2-(karbazol-3’-il)-5-formilpiridin: Floresans Biyotiyol Sensör Özelliğinin İncelenmesi

Year 2021, Volume: 8 Issue: 2, 901 - 912, 31.12.2021

Ahmet Battal , Nuray Altınölçek

https://doi.org/10.35193/bseufbd.976953

Abstract

Bu çalışmada 2-(karbazol-3’-il)-5-formilpiridin molekülü floresans prob olarak biyotiyol (sistein, homosistein ve glutatyon) moleküllerinin tayin ve tespitinde kullanıldı. Probun bu biyotiyollere karşı duyarlılık ve seçiciliği UV-vis ve PL spektrometreleri ile incelendi. Prob, 1:9 DMSO:HEPES Tampon çözeltisi (0.1 M, pH:7.4) içerisinde biyotiyoller arasından sisteine karşı 60 eşdeğerde emisyon artışı ile seçicilik gösterdi. Ayrıca prob ile biyotiyoller arasında oluşan yeni yapı 1H NMR ve MS spektrometreleri ile de takip edilerek teyit edildi.

Keywords

Aldehit Grup, Biyotiyol, Fiziksel Özellikler, Karbazol, Sensör

Supporting Institution

TÜBİTAK

Project Number

1059B191800354

Thanks

A.B. ve N.A. değerli katkı, teşvik ve yardımlarından dolayı Uludağ Üniversitesi Fen-Edebiyat Fakültesi Kimya Bölümü’nden Prof. Dr. Mustafa Tavaslı’ya çok teşekkür eder. A.B., 2219 kodlu Yurtdışı Doktora Sonrası Araştırma Burs (Burs Numarası:1059B191800354) kapsamında destek veren TÜBİTAK’a teşekkür eder. Ayrıca A.B. laboratuvar imkanlarını sonuna kadar kullanıma açıp yardımcı olan Glasgow Üniversitesi’nden Prof. Dr. Peter Skabara’ya ve Duetta Spektrometresiyle ölçüm imkânı tanıyan Dr. William Peveler’e minnettardır. Yine A.B., tüm değerli fikir ve yardımları için Glasgow Üniversitesi’nden Dr. Oleksandr’a, Dr. Holly A. Yu’a ve Hao Yang’a teşekkür eder.

2-(carbazole-3'-yl)-5-formylpyridine: Investigation of the Fluorescence Biothiol Sensor Property

Abstract

In this study, 2-(carbazole-3'-yl)-5-formylpyridine molecule was used as a fluorescence probe for the determination and detection of biothiol (cysteine, homocysteine and glutathione) molecules. The sensitivity and selectivity of the probe to these biothiols were examined with UV-vis and PL spectrometers. The probe showed selectivity among biothiols in 1:9 DMSO:HEPES solution (0.1 M, pH:7.4) with an emission increase of 60 equivalents against cysteine. In addition, the new structure formed between the probe and the biothiols was confirmed by monitoring with 1H NMR and MS spectrometers.

Keywords

Aldehyde Group, Biothiol, Physical Properties, Carbazole, Sensor

Project Number

1059B191800354

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