırlarında farklı işletmelere ait 59 buzağıda gerçekleştirilmiştir. Araştırma kapsamında yer alan her buzağı, ishal (Grup I; n=20) ve/veya pnömoni (GrupII; n=20) nedeniyle birden fazla ilaç ile sağaltım denemesine tabi tutulmuş olanlar arasından seçildi. Başlangıç kriteri olarak en az bir haftadır çoklu ilaç uygulamasında bulunulanlar dahil edildi. Karşılaştırmalı olarak sağlıklı 19 diğer buzağı da kontrol grubu (III) olarak çalışmada yer buldu. Her buzağıda uzman veteriner hekim eli ile elde edilen kan örneklerinden santrifüj sonrası serum çıkartılarak dondurucuda muhafaza edildi. Ticari Bovine Diamin Oksidaz (DAO) ELISA testleri aracılığında rekabetçi enzim immünoassay tekniği vasıtasıyla serum DAO değerleri saptandı. Sırası ile Grup I, II ve III’te ortalama±standart hata DAO (ng/mL) değerleri 8,22 ± 2,02, 11,44 ± 1,96 ve 28,90 ± 3,59 (p< 0.001) olarak tespit edildi. Sonuç olarak polifarmaziye en az 1 hafta maruz kalan ishalli ve/veya pnömonili buzağılarda intestinal mukozal hasarlanma gelişebileceği, bunun da sağlıklı kontrollere göre azalan DAO seviyeleri ile tespit edilebileceği söylenebilinir.

Anahtar Kelimeler

buzağı , diamin oksidaz , intestinal mukozal hasar , polifarmazi

Kaynakça

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