Solanum lycopersicum Yaprak Ekstraktının Farklı İnsan Hücre Hatlarındaki Sitotoksik Potansiyeli

Abstract

Bitkiler geleneksel olarak çeşitli hastalıklara karşı çare olarak kullanılmaktadır. Bu çalışmada Solanum lycopersicum yapraklarının etanol ekstraktının potansiyel sitotoksik etkileri A549, HeLa, PC-3, MCF-7 ve HEK293 hücre hatlarında araştırılmıştır. Hücre canlılığının belirlenmesi için MTT deneyi kullanılmıştır. Ekstarakt ile muamele edilen A549 ve HeLa hücrelerinde apoptoz ve kaspaz-3 aktivitesi araştırılmıştır. 31.25 µg mL-1 konsantrasyonda, bu ekstrakt, 72 saatte test edilen kanser hücrelerinde % 50'den fazla sitotoksisiteye neden olmuştur. S. lycopersicum ekstraktının A549 ve HeLa hücrelerinde apoptozu indüklediği belirlenmiştir. Ayrıca, S. lycopersicum yapraklarının ekstresi aynı hücrelerde önemli kaspaz-3 aktivitesine neden olmuştur. Bu bulgular temel olarak antikanser ajanların gelişimi için S. lycopersicum yaprak ekstraktının kullanımı ile ilgili araştırmalara katkıda bulunabilir.

Keywords

• sitotoksisite
• domates
• insan kanseri hücre hatları
• Apoptoz

Cytotoxic Potential of Solanum lycopersicum Leaves Extract on Different Human Cell Lines

Abstract

Plants have been traditionally used as a remedy against various diseases. In this study, the potential cytotoxic effects of the ethanol extract of leaves of Solanum lycopersicum were investigated on A549, HeLa, PC-3, MCF-7 and HEK293 cell lines. MTT assay was used for determination of cell viability. Apoptosis and activity of caspase-3 in A549 and HeLa cells treated with the extract were also investigated. At a concentration of 31.25 µg mL-1, this extract caused more than 50% cell death in the cancer cells that were tested for 72 h. It was determined that the extract of S. lycopersicum induced apoptosis in A549 and HeLa cells. Moreover, the extract of S. lycopersicum leaves caused significant caspase-3 activity in same cells. These findings may basically contribute to research related with the use of the extract of leaves of S. lycopersicum for development of anticancer agents.

Keywords

• cytotoxicity
• tomato
• human cancer cell lines
• Apoptosis

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