Apoptotic And Antiangiogenic Effects of Noscapine In Human Lung Cancer (A549) Cells

Öz

Objective: Lung cancer, one of the most important health problems today, is the most common type of cancer that causes death in both women and men. Although A549 lung cells originating from human alveolar carcinoma cells match the type II alveolar cell phenotype, it also has many characteristics that human primary alveolar epithelial cells have. Noscapine consists of the components in the poppy somniferum (opium). It was first isolated from Papaver somniferum (opium) in 1817. It consists of the most abundant opioids found in the opium plant (up to 10% of the total composition) after morphine. It is also known as Narcotine, Nectodon, Nospen, Anarcotine and (archaic) Opiane, and S is an (-) isomer with R stereochemistry (S stereochemistry in phthalid-carbon and R in isoquinoline-carbon). Noscapine is structurally and chemically different from other opium alkaloids such as morphine, codeine, thebaine, papaverine and narcein.The aim of the study the ınvestıgatıon of the apoptotıc and antıangıogenıc effects of noscapine ın human lung cancer (A549) cells. Materials and Methods: In the study, concentrations of 10 ppm, 15 ppm, 20 ppm, 25 ppm, 40 ppm, 50 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 90 ppm and 100 ppm were used in the proliferation experiment proportion of vitality values. It was observed that it decreased and the LD50 value was determined as 40 ppm. Statistical analysis used: Statistical analysis of the analyzes was performed using SPSS Statistics 20,0 program. Results: VEGF values decreased at 20 ppm, 40 ppm and 80 ppm noscapine concentrations compared to the control group. There was a significant decrease in PARP values at 80 ppm. Conclusions: As a result of the findings; It is thought that concentrations of noscapine 10 ppm, 20 ppm and 40 ppm can reduce angiogenesis and prevent metastasis by lowering VEGF levels. PARP levels decreased at all noscapine concentrations but the most significant difference was seen at 80 ppm noscapine concentration.

Anahtar Kelimeler

• A549
• Noscapine
• PARP
• Papaver Somniferum
• VEGF

insan akciğer hücre hatlarında noskapinin antianjiogenik ve apopitotik etkileri

Öz

Giriş ve Amaç: Günümüzün en önemli sağlık sorunlarından biri olan akciğer kanseri hem kadınlarda hem de erkeklerde en sık ölüme neden olan kanser türüdür. İnsan alveolar karsinom hücrelerinden kaynaklanan A549 akciğer hücreleri, tip II alveolar hücre fenotipiyle eşleşmesine rağmen, insan primer alveolar epitel hücrelerinin sahip olduğu birçok özelliğe de sahiptir. Noskapin, haşhaş somniferum (afyon) içindeki bileşenlerden oluşur. İlk olarak 1817'de Papaver somniferum'dan (afyon) izole edilmiştir. Morfinden sonra afyon bitkisinde (toplam bileşimin %10'una kadar) bulunan en bol opioidlerden oluşur. Narcotine, Nectodon, Nospen, Anarcotine ve (arkaik) Opiane olarak da bilinir ve S, R stereokimyasına sahip bir (-) izomerdir (fitalid-karbonda S stereokimyası ve izokinolin-karbonda R). Noskapin yapısal ve kimyasal olarak morfin, kodein, tebain, papaverin ve narsein gibi diğer afyon alkaloidlerinden farklıdır. Bu çalışmanın amacı, insan akciğer kanseri (A549) hücrelerinde noskapin'in apoptotik ve antianjiogenik etkilerinin araştırılmasıdır. Gereç ve Yöntemler: Çalışmada 10 ppm, 15 ppm, 20 ppm, 25 ppm, 40 ppm, 50 ppm, 60 ppm, 65 ppm, 70 ppm, 75 ppm, 80 ppm, 90 ppm ve 100 ppm konsantrasyonları kullanılmıştır. Bulgular: Canlılık değerlerinin çoğalma deneyi oranı. Düştüğü gözlendi ve LD50 değeri 40 ppm olarak belirlendi. Kullanılan istatistiksel analiz: Analizlerin istatistiksel analizi SPSS Statistics 20,0 programı kullanılarak yapıldı. Bulgular: VEGF değerleri 20 ppm, 40 ppm ve 80 ppm noskapin konsantrasyonlarında kontrol grubuna göre azaldı. 80 ppm'de PARP değerlerinde önemli bir düşüş oldu. Sonuç: Bulgular sonucunda; Noskapin konsantrasyonlarının 10 ppm, 20 ppm olduğu düşünülmektedir. 40 ppm, VEGF seviyelerini düşürerek anjiyogenezi azaltabilir ve metastazı önleyebilir. PARP seviyeleri tüm noskapin konsantrasyonlarında azaldı, ancak en önemli fark 80 ppm'de görüldü.

Anahtar Kelimeler

• A549
• Noscapine
• PARP
• Papaver Somniferum
• VEGF

Kaynakça

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