Mavi Yengeçlerden Vibrio parahaemolyticus’un İzolasyonu, İdentifikasyonu ve Bazı Antibiyotiklere Karşı Direnç Profillerinin Belirlenmesi

Year 2022, Volume: 3 Issue: 1, 1 - 4, 30.06.2022

Sadık Büyükyörük Meltem Çalışkan Cemil Sahıner

Abstract

Bu çalışmanın amacı Aydın İli ve çevresinden temin edilen mavi yengeçlerden Vibrio parahaemolyticus’un izolasyonu, identifikasyonu ile bu suşların antibakteriyel direnç profillerinin belirlenmesidir. İzole edilen altmış adet şüpheli izolattan 4’ü, real-time PCR ile Vibrio parahaemolyticus olarak identifiye edilmiştir. Bu suşların, Penicillin G, clindamycin, piperacilin, amoxicillin-clavulanic acid, ciprofloxacin ve gentamicin gibi antibiyotiklere karşı dirençlilikleri disk difüzyon metodu ile belirlenmiştir. Tüm izolatların penicillin G ve clindamycin’e karşı dirençli olduğu, diğer antibiyotiklere karşı ise değişen düzeylerde duyarlı olduğu tespit edilmiştir.

Keywords

Mavi yengeç, Vibrio parahaemolyticus, Antibiyotik dirençlilik, Halk sağlığı

Isolation, Identification and Determination of Some Antibiotics Resistance Profiles of Vibrio parahaemolyticus from Blue Crabs

Abstract

The purpose of this study is isolation, identification and determination of antibacterial resistance profiles of Vibrio parahaemolyticus from blue crabs obtained from Aydın province. Out of four isolates from sixty suspected isolates were identified as Vibrio parahaemolyticus by real-time PCR. The suspectibility level of these strains to antibiotics, such as Penicillin G, clindamycin, piperacilin, amoxicillin-clavulanic acid, ciprofloxacin and gentamicin was identified with disc diffusion method. It was determined all isolates were resistant to penicillin G and clindamycin and sensitive to other antibiotics at varying levels.

Keywords

Blue crabs, Vibrio parahaemolyticus, Antibiotic resistance, Public health

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