Mycobacterium avium subsp. paratuberculosis ile Enfekte Sığırlarda Bazı Akut Faz Protein ve Biyokimyasal Parametre Düzeylerinin Belirlenmesi

Year 2022, Volume: 3 Issue: 2, 47 - 51, 30.12.2022

Kadir Bozukluhan , Oğuz Merhan , Fatih Büyük , Enes Akyüz , Tahir Gezer , Hale Ergin Eğritağ , Gürbüz Gökçe

Abstract

Paratüberkülozis, kronik diyare ve granülomatöz enteritis ile karakterize evcil ve vahşi ruminantlarda görülen bulaşıcı bir hastalıktır. Çalışmanın amacı paratüberküloz ile enfekte sığırlarda bazı akut faz protein ve biyokimyasal parametre düzeylerinin belirlenmesidir. Çalışmada ELISA test sonuçlarına göre 15 paratüberküloz ile enfekte ve 15 antikor negatif sağlıklı sığır serumu kullanıldı. Serumlar haptoglobin, seruloplazmin, serum amiloid A (SAA), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), total protein, üre, kreatinin ve demir ölçümünde kullanıldı. Paratüberkülozisli sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında haptoglobin, SAA (P<0.001), üre (P<0.05), seruloplazmin, kreatinin düzeylerinin (P<0.01) ve bunun yanı sıra ALP ve GGT aktivitesinin (P<0.01) arttığı, albümin ve total protein düzeyinin (P<0.01) ise azaldığı belirlendi. Demir düzeyi kontrol grubuna göre azalmakla beraber istatistiksel olarak anlamsız tespit edildi. Sonuç olarak, elde edilen bulguların paratüberkülozisin teşhisine katkı sağlayacağı, haptoglobin ve SAA’nın hastalığın teşhisinde yardımcı parametre olarak kullanılabilecekleri ve bu konuda daha detaylı çalışmaların yapılması gerektiği düşünülmektedir.

Keywords

Sığır, Paratüberküloz, Akut faz protein, Biyokimyasal parametreler, Cattle, Paratuberculosis, Acute phase protein, Biochemical parameters

Determination of Some Acute Phase Protein and Biochemical Parameter Levels in Cattle Infected with Mycobacterium avium subsp. paratuberculosis

Abstract

Paratuberculosis is an infectious disease in domestic and wild ruminants characterized by chronic diarrhea and granulomatous enteritis. The aim of the study is to determine some acute phase protein and biochemical parameter levels in cattle infected with paratuberculosis. In the study, 15 paratuberculosis-infected and 15 antibody-negative healthy bovine serums were used according to ELISA test results. The sera were used for the measurement of haptoglobin, ceruloplasmin, serum amyloid A (SAA), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total protein, urea, creatinine and iron. When the cattle with paratuberculosis and the animals in the control group were compared, it was determined that haptoglobin, SAA (P<0.001), urea (P<0.05), ceruloplasmin, creatinine levels (P<0.01) as well as ALP and GGT activities (P<0.01) increased, while albumin and total protein levels (P<0.01) decreased. Although the iron level decreased compared to the control group, it was found to be statistically insignificant. As a result, it is thought that the obtained findings will contribute to the diagnosis of paratuberculosis, haptoglobin and SAA can be used as auxiliary parameters in the diagnosis of the disease, and more detailed studies should be done on this subject.

Keywords

Sığır, Paratüberküloz, Akut faz protein, Biyokimyasal parametreler, Cattle, Paratuberculosis, Acute phase protein, Biochemical parameters

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