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FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ

Year 2020, Volume: 7 Issue: 3, 257 - 265, 30.09.2020
https://doi.org/10.34087/cbusbed.672920

Abstract

Kadın infertilitesinin sebeplerinden biri olan prematür over yetmezliğinin (POY) bilinen ve bilinmeyen birçok sebebi vardır. Kemoterapötiklerin ve bazı kimyasal ajanların özellikle gonadotoksik etkileri ovaryumları etkileyerek ovaryumda fonksiyon kaybına ve sonucunda POY’u tetikleyerek infertiliteye sebep olmaktadır. POY’un deneysel çalışmalarında hastalığın klinikteki semptomlarına en uygun deneysel modeli oluşturmak en etkili tedavilerin geliştirilebilmesi çok önemlidir. Bu çalışmanın amacı POY indükleyen iki önemli ajan 4-vinilsiklohekzen diepoksid ve siklofosfamid’in POY oluşturma mekanizmalarını histopatolojik olarak değerlendirmektir. Bu amaçla 12 haftalık erişkin Wistar albino cinsi dişi sıçanlar 4-vinilsiklohekzen diepoksid ve siklofosfamide maruz bırakıldı. Deney sonucu elde edilen ovaryum dokularında hematoksilen eozin, masson trikrom boyaması yapıldı ve ovaryum dokusu morfolojik olarak incelendi. Ovaryumda ki histopatolojik parametreler skorlandı ve kontrol gruplarıyla kıyaslandı. 4-vinilsiklohekzen diepoksid grubu ovaryum dokusunda kontrol ve siklofosfamid grubuna göre artmış vasküler konjesyon, nötrofil infiltrasyonu ve vakualizasyon gözlendi. Aynı zamanda atretik folikül sayısı kontrol grubuna kıyasla artmıştı. Siklofosfamid grubunda 4- vinilsiklohekzen diepoksid grubuna göre çok az konjesyon gözlendi, ancak kontrol grubuna kıyasla gelişmekte olan foliküllerde atrezi, foliküller içerisinde artmış vakuolizasyon görüldü. Sonuç olarak her iki ajanın da ovaryumda POY’u tetiklediği ancak birbirinden farklı bazı histopatolojik durumlar oluşturduğu gözlendi.

Supporting Institution

Manisa Celal Bayar Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2019-021

References

  • 1. Mascarenhas, M. N., Flaxman, S. R., Boerma, T., Vanderpoel, S., & Stevens, G. A. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS medicine, (2012), 9(12), e1001356.
  • 2. WHO Scientific Group on Recent Advances in Medically Assisted Conception, & World Health Organization. Recent advances in medically assisted conception: report of a WHO scientific group (No. 820). World Health Organization, (1992).
  • 3. Nelson, L. M. Primary ovarian insufficiency. New England Journal of Medicine, (2009), 360(6), 606-614.
  • 4. Jankowska, K. Premature ovarian failure. Przeglad menopauzalny= Menopause review, (2017), 16(2), 51.
  • 5. Hoyer, P.B., Sipes, I.G.. Development of an animal model for ovotoxicity using 4-vinylcyclohexene: a case study. Birth Defects Res. B. Dev. Reprod. Toxicol. (2007), 80, 113e125
  • 6. Zhang, Ting, et al. "The comparison of animal models for premature ovarian failure established by several different source of inducers." Regulatory Toxicology and Pharmacology 81 (2016): 223-232.
  • 7. Çelik, M, Sıçanlarda 4-vınylcyclohexene dıepoxıde (vcd) ile olusturulan deneysel prematür over yetmezliği’ne silymarin’in etkisi, Osmangazi Üniversitesi, Eskişehir, 2009, p20-24.
  • 8. Kappeler, Connie J., and Patricia B. Hoyer. "4-vinylcyclohexene diepoxide: a model chemical for ovotoxicity." Systems biology in reproductive medicine 58.1 (2012): 57-62.
  • 9. Liu, Wei, et al. "Conditions and Possible Mechanisms of VCD-induced Ovarian Failure." Alternatives to Laboratory Animals 43.6 (2015): 385-392.
  • 10. Kao, S.W., Sipes, I.G. & Hoyer, P.B. (1999). Early effects of ovotoxicity induced by 4-vinylcyclohexene diepoxide in rats and mice. Reproductive Toxicology 13, 67–75.
  • 11. Jeelani, R., Khan, S. N., Shaeib, F., Kohan-Ghadr, H. R., Aldhaheri, S. R., Najafi, T., ... & Abu-Soud, H. M. (2017). Cyclophosphamide and acrolein induced oxidative stress leading to deterioration of metaphase II mouse oocyte quality. Free Radical Biology and Medicine, 110, 11-18.
  • 12. Emadi, A., Jones, R. J., & Brodsky, R. A. (2009). Cyclophosphamide and cancer: golden anniversary. Nature reviews Clinical oncology, 6(11), 638.
  • 13. Sahambi, Sukhdeep K., et al. "Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice." Reproductive Toxicology 26.2 (2008): 116-122.
  • 14. Ling, L., Feng, X., Wei, T., Wang, Y., Wang, Y., Zhang, W., ... & Xiong, Z. (2017). Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats. Stem cell research & therapy, 8(1), 283.
  • 15. IHCWORLD Life Science Products & Services. IHC WORLD, LLC. https://www.ihcworld.com/_protocols/special_stains/h&e_ellis.htm (accessed 15.10.2019)
  • 16. IHCWORLD Life Science Products & Services. IHC WORLD, LLC. http://www.ihcworld.com/_protocols/special_stains/masson_trichrome.htm (accessed 15.10.2019)
  • 17. Springer LN, McAsey ME, Flaws JA, Tilly JL, Sipes IG, Hoyer PB. Involvement of apoptosis in 4-vinylcyclohexene diepoxide-induced ovotoxicity in rats. Toxicol Appl Pharmacol 1996;139:394–401.
  • 18. Devine, Patrick J., I. Glenn Sipes, and Patricia B. Hoyer. "Initiation of delayed ovotoxicity by in vitro and in vivo exposures of rat ovaries to 4-vinylcyclohexene diepoxide." Reproductive Toxicology 19.1 (2004): 71-77.
  • 19. Zhou, Linyan, et al. "Rapamycin prevents cyclophosphamide-induced over-activation of primordial follicle pool through PI3K/Akt/mTOR signaling pathway in vivo." Journal of ovarian research 10.1 (2017): 56.
  • 20. Mayer LP, Pearsall NA, Christian PJ, Devine PJ, Payne CM, McCuskey MK, et al. Long-term effects of ovarian follicular depletion in rats by 4-vinylcyclohexene diepoxide. Reprod Toxicol 2002;16:775–81
  • 21. Mark-Kappeler, C.J., Sen, N., Keating, A.F., Sipes, I.G., Hoyer, P.B., 2010. Distribution and responsiveness of rat anti-Müllerian hormone during ovarian development and VCD-induced ovotoxicity. Toxicol. Appl. Pharmacol. 249, 1e7.
  • 22. Hu XM, Christian PJ, Thompson KE, Sipes IG, Hoyer PB. Apoptosis induced in rats by 4-vinylcyclohexene diepoxide is associated with activation of the caspase cascades. Biol Reprod 2001;65:87–93.
  • 23. Devine PJ, Sipes IG, Hoyer PB. Effect of 4-vinylcyclohexene diepoxide dosing in rats on GSH levels in liver and ovaries. Toxicol Sci 2001;62:315–20.
Year 2020, Volume: 7 Issue: 3, 257 - 265, 30.09.2020
https://doi.org/10.34087/cbusbed.672920

Abstract

Project Number

2019-021

References

  • 1. Mascarenhas, M. N., Flaxman, S. R., Boerma, T., Vanderpoel, S., & Stevens, G. A. National, regional, and global trends in infertility prevalence since 1990: a systematic analysis of 277 health surveys. PLoS medicine, (2012), 9(12), e1001356.
  • 2. WHO Scientific Group on Recent Advances in Medically Assisted Conception, & World Health Organization. Recent advances in medically assisted conception: report of a WHO scientific group (No. 820). World Health Organization, (1992).
  • 3. Nelson, L. M. Primary ovarian insufficiency. New England Journal of Medicine, (2009), 360(6), 606-614.
  • 4. Jankowska, K. Premature ovarian failure. Przeglad menopauzalny= Menopause review, (2017), 16(2), 51.
  • 5. Hoyer, P.B., Sipes, I.G.. Development of an animal model for ovotoxicity using 4-vinylcyclohexene: a case study. Birth Defects Res. B. Dev. Reprod. Toxicol. (2007), 80, 113e125
  • 6. Zhang, Ting, et al. "The comparison of animal models for premature ovarian failure established by several different source of inducers." Regulatory Toxicology and Pharmacology 81 (2016): 223-232.
  • 7. Çelik, M, Sıçanlarda 4-vınylcyclohexene dıepoxıde (vcd) ile olusturulan deneysel prematür over yetmezliği’ne silymarin’in etkisi, Osmangazi Üniversitesi, Eskişehir, 2009, p20-24.
  • 8. Kappeler, Connie J., and Patricia B. Hoyer. "4-vinylcyclohexene diepoxide: a model chemical for ovotoxicity." Systems biology in reproductive medicine 58.1 (2012): 57-62.
  • 9. Liu, Wei, et al. "Conditions and Possible Mechanisms of VCD-induced Ovarian Failure." Alternatives to Laboratory Animals 43.6 (2015): 385-392.
  • 10. Kao, S.W., Sipes, I.G. & Hoyer, P.B. (1999). Early effects of ovotoxicity induced by 4-vinylcyclohexene diepoxide in rats and mice. Reproductive Toxicology 13, 67–75.
  • 11. Jeelani, R., Khan, S. N., Shaeib, F., Kohan-Ghadr, H. R., Aldhaheri, S. R., Najafi, T., ... & Abu-Soud, H. M. (2017). Cyclophosphamide and acrolein induced oxidative stress leading to deterioration of metaphase II mouse oocyte quality. Free Radical Biology and Medicine, 110, 11-18.
  • 12. Emadi, A., Jones, R. J., & Brodsky, R. A. (2009). Cyclophosphamide and cancer: golden anniversary. Nature reviews Clinical oncology, 6(11), 638.
  • 13. Sahambi, Sukhdeep K., et al. "Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice." Reproductive Toxicology 26.2 (2008): 116-122.
  • 14. Ling, L., Feng, X., Wei, T., Wang, Y., Wang, Y., Zhang, W., ... & Xiong, Z. (2017). Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats. Stem cell research & therapy, 8(1), 283.
  • 15. IHCWORLD Life Science Products & Services. IHC WORLD, LLC. https://www.ihcworld.com/_protocols/special_stains/h&e_ellis.htm (accessed 15.10.2019)
  • 16. IHCWORLD Life Science Products & Services. IHC WORLD, LLC. http://www.ihcworld.com/_protocols/special_stains/masson_trichrome.htm (accessed 15.10.2019)
  • 17. Springer LN, McAsey ME, Flaws JA, Tilly JL, Sipes IG, Hoyer PB. Involvement of apoptosis in 4-vinylcyclohexene diepoxide-induced ovotoxicity in rats. Toxicol Appl Pharmacol 1996;139:394–401.
  • 18. Devine, Patrick J., I. Glenn Sipes, and Patricia B. Hoyer. "Initiation of delayed ovotoxicity by in vitro and in vivo exposures of rat ovaries to 4-vinylcyclohexene diepoxide." Reproductive Toxicology 19.1 (2004): 71-77.
  • 19. Zhou, Linyan, et al. "Rapamycin prevents cyclophosphamide-induced over-activation of primordial follicle pool through PI3K/Akt/mTOR signaling pathway in vivo." Journal of ovarian research 10.1 (2017): 56.
  • 20. Mayer LP, Pearsall NA, Christian PJ, Devine PJ, Payne CM, McCuskey MK, et al. Long-term effects of ovarian follicular depletion in rats by 4-vinylcyclohexene diepoxide. Reprod Toxicol 2002;16:775–81
  • 21. Mark-Kappeler, C.J., Sen, N., Keating, A.F., Sipes, I.G., Hoyer, P.B., 2010. Distribution and responsiveness of rat anti-Müllerian hormone during ovarian development and VCD-induced ovotoxicity. Toxicol. Appl. Pharmacol. 249, 1e7.
  • 22. Hu XM, Christian PJ, Thompson KE, Sipes IG, Hoyer PB. Apoptosis induced in rats by 4-vinylcyclohexene diepoxide is associated with activation of the caspase cascades. Biol Reprod 2001;65:87–93.
  • 23. Devine PJ, Sipes IG, Hoyer PB. Effect of 4-vinylcyclohexene diepoxide dosing in rats on GSH levels in liver and ovaries. Toxicol Sci 2001;62:315–20.
There are 23 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Araştırma Makalesi
Authors

Busra Sen 0000-0003-4089-5243

Mehmet İbrahim Tuğlu 0000-0002-0569-8415

Project Number 2019-021
Publication Date September 30, 2020
Published in Issue Year 2020 Volume: 7 Issue: 3

Cite

APA Sen, B., & Tuğlu, M. İ. (2020). FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 7(3), 257-265. https://doi.org/10.34087/cbusbed.672920
AMA Sen B, Tuğlu Mİ. FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. September 2020;7(3):257-265. doi:10.34087/cbusbed.672920
Chicago Sen, Busra, and Mehmet İbrahim Tuğlu. “FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7, no. 3 (September 2020): 257-65. https://doi.org/10.34087/cbusbed.672920.
EndNote Sen B, Tuğlu Mİ (September 1, 2020) FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7 3 257–265.
IEEE B. Sen and M. İ. Tuğlu, “FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ”, CBU-SBED: Celal Bayar University-Health Sciences Institute Journal, vol. 7, no. 3, pp. 257–265, 2020, doi: 10.34087/cbusbed.672920.
ISNAD Sen, Busra - Tuğlu, Mehmet İbrahim. “FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 7/3 (September 2020), 257-265. https://doi.org/10.34087/cbusbed.672920.
JAMA Sen B, Tuğlu Mİ. FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2020;7:257–265.
MLA Sen, Busra and Mehmet İbrahim Tuğlu. “FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ”. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol. 7, no. 3, 2020, pp. 257-65, doi:10.34087/cbusbed.672920.
Vancouver Sen B, Tuğlu Mİ. FARKLI İKİ YÖNTEMLE OLUŞTURULAN DENEYSEL PREMATÜR OVER YETMEZLİK MODELİNDE OVERLERİN HİSTOPATOLOJİK DEĞERLENDİRİLMESİ. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2020;7(3):257-65.

Cited By

Diyabetik Sıçan Deri Yara İyileşmesinde Probiyotik Etkisi
Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi
https://doi.org/10.34087/cbusbed.1078611