Effect of Electrochemically Reduced Graphene Oxide, Polythionine and Gold Nanoparticles on Electrooxidation of Dopamine, NADH and Ascorbic Acid

Abstract

In this study, screen-printed carbon electrodes (SPCE) were modified with electrochemically reduced graphene oxide (ERGO) (SPCE/ERGO), then polythionine (PTH) film was formed on SPCE/ERGO by electropolymerization technique (SPCE/ERGO/PTH). The optimum number of cycles has been determined for the electropolymerization of PTH on SPCE/ERGO. Gold nanoparticles (AuNP) were formed on SPCE/ERGO/PTH electrodes by electrodeposition method and electrochemical characterizations of SPCE/ERGO/PTH/AuNP electrodes were made by cyclic voltammetry (CV). The electrocatalytic effects of ERGO, PTH and AuNP on the electrooxidation of dopamine (DA), nicotinamide adenine dinucleotide (NADH) and ascorbic acid (AA) were investigated, and PTH was observed to play a role as a mediator for three analytes. The anodic peak potentials of DA, NADH and AA shifted in the negative direction, and anodic peak currents increased due to the synergistic effect of ERGO, PTH and AuNP together. The diffusion coefficients (D) of DA, NADH and AA were calculated as 3.37×10−8, 7.79×10−9 and 1.51×10−8, respectively, and also the electron transfer coefficients (α) were calculated as 0.327, 0.701 and 0.373, respectively. The effect of pH on the electrooxidation of DA, NADH and AA was also examined, and it was observed that the anodic peak potentials for all three analytes decreased with increasing the pH.

Keywords

• Dopamine
• NADH
• Ascorbic Acid
• Electrooxidation

Dopamin, NADH ve Askorbik Asitin Elektroyükseltgenmesine Elektrokimyasal Olarak İndirgenmiş Grafen Oksit, Politiyonin ve Altın Nanopartiküllerin Etkisi

Abstract

Bu çalışmada, yüzey baskılı karbon elektrotlar (SPCE) elektrokimyasal olarak indirgenmiş grafen oksit (ERGO) ile modifiye edilmiş (SPCE/ERGO), ardından elektropolimerizasyon tekniği ile politiyonin (PTH) filmi SPCE/ERGO’lar üzerinde oluşturulmuştur (SPCE/ERGO/PTH). PTH’nin SPCE/ERGO’lar üzerindeki elektropolimerizasyonu için optimum döngü sayısı belirlenmiştir. SPCE/ERGO/PTH elektrotları üzerinde elektrodepozisyon metodu ile altın nanopartiküller (AuNP) oluşturulmuş ve elde edilen SPCE/ERGO/PTH/AuNP elektrotlarının elektrokimyasal karakterizasyonları dönüşümlü voltametri (CV) ile yapılmıştır. Dopamin (DA), nikotinamid adenin dinükleotitin indirgenmiş formu (NADH) ve askorbik asitin (AA) elektroyükseltgenmesine ERGO, PTH ve AuNP’nin elektrokatalitik etkileri incelenmiş ve PTH’nin üç analit için de medyatör olarak rol oynadığı gözlenmiştir. ERGO, PTH ve AuNP’nin birlikte gösterdikleri sinerjik etki ile DA, NADH ve AA’nın anodik pik potansiyelleri negatif yöne kaymış ve anodik pik akımları artmıştır. DA, NADH ve AA’nın difüzyon katsayıları (D) sırasıyla 3.37×10−8, 7.79×10−9 ve 1.51×10−8 olarak, elektron transfer katsayıları (α) ise sırasıyla 0.327, 0.701 ve 0.373 olarak hesaplanmıştır. pH’nın DA, NADH ve AA’nın elektroyükseltgenmesine etkisi de incelenmiş ve pH arttıkça her üç analit için de anodik pik potansiyellerinin azaldığı gözlenmiştir.

Keywords

• Dopamin
• NADH
• Askorbik asit
• Elektroyükseltgenme

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