Evaluation of Phytochemical Content and Pharmacological Effects of Laurel Essential Oil

Abstract

Laurus nobilis L., commonly known as laurel, is a species belonging to Lauraceae family and a characteristic plant of the Mediterranean climate. The plant is distributed in the coastal areas of our country, and the essential oil obtained from its leaves is used as a flavoring agent and spice in public. The studies conducted on the essential oil, which is rich in terpenic compounds, revealed various biological activities such as antimicrobial, antioxidant, insecticide, acaricidal, anticonvulsant, and cytotoxic activity. Approximately 322 components have been determined in the content of the essential oil and the major components were identified as 1,8-cineole, α-terpinyl acetate. In this review, it is aimed to present a scientific synthesis of the studies on the essential oil by emphasizing the traditional use, phytochemical content and biological activities of the essential oil obtained from the leaves of L. nobilis.

Keywords

• Biological Activity
• Laurel
• Laurus Nobilis
• Phytochemical Study
• Traditional Use

Defne Uçucu Yağının Fitokimyasal İçeriği ve Farmakolojik Etkilerinin Değerlendirilmesi

Abstract

Yaygın olarak defne adıyla bilinen Laurus nobilis L., Lauraceae familyasına ait bir tür olup, Akdeniz ikliminin karakteristik bir bitkisidir. Bitki, ülkemizde kıyı kesimlerinde yayılış göstermektedir ve yapraklarından elde edilen uçucu yağı, halk arasında gıdalarda aroma verici ve baharat olarak kullanılmaktadır. Yapılan çalışmalar sonucunda, terpenik bileşiklerce zengin uçucu yağının, antimikrobiyal, antioksidan, insektisit, akarisidal, antikonvülsan, sitotoksik vb. birçok biyolojik aktiviteye sahip olduğu gösterilmiştir. Uçucu yağın ana bileşiminde yaklaşık 322 bileşik tespit edilmiş olup, ana bileşenlerin 1,8-sineol, α-terpinil asetat olduğu belirlenmiştir. Bu derlemede, L. nobilis yapraklarından elde edilen uçucu yağın geleneksel kullanımı, fitokimyasal içeriği ve biyolojik aktiviteleri vurgulanarak, uçucu yağ hakkında yapılan çalışmaların bilimsel bir sentezinin sunulması amaçlanmaktadır.

Keywords

• Biyolojik Aktivite
• Fitokimyasal Çalışma
• Defne
• Geleneksel Kullanım
• Laurus Nobilis

References

1. Faydaoğlu, E., & Saip Sürücüoğlu, M. (2011). Geçmişten günümüze tıbbi ve aromatik bitkilerin kullanılması ve ekonomik önemi. Journal of Forestry Faculty, 11(1), 52–67.
2. Çırpan, M. (2017). Bursa–Kurşunlu yöresi Defne (Laurus nobilis L.) sahalarından farklı dönem ve yükseltilerde toplanan defne yapraklarının uçucu yağ verimi ve kimyasal bileşiminin incelenmesi (Yüksek Lisans Tezi). Bursa Teknik Üniversitesi, Fen Bilimleri Enstitüsü, Bursa.
3. Lubbe, A., & Verpoorte, R. (2011). Cultivation of medicinal and aromatic plants for specialty industrial materials. Industrial Crops and Products, 34(1), 785–801. https://doi.org/10.1016/J.INDCROP.2011.01.019
4. Gök, Ö., & Gıdık, B. (2019). Tıbbi ve aromatik bitkilerin kullanım alanları. Bayburt Üniversitesi Fen Bilimleri Dergisi, 2(1), 136–142.
5. Çelik, E., & Çelik, G. Y. (2007). Bitki uçucu yağlarının antimikrobiyal özellikleri. Orlab On-Line Mikrobiyoloji Dergisi, 05(2), 1–6.
6. Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils- A review. Food and Chemical Toxicology, 46(2), 446–475. https://doi.org/10.1016/J.FCT.2007.09.106
7. Pattabanoğlu, E. S. (2018). Laurus nobilis ve Cistus laurifolius’dan elde edilen uçucu yağların GC-MS analizi ve antimikrobiyal aktiviteleri (Yüksek Lisans Tezi). Kastamonu Üniversitesi Fen Bilimleri Enstitüsü, Kastamonu.
8. Yıldıztekin, M., Ulusoy, H., & Tuna, A. L. (2019). Türkiye’de tıbbi ve aromatik bitkilerin yetiştiriciliği ve sürdürülebilir gelişimi. In 4th International Symposium on Innovative Approaches in Engineering and Natural Sciences (pp. 481–484). Samsun: SETSCI. https://doi.org/10.36287/setsci.4.6.134

9. Yılmaz, A., & Çiftçi, V. (2021). Türkiye’de Defne (Laurus nobilis L.) bitkisinin durumu. European Journal of Science and Technology Special Issue, 22, 325–330. https://doi.org/10.31590/ejosat.856195
10. Karık, Ü., Çiçek, F., Oğur, E., Tutar, M., & Ayas, F. (2016). Türkiye’de yayılış gösteren Defne (Laurus nobilis L.) popülasyonlarının meyve özellikleri. Journal of AARI, 26(1), 1–16.
11. Conforti, F., Statti, G., Uzunov, D., & Menichini, F. (2006). Comparative chemical composition and antioxidant activities of wild and cultivated Laurus nobilis L. leaves and Foeniculum vulgare subsp. piperitum (Ucria) coutinho seeds. Biological and Pharmaceutical Bulletin, 29(10), 2056–2064. https://doi.org/10.1248/BPB.29.2056
12. Patrakar, R., Mansuriya, M., & Patil, P. (2012). Phytochemical and Pharmacological Review on Laurus Nobilis. International Journal of Pharmaceutical and Chemical Sciences, 1(2), 595–602.
13. Alejo-Armijo, A., Altarejos, J., & Salido, S. (2017). Phytochemicals and biological activities of Laurel Tree (Laurus nobilis). Natural Product Communications, 12(5), 743–757. https://doi.org/10.1177/1934578X1701200519
14. Mansour, O., Al-Abideen Douba, Z., & Ismail, G. (2018). Review study on the physiological properties and chemical composition of the Laurus nobilis. The Pharmaceutical and Chemical Journal, 5(1), 225–231.
15. Anzano, A., de Falco, B., Grauso, L., Motti, R., & Lanzotti, V. (2022). Laurel, Laurus nobilis L.: A review of its botany, traditional uses, phytochemistry and pharmacology. phytochemistry reviews. Springer Science and Business Media B.V. https://doi.org/10.1007/s11101-021-09791-z
16. Chahal, K. K., Kaur, M., Bhardwaj, U., Singla, N., & Kaur, A. (2017). A review on chemistry and biological activities of Laurus nobilis L. essential oil. Journal of Pharmacognosy and phytochemistry, 6(4), 1153–1161.
17. Mssillou, I., Agour, A., El Ghouizi, A., Hamamouch, N., Lyoussi, B., & Derwich, E. (2020). Chemical composition, antioxidant activity, and antifungal effects of essential oil from Laurus nobilis L. flowers growing in Morocco. Journal of Food Quality, 2020. https://doi.org/10.1155/2020/8819311
18. Özcan, A. (2020). Hatay halk kültüründe Defne Ağacı (Yüksek Lisans Tezi). Hatay Mustafa Kemal Üniversitesi Sosyal Bilimler Enstitüsü, Hatay.
19. Kaya, B., & Aladağ, C. (2009). Maki ve garig topluluklarının Türkiye’deki yayılış alanları ve ekolojik özelliklerinin incelenmesi. Selçuk Üniversitesi Sosyal Bilimleri Enstitüsü, 67–80.
20. Özer, S. (2021), Defne (Laurus nobilis L.) popülasyonuna ait tek bitkilerde bazı morfolojik, fizyolojik ve kalite özelliklerinin belirlenmesi (Yüksek Lisans Tezi). Ege Üniversitesi Fen Bilimleri Enstitüsü.
21. Davis PH. (1982). Flora of Turkey (Vol. 7). Edinburg: Edinburg Universty Pres.
22. Yilmaz, E. S., Timur, M., & Aslim, B. (2013). Antimicrobial, antioxidant activity of the essential oil of Bay Laurel from Hatay, Turkey. Journal of Essential Oil-Bearing Plants, 16(1), 108–116. https://doi.org/10.1080/0972060X.2013.764158
23. Moghtader, M., & Salari, H. (2012). Comparative survey on the essential oil composition from the leaves and flowers of Laurus nobilis L. from Kerman province. Journal of Ecology and the Natural Environment, 4(6), 150–153. https://doi.org/10.5897/JENE11.126
24. Kashkouli, S., Jamzad, M., & Nouri, A. (2018). Total phenolic and flavonoids contents, radical scavenging activity and green synthesis of silver nanoparticles by Laurus nobilis L. leaves aqueous extract. Journal of Medicinal Plants and By-products (Vol. 1).
25. Peixoto, L. R., Rosalen, P. L., Ferreira, G. L. S., Freires, I. A., de Carvalho, F. G., Castellano, L. R., & de Castro, R. D. (2017). Antifungal activity, mode of action and anti-biofilm effects of Laurus nobilis Linnaeus essential oil against Candida spp. Archives of oral biology, 73, 179–185. https://doi.org/10.1016/J.ARCHORALBIO.2016.10.013
26. Abdollahi Fard, M., & Shojaii, A. (2013). Efficacy of Iranian traditional medicine in the treatment of epilepsy. BioMed Research International, 2013, 1–8. https://doi.org/10.1155/2013/692751
27. Loizzo, M. R., Saab, A. M., Tundis, R., Statti, G. A., Menichini, F., Lampronti, I., & Doerr, H. W. (2008). Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species. Chemistry & Biodiversity, 5, 461–470. https://doi.org/10.1002/cbdv.200890045
28. Türk Farmakopesi 2017. (2018). Ankara: T.C. Sağlık Bakanlığı Yayınları, Türkiye İlaç ve Tıbbi Cihaz Kurumu.
29. Boza A., (2013) Karaburun Çeşme ve Dilek Yarımadası’nda Bulunan Doğal Defne (Laurus nobilis L.) Populasyonları Üzerinde Araştırmalar (Doktora tezi). Ege Üniversitesi Fen bilimleri Enstitüsü.
30. Yalçın, H., Akın, M., Şanda, M. A., & Çakır, A. (2007). Gas Chromatography/Mass Spectrometry Analysis of Laurus nobilis essential oil composition of Northern Cyprus. Journal of Medicinal Food, 10(4), 715–719. https://doi.org/10.1089/jmf.2007.404
31. El-Sawi, S. A., Elsayed, I. M., & Amal, M. A. (2009). In vitro cytotoxic, antioxidant and antimicrobial activities of essential oil of leaves of Laurus nobilis L. grown in Egypt and its Chemical Composition. Medicinal and aromatic plant science and biotechnology, 3(Special Issue 1), 16–23.
32. Ramos, C., Teixeira, B., Batista, I., Matos, O., Serrano, C., Neng, N. R., Marques, A. (2012). Antioxidant and antibacterial activity of essential oil and extracts of bay laurel Laurus nobilis Linnaeus (Lauraceae) from Portugal. Natural Product Research, 26(6), 518–529. https://doi.org/10.1080/14786419.2010.531478
33. Abu-Dahab, R., Kasabri, V., & Afifi, F. (2014). Evaluation of the volatile oil composition and antiproliferative activity of Laurus nobilis L. (Lauraceae) on breast cancer cell line models. Record of Natural Products, 8(2), 136–147.
34. Peris, I., & Blázquez, M. A. (2015). Comparative GC-MS analysis of bay leaf (Laurus nobilis L.) essential oils in commercial samples. International Journal of Food Properties, 18(4), 757–762. https://doi.org/10.1080/10942912.2014.906451
35. Pilar Santamarina, M., Roselló, J., Giménez, S., & Amparo Blázquez, M. (2016). Commercial Laurus nobilis L. and Syzygium aromaticum L. Merr. & Perry essential oils against post-harvest phytopathogenic fungi on rice. LWT, 65, 325–332. https://doi.org/10.1016/J.LWT.2015.08.040
36. Kıvrak, Ş., Göktürk, T., & Kıvrak, İ. (2017). Assessment of volatile oil composition, phenolics and antioxidant activity of bay (Laurus nobilis) leaf and usage in cosmetic Applications. International Journal of Secondary Metabolite, 4(2), 148–161. https://doi.org/10.21448/ijsm.323800
37. Dhifi, W., El Beyrouthy, M., Mnif, W., Pharm Sci, P. J., Bellili, S., Jazi, S., & Ben Nasr, S. (2018). Phytochemical composition and antioxidant activity of Tunisian Laurus nobilis. Article in Pakistan Journal of Pharmaceutical Sciences (Vol. 31).
38. Elkiran, O., Akbaba, E., & Bagci, E. (2018). Constituents of essential oils from leaves and seeds of Laurus nobilis L.: A chemotaxonomic approach. Bangladesh Journal of Botany, 47(4), 893–901. https://doi.org/10.3329/BJB.V47I4.47379
39. Ordoudi, S. A., Papapostolou, M., Kokkini, S., & Tsimidou, M. Z. (2020). Diagnostic Potential of FT-IR Fingerprinting in botanical origin evaluation of Laurus nobilis L. essential oil is supported by GC-FID-MS data. Molecules, 25(3). https://doi.org/10.3390/molecules25030583
40. Usmani, Q. I., Ahmad, A., & Jamaldeen, F. N. (2021). Laurus nobilis L., (Habb-ul-Ghar), a review on phytochemistry, pharmacology and ethnomedicinal uses. Journal of Drug Delivery and Therapeutics, 11(5), 136–144. https://doi.org/10.22270/jddt.v11i5.5021
41. Odeh, D., Oršolić, N., Berendika, M., Ðikić, D., Drozdek, S. D., Balbino, S., & Jurčević, I. L. (2022). Antioxidant and anti-atherogenic activities of essential oils from Myrtus communis L. and Laurus nobilis L. in rat. Nutrients, 14(7). https://doi.org/10.3390/nu14071465
42. Paparella, A., Nawade, B., Shaltiel-Harpaz, L., & Ibdah, M. (2022). A Review of the botany, volatile composition, biochemical and molecular aspects, and traditional uses of Laurus nobilis. Plants. https://doi.org/10.3390/plants11091209
43. Boulila, A., Hassen, I., Haouari, L., Mejri, F., Amor, I. ben, Casabianca, H., & Hosni, K. (2015). Enzyme-assisted extraction of bioactive compounds from bay leaves (Laurus nobilis L.). Industrial Crops and Products, 74, 485–493. https://doi.org/10.1016/j.indcrop.2015.05.050
44. Fidan, H., Stefanova, G., Kostova, I., Stankov, S., Damyanova, S., Stoyanova, A., & Zheljazkov, V. D. (2019). Chemical composition and antimicrobial activity of Laurus nobilis L. essential oils from Bulgaria. Molecules, 24(4). https://doi.org/10.3390/molecules24040804
45. Nabila, B., Piras, A., Fouzia, B., Falconieri, D., Kheira, G., Fedoul, F. F., & Majda, S. R. (2022). Chemical composition and antibacterial activity of the essential oil of Laurus nobilis leaves. Natural Product Research, 36(4), 989–993. https://doi.org/10.1080/14786419.2020.1839450
46. Siriken, B., Yavuz, C., & Guler, A. (2018). Antibacterial activity of Laurus nobilis: A review of literature. Medical Science and Discovery, 5(11), 374–379. https://doi.org/10.17546/msd.482929
47. Caputo, L., Nazzaro, F., Souza, L. F., Aliberti, L., de Martino, L., Fratianni, F., & De Feo, V. (2017). Laurus nobilis: Composition of essential oil and Its Biological Activities. Molecules, Vol. 22, Page 930, 22(6), 930. https://doi.org/10.3390/molecules22060930
48. Caredda, A., Marongiu, B., Porcedda, S., & Soro, C. (2002). Supercritical carbon dioxide extraction and characterization of Laurus nobilis essential oil. Journal of Agricultural and Food Chemistry, 50, 1492–1496. https://doi.org/10.1021/jf0108563
49. Da Silveira, S. M., Luciano, F. B., Fronza, N., Cunha, A., Scheuermann, G. N., & Vieira, C. R. W. (2014). Chemical composition and antibacterial activity of Laurus nobilis essential oil towards foodborne pathogens and its application in fresh Tuscan sausage stored at 7°C. LWT, 59(1), 86–93. https://doi.org/10.1016/J.LWT.2014.05.032
50. Verdian-rizi, M., & Hadjiakhoondi, A. (2008). Essential oil composition of Laurus nobilis L. of different growth stages growing in Iran. Zeitschrift fur Naturforschung - Section C, Journal of Biosciences, 63(11–12), 785–788. https://doi.org/10.1515/ZNC-2008-11-1201/machinereadablecitation/ris
51. Derwich, E., Benziane, Z., Boukir, A., Mohamed, S., & Abdellah, B. (2009). Chemical composition and antibacterial activity of leaves essential oil of Laurus nobilis from Morocco. Australian Journal of Basic and Applied Sciences, 3(4), 3818–3824.
52. Sangun, M., Aydın, E., Timur, M., Karadeniz, H., Çalışkan, M., & Özkan, A. (2007). Comparison of chemical composition of the essential oil of Laurus nobilis L. leaves and fruits from different regions of Hatay, Turkey. Journal of Environmental Biology, 28(4), 731–733.
53. Taban, A., Saharkhiz, M. J., & Niakousari, M. (2018). Sweet bay (Laurus nobilis L.) essential oil and its chemical composition, antioxidant activity and leaf micromorphology under different extraction methods. Sustainable Chemistry and Pharmacy, 9, 12–18. https://doi.org/10.1016/j.scp.2018.05.001
54. Řebíčková, K., Bajer, T., Šilha, D., Ventura, K., & Bajerová, P. (2020). Comparison of chemical composition and biological properties of essential oils obtained by hydrodistillation and steam distillation of Laurus nobilis L. Plant foods for human nutrition, 75(4), 495–504. https://doi.org/10.1007/S11130-020-00834-Y
55. Aurori, A. C., Bobiş, O., Dezmirean, D. S., Mărghitaş, L. A., & Erler, S. (2016). Bay laurel (Laurus nobilis) as potential antiviral treatment in naturally BQCV infected honeybees. Virus Research, 222, 29–33. https://doi.org/10.1016/j.vırusres.2016.05.024
56. Moghtader, M., & Farahmand, A. (2013). Evaluation of the antibacterial effects of essential oil from the leaves of Laurus nobilis L. in Kerman Province. Journal of Microbiology and Antimicrobials, 5(2), 13–17. https://doi.org/10.5897/JMA2012.0233
57. Ouibrahim, A., Tlili-Ait-Kaki, Y., Bennadja, S., Amrouni, S., Djahoudi, A. G., & Djebar, M. R. (2013). Evaluation of antibacterial activity of Laurus nobilis L., Rosmarinus officinalis L. and Ocimum basilicum L. from Northeast of Algeria. African Journal of. Microbiology Research , 7(42), 4968–4973. https://doi.org/10.5897/AJMR2012.2390
58. Abu-Zaid, A. A., Alopidi, M. A., & El-Sehrawy, M. H. (2013). In vitro antibacterial, anticancer and antioxidant properties of some oil plant extract. Journal of American Science, 9(11), 83–94.
59. Ivanović, J., Mišić, D., Ristić, M., Pešić, O., & Žižović, I. (2010). Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.)-chemical composition and antibacterial activity. The Journal of the Serbian Chemical Society, 75(3), 395–404. https://doi.org/10.2298/JSC090303003I
60. Nehir El, S., Karagozlu, N., Karakaya, S., & Sahın, S. (2014). Antioxidant and antimicrobial activities of essential oils extracted from Laurus nobilis L. leaves by using solvent-free microwave and hydrodistillation. Food and Nutrition Sciences, 5, 97–106. https://doi.org/10.4236/fns.2014.52013
61. Yerou Karima, O., & Touil, T. A. (2015). Physicochemical study and antibacterial activity of Laurus nobilis from Mascara (Algeria). Journal of Chemical and Pharmaceutical Research, 7(5), 1102–1106.
62. Ertürk, Ö., Aydın, G., & Çol Ayvaz, M. (2020). Laurus nobilis L., Silybum marianum L., Nigella sativa L. ve Prunus cerasus L.’den soğuk pres yöntemi ile izole edilen esansiyel yağ bileşenlerinin antimikrobial ve antioksidan aktiviteleri. Türk Tarım ve Doğa Bilimleri Dergisi, 487–499. https://doi.org/10.30910/turkjans.725987
63. Roselló, J., Sempere, F., Sanz-Berzosa, I., Chiralt, A., & Santamarina, M. P. (2015). Antifungal activity and potential use of essential oils against Fusarium culmorum and Fusarium verticillioides. Journal of Essential oil Plants, 18(2), 359–367. https://doi.org/10.1080/0972060X.2015.1010601
64. Li, Y.-X., Liu, M., Yang, K., Zheng, W., & Tian, J. (2022). Antimicrobial mechanisms of spice essential oils and application in food industry. Food Chemistry, 382, 1–14. https://doi.org/10.1016/j.foodchem.2022.132312
65. Guynot, M. E., Ramos, A. J., Setó, L., Purroy, P., Sanchis, V., & Marín, S. (2003). Antifungal activity of volatile compounds generated by essential oils against fungi commonly causing deterioration of bakery products. Journal of Applied Microbiology, 94(5), 893–899. https://doi.org/10.1046/j.1365-2672.2003.01927.x
66. Belasli, A., Ben Miri, Y., Aboudaou, M., Aït Ouahioune, L., Montañes, L., Ariño, A., & Djenane, D. (2020). Antifungal, antitoxigenic, and antioxidant activities of the essential oil from laurel (Laurus nobilis L.): Potential use as wheat preservative. Food Science and Nutrition, 8(9), 4717–4729. https://doi.org/10.1002/FSN3.1650
67. Reyes-Jurado, F., Franco-Vega, A., Ramírez-Corona, N., Palou, E., & López-Malo, A. (2015). Essential oils: antimicrobial activities, extraction methods, and their modeling. Food Engineering Reviews, 7, 275–297. https://doi.org/10.1007/s12393-014-9099-2
68. Singh A., Das S., Chaudhari A. K., Deepika, Soni M., Yadav A., Dwivedy A. K., Kishore Dubey N. (2023). Laurus nobilis essential oil nanoemulsion-infused chitosan: A safe and effective antifungal agent for masticatory preservation. Plant Nano Biology, 5, 1-11 https://doi.org/10.1016/j.plana.2023.100043.
69. Kurtfaki, M., & Yildirim-Yalcin, M. (2023). Characterization of Laurus nobilis L. leaf essential oil incorporated maize starch and rice protein films. Food Measure, 17, 4954–4962. https://doi.org/10.1007/s11694-023-02013-4
70. Kırışık M. (2022) Determination of fumigant toxicity of single, binary and tertiary mixtures of three essential oils against Frankliniella occidentalis Pergande (Thysanoptera: Thripidae). Research Square, 1-14.
71. Thoma, J. L., Cantrell, C. L., Tamang, P., & Zheljazkov, V. D. (2023). Determining the optimum mixture of three essential oils for potato sprout suppression at room temperature storage. Frontiers in Plant Science, 14, 1-12.
72. Nejad, Z. B., Nikrouz, L., & Abdollahi, A. (2023). Antibacterial Activity of Some Iranian Plant Essential Oils as Disinfectant Agents on Surfaces Contaminated with Staphylococcus Aureus and Pseudomonas Aeruginosa. Journal of Advanced Biomedical Sciences, 1-14.
73. Özcan, B., Esen, M., Kemal Sangun, M., Coleri, A., & Çalışkan, M. (2010). Effective antibacterial and antioxidant properties of methanolic extract of Laurus nobilis seed oil. Journal of Environmental Biology, 31(5), 637–641.
74. Başak Şahin, S., & Candan, F. (2013). Effect of Laurus nobilis L. essential oil and its main components on α-glucosidase and reactive oxygen species scavenging activity. Shaheed Beheshti University of Medical Sciences and Health Services. Iranian Journal of Pharmaceutical Research, 12(2), 367–379.
75. Kaur, M., Chahal, K., Kumar, A., Kaur, R., & Urvashi. (2018). Nematicidal activity of bay leaf (Laurus nobilis L.) essential oil and its components against Meloidogyne incognita. Journal of Entomology and Zoology Studies, 6(2), 1057–1064.
76. Macchioni, F., Perrucci, S., Cioni, P., Morelli, I., Castilho, P., & Cecchi, F. (2006). Composition and acaricidal activity of laurus novocanariensis and Laurus nobilis essential oils against Psoroptes cuniculi. Journal of Essential Oil Research, 18(1), 111–114. https://doi.org/10.1080/10412905.2006.9699403
77. Alimi, D., Hajri, A., Jallouli, S., & Sebai, H. (2021). In vitro acaricidal activity of essential oil and crude extracts of Laurus nobilis, (Lauraceae) grown in Tunisia, against arthropod ectoparasites of livestock and poultry: Hyalomma scupense and Dermanyssus gallinae. Veterinary Parasitology, 298. https://doi.org/10.1016/J.VETPAR.2021.109507
78. Perry, N., & Perry, E. (2006). Aromatherapy in the management of psychiatric disorders clinical and neuropharmacological perspectives. CNS Drugs, 20(4), 257–280.
79. Parichanon P., Ascrizzi R., Tani C., Sanmartin C., Taglieri I., Macaluso M., Flamini G.,Pieracci Y., Venturi F., & Conti B. (2023). The protective combined effect of chitosan and essential oil coatings on cheese and cured meat against the oviposition of Piophila casei, Food Bioscience, 56. https://doi.org/10.1016/j.fbio.2023.103132.
80. Gugliuzzo A., Francardi V., Simoni S., Roversi P. F., Ferrati M., Spinozzi E., Perinelli D. R., Bonacucina G., Maggi F., Tortorici S., Tropea Garzia G., Biondi A., & Rizzo R. (2023). Role of plant essential oil nanoemulsions on host colonization by the invasive ambrosia beetle Xylosandrus compactus, Industrial Crops and Products, 195, 1-14. https://doi.org/10.1016/j.indcrop.2023.116437.
81. Dos Santos A.R., Do Nascimento R.F.S.C., Da Cruz J.P., Da Silva Xavier A., De Souza Ramos A., Ferreira J.L.P., Maria A.C.B., De Almeida M.M.H., Da Silva M.A.M., Gomes Correa P., De Andrade Silva J.R., De Carvalho Queiroz M.M., Amaral A.C.F. (2023). Evaluation of essential oils and diluents against Chrysomya megacephala, an important mechanical vector, Journal of Natural Pesticide Research, 3,1-8 https://doi.org/10.1016/j.napere.2023.100024.
82. Hached W., Chamkhi A., Ncibi S. & Lebdi-Grissa K. (2023). Evaluation of Laurus nobilis essential oil repellent effect on the date moth Ectomyelois ceratoniae under controlled conditions. International Journal of Zoological and Entomological Letters. 3(1), 01-04.
83. Aıssaouı, L. Bouazız, A. Boudjelıda, & H. Nazlı, A. (2023) Phytochemıcal Screenıng And Bıologıcal Effects Of Laurus Nobılıs (Lauraceae) Essentıal Oıl Agaınst Mosquıto Larvae, Culex Pıpıens (Lınneaus, 1758) (Dıptera: Culıcıdae) Specıes. Applied Ecology And Environmental Research 21(1):287-300.
84. Jemaa, J. M.-B., Tersim, N., & Khouja, M. L. (2011). Composition and repellent efficacy of essential Oil from Laurus nobilis aganist adults of the cigarette beetle Lasioderma serricorne (Coleoptera: Anobiidae). Tunisian Journal of Plant Protection, 6(1), 29–42.
85. Mediouni Ben Jemâa, J., Tersim, N., Toudert, K. T., & Khouja, M. L. (2012). Insecticidal activities of essential oils from leaves of Laurus nobilis L. from Tunisia, Algeria and Morocco, and comparative chemical composition. Journal of Stored Products Research, 48, 97–104. https://doi.org/10.1016/J.JSPR.2011.10.003
86. Andronikashvili, M., & Reichmuth, C. (2003). Repellency and toxicity of essential oils from Ocimum gratissimum (Lamiaceae) and Laurus nobilis (Lauraceae) from Georgia against the rust-red flour beetle (Tribolium castaneum Herbst) (Coleoptera: Tenebrionidae). In Advances in stored product protection. Proceedings of the 8th International Working Conference on Stored Product Protection, York, UK, 22-26 July 2002 (pp. 749–762). CABI. https://doi.org/10.1079/9780851996912.0749
87. Chahal, K. K., Singh, D. K., Panchbhaiya, A., Singh, N., Kaur, M., Bhardwaj, U., & Kaur, A. (2017). A review on chemistry and biological activities of Laurus nobilis L. essential oil. Journal of Pharmacognosy and Phytochemistry, 6(4).
88. El Faqer O, Rais S., Elkoraichi I., El Amrani A., Dakir M., Zaid Y., & Mtairag E. M., (2023). Phytochemical characterization and immunomodulatory effects of aqueous and ethanolic extracts and essential oil of Moroccan Laurus nobilis L. (Lauraceae) on human neutrophils. Journal of Herbmed Pharmacology. 12(1): 92-99.
89. Sayyah, M., Saroukhani, G., Peirovi, A., & Kamalinejad, M. (2003). Analgesic and anti-inflammatory activity of the leaf essential oil of Laurus nobilis Linn. Phytotherapy Research, 17, 733–736. https://doi.org/10.1002/ptr.1197
90. Ferreira, A., Proença, C., Serralheiro, M. L. M., & Araújo, M. E. M. (2006). The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. Journal of Ethnopharmacology, 108(1), 31–37. https://doi.org/10.1016/J.JEP.2006.04.010
91. Smach M.A., Hafsa J., Abdallah J.B., Charfeddine B., Limem K. (2024). Neuroprotective and anti-amnesic effects of Laurus Nobilis essential oil against scopolamine-induced memory deficits in mice brain, Journal of Ethnopharmacology, 319. https://doi.org/10.1016/j.jep.2023.117151.
92. Yazici, M., Mazlum, Y., Naz, M., Urku, C., Turkmen, M., & Akayli, T. (2022). Effects of adding laurel (Laurus nobilis) essential oil to the diet of tilapia fish on growth and intestinal histology. Aquatic Sciences and Engineering, 37(4), 195-204. DOI: https://doi.org/10.26650/ASE20221101489
93. Sebai, E., Abidi, A., Benyedem, H., Dhibi, M., Hammemi, I., & Akkari, H. (2022). Phytochemical profile and anthelmintic effects of Laurus nobilis essential oil against the ovine nematode Haemonchus contortus and the murine helminth model Heligmosomoides polygyrus. Veterinary Parasitology, 312.
94. Fornari, T., Vicente, G., Vázquez, E., García-Risco, M. R., & Reglero, G. (2012). Isolation of essential oil from different plants and herbs by supercritical fluid extraction. Journal of Chromatography A, 1250, 34–48. https://doi.org/10.1016/j.chroma.2012.04.051
95. Legault, J., Dahl, W., Debiton, E., Pichette, A., & Madelmont, J. C. (2003). Antitumor activity of balsam fir oil: Production of reactive oxygen species induced by alpha-humulene as possible mechanism of action. Planta medica, 69(5), 402–407. https://doi.org/10.1055/S-2003-39695
96. Sylvestre, M., Pichette, A., Longtin, A., Nagau, F., & Legault, J. (2006). Essential oil analysis and anticancer activity of leaf essential oil of Croton flavens L. from Guadeloupe. Journal of Ethnopharmacology, 103(1), 99–102. https://doi.org/10.1016/J.JEP.2005.07.011