Solanum lycopersicum Yaprak Ekstraktının Farklı İnsan Hücre Hatlarındaki Sitotoksik Potansiyeli

Öz

Bitkiler geleneksel olarak çeşitli hastalıklara karşı çare olarak kullanılmaktadır. Bu çalışmada Solanum lycopersicum yapraklarının etanol ekstraktının potansiyel sitotoksik etkileri A549, HeLa, PC-3, MCF-7 ve HEK293 hücre hatlarında araştırılmıştır. Hücre canlılığının belirlenmesi için MTT deneyi kullanılmıştır. Ekstarakt ile muamele edilen A549 ve HeLa hücrelerinde apoptoz ve kaspaz-3 aktivitesi araştırılmıştır. 31.25 µg mL-1 konsantrasyonda, bu ekstrakt, 72 saatte test edilen kanser hücrelerinde % 50'den fazla sitotoksisiteye neden olmuştur. S. lycopersicum ekstraktının A549 ve HeLa hücrelerinde apoptozu indüklediği belirlenmiştir. Ayrıca, S. lycopersicum yapraklarının ekstresi aynı hücrelerde önemli kaspaz-3 aktivitesine neden olmuştur. Bu bulgular temel olarak antikanser ajanların gelişimi için S. lycopersicum yaprak ekstraktının kullanımı ile ilgili araştırmalara katkıda bulunabilir.

Anahtar Kelimeler

• sitotoksisite
• domates
• insan kanseri hücre hatları
• Apoptoz

Cytotoxic Potential of Solanum lycopersicum Leaves Extract on Different Human Cell Lines

Öz

Plants have been traditionally used as a remedy against various diseases. In this study, the potential cytotoxic effects of the ethanol extract of leaves of Solanum lycopersicum were investigated on A549, HeLa, PC-3, MCF-7 and HEK293 cell lines. MTT assay was used for determination of cell viability. Apoptosis and activity of caspase-3 in A549 and HeLa cells treated with the extract were also investigated. At a concentration of 31.25 µg mL-1, this extract caused more than 50% cell death in the cancer cells that were tested for 72 h. It was determined that the extract of S. lycopersicum induced apoptosis in A549 and HeLa cells. Moreover, the extract of S. lycopersicum leaves caused significant caspase-3 activity in same cells. These findings may basically contribute to research related with the use of the extract of leaves of S. lycopersicum for development of anticancer agents.

Anahtar Kelimeler

• cytotoxicity
• tomato
• human cancer cell lines
• Apoptosis

Kaynakça

1. Kooti, W., Servatyari, K., Behzadifar, M., Asadi-Samani, M., Sadeghi, F., Nouri, B., & Zare Marzouni, H. (2017). Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study. Journal of evidence-based complementary & alternative medicine, 22(4), 982-995
2. Kinghorn, D. (2000). Plant secondary metabolites as potential anticancer agents and cancer chemopreventives. Molecules, 5, 285-288.
3. Yang, L., Wen, K. S., Ruan, X., Zhao, Y. X., Wei, F., & Wang, Q. (2018). Response of Plant Secondary Metabolites to Environmental Factors. Molecules (Basel, Switzerland), 23(4), 762.
4. Martí, R., Roselló, S., & Cebolla-Cornejo, J. (2016). Tomato as a source of carotenoids and polyphenols targeted to cancer prevention. Cancers (Basel), 8(6), 58.
5. Gerszberg, A., Hnatuszko-Konka, K., Kowalczyk, T., & Kononowicz, A. K. (2015). Tomato (Solanum lycopersicum L.) in the service of biotechnology. Plant Cell, Tissue Organ Culture, 120(3), 881-902.
6. Navarro-González, I., García-Alonso, J., & Periago, M. J. (2018). Bioactive compounds of tomato: Cancer chemopreventive effects and influence on the transcriptome in hepatocytes. Journal of Functional Foods, 42, 271-280.
7. Saturnino, C., Spagnuolo, A., Palladino, C., Popolo, A., Tommonaro, G., De Prisco, R., & Pinto, A. (2013). Antiproliferative activity of “Lycopersicon esculentum” leaves (var. Paul Robenson): Preliminary study. Food and Nutrition Sciences, 4, 632-635.
8. Chik, W.D.W., Amid, A., & Jamal, P. (2010). Purification and cytotoxicity assay of tomato (Lycopersicon esculen tum) leaves methanol extract as potential anticancer agent. Journal of Applied Sciences, 10(24), 3283-3288.

9. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of lmmunological Methods, 65, 55-63.
10. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytıcal Biochemistry, 72, 248-254
11. Greenwell, M., & Rahman, P.K.S.M. (2015). Medicinal Plants: Their use in anticancer treatment. International Journal of Pharmaceutical Sciences and Research, 6(10), 4103-4112.
12. Mbaveng, A. T., Manekeng, H. T., Nguenang, G. S., Dzotam, J. K., Kuete, V., & Efferth, T. (2018). Cytotoxicity of 18 Cameroonian medicinal plants against drug sensitive and multi-factorial drug resistant cancer cells. Journal of Ethnopharmacology, 222, 21-33.
13. Nguenang, G. S., Ntyam, A., & Kuete, V. (2020). Acute and Subacute Toxicity Profiles of the Methanol Extract of Lycopersicon esculentum L. Leaves (Tomato), a Botanical with Promising In Vitro Anticancer Potential. Evidence-Based Complementary and Alternative Medicine : eCAM, 2020, 8935897.
14. Pfeffer, C. M., & Singh, A. T. K. (2018). Apoptosis: A target for anticancer therapy. International Journal of Molecular Sciences, 19(2), 448.
15. Hwang, E.-S., & Bowen, P. E. (2005). Effects of tomato paste extracts on cell proliferation, cell-cycle arrest and apoptosisin LNCaP human prostate cancer cells. BioFactors, 23, 75-84.
16. Holzapfel, N. P., Holzapfel, B. M., Champ, S., Feldthusen, J., Clements, J., & Hutmacher, D. W. (2013). The potential role of lycopene for the prevention and therapy of prostate cancer: From molecular mechanisms to clinical evidence. International Journal of Molecular Sciences, 14(7), 14620-14646.
17. Soares, N.C.P., Machado, C.L., Trindade, B.B., Lima, I.C.C., Gimba, E.R.P., Teodoro, A.J., Takiya, C., & Borojevic, R. (2017). Lycopene extracts from different tomato-based food products induce apoptosis in cultured human primary prostate cancer cells and regulate TP53, Bax and Bcl-2 transcript expression. Asian Pacific Journal of Cancer Prevention, 18(2), 339-345.
18. Barone, D., Cito, L., Tommonaro, G., Abate, A.A., Penon, D., De Prisco, R., Penon, A., Forte, I.M., Benedetti, E., Cimini, A., Indovina, P., Nicolaus, B., Pentimalli, F., & Giordano, A. 2018. Antitumoral potential, antioxidant activity and carotenoid content of two Southern Italy tomato cultivars extracts: San Marzano and Corbarino. Journal of Cellular Physiology, 233, 1266-1277.
19. Porter, A.G., & Jänicke, R.U. (1999). Emerging roles of caspase-3 in apoptosis. Cell Death & Differentiation, 6, 99-104.
20. Sundquist T., Moravec, R. , Niles, A., O'Brien, M., Riss T., Promega Corporation (2006). Timing your apoptosis assays. Cell Notes 16, 18-21.