Jasminum officinale Linn.'nin (Oleaceae) Bazı Biyoaktiviteleri ve Koku Bileşenlerinin Araştırılması: Kozmetik Ürün Tasarımı için Değerli bir Araç

Abstract

Bu çalışmada Muğla ve çevresinde doğal olarak yetişen Jasminum officinale Linn. çiçeğinin proteaz enzim aktivitesi üzerine araştırmalar yapılmıştır. Ayrıca yasemin çiçeğinin içeriğindeki koku bileşenleri tespit edilmiştir. Yasemin çiçeğinin diğer çiçeklerle olan uyumu ve nota kavramı esas alınarak parfüm yapımında kullanılması amaçlanmıştır. Proteaz enzimi, J. officinale çiçeğinden ÜFA (Üç fazlı sistem) yöntemi kullanılarak saflaştırıldı. Enzim için optimum pH ve optimum sıcaklık, kazein, azokazaein, jelatin, hemoglobin, azoalbümin substratları için Km ve Vmax değerleri belirlendi. J. officinale çiçeğinden saflaştırılan proteaz enziminin saflığını kontrol etmek için SDS-PAGE kullanıldı. Enzimin moleküler ağırlığı jel filtrasyon kromatografisi kullanılarak 21.386 kDa olarak hesaplandı. Fenolik içeriği belirlendi. Kozmetik sektörünün en prestijli ürünü olan parfüm tasarımında yasemin çiçeğinin içeriğinin kullanılabileceği belirlendi.

Keywords

• Kozmetik
• Yasemin çiçeği
• proteaz enzimi
• üç fazlı sistem

Investigation of Some Bioactivities and Odor Components of Jasminum officinale Linn. (Oleaceae): A Valuable Tool for Cosmetic Product Design

Abstract

In this study, researches were carried out on the protease enzyme activity of Jasminum officinale Linn. flower which grows naturally in Muğla and its surroundings. In addition, fragrance components in the content of jasmine flower were determined. It was aimed to be used in perfume making based on the harmony of white jasmine flower with other flowers and the concept of note. Protease enzyme was purified from J. officinale flower using TPP (Three Phase Partitioning) method. Optimal pH and optimal temperature for the enzyme, Km and Vmax values for casein, azokazaein, gelatin, hemoglobin, and azoalbumin substrates were determined. SDS-PAGE was used to check the purity of the protease enzyme purified from the J. officinale. The molecular weight of the enzyme was calculated as 21.386 kDa using gel filtration chromatography. The phenolic content was also determined. It has been determined that the content of jasmine flower can be used in perfume design which is the most prestigious product of the cosmetic industry.

Keywords

• Cosmetics
• Jasmine flower
• protease enzyme
• three phase system

References

1. Alrashdi, A.S., Salama, S.M., Alkiyumi, S.S., Abdulla, M.A., Hadi, A.H., Abdelwahab, S.I., Taha, M.M., Hussiani, J., & Asykin, N. (2012). Mechanisms of Gastroprotective Effects of Ethanolic Leaf Extract of Jasminum sambac against HCl/Ethanol-Induced Gastric Mucosal Injury in Rats. Evidence-Based Complementary and Alternative Medicine, 2012, 786426, 1-15. https://doi.org/10.1155/2012/786426
2. Angelescu, E., Pavel, O.D., Bırjega, R., Zavoianu, R., Costentin, G., & Che, M. (2006). Solid base catalysts obtained from hydrotalcite precursors, for Knoevenagel synthesis of cinamic acid and coumarin derivatives. Applied Catalysis A: General, 308, 13-18. https://doi.org/10.1016/j.apcata.2006.04.011
3. Arctander, S. (1994). Perfume and Flavor Chemicals (Aroma Chemicals). Allured Publishing Corporation; Carol Stream, Illinois.
4. Asif-Ullah, M., Kim, K.S., & Yu, Y.G. (2006). Purification and characterization of a serine protease from Cucumis trigonus Roxburghi. Phytochemistry, 67(9), 870-875.
5. Aşık, S. (2017). Beyaz Yasemin Çiçeğinin (Jasminum officinale) Bazı Biyoaktiviteleri ile Koku Bileşenlerinin Araştırılması ve Parfüm Tasarımında Kullanılması. Retrieved from: https://tez.yok.gov.tr/UlusalTezMerkezi/tezDetay.jsp?id=YMYx1FtU5Irj-u1RU7QajQ&no=sHf1HfMghp84EAgyB0b6HQ
6. Atrooz, O.M., & Alomari, F.N. (2020). Determination of the activity and kinetics parameters of proteases in the crude plant extracts of Mentha piperita L. and Thymus capitatus L. Journal of Applied Biology & Biotechnology, 8(6), 33-37. https://doi.org/10.7324/JABB.2020.80606
7. Bai, N., He, K., Ibarra, A., Bily, A., Roller, M., Chen, X., & Rühl, R. (2010). Iridoids from Fraxinus excelsior with adipocyte differentiation-inhibitory and PPARα activation activity. Journal of Natural Products, 73, 2-6. https://doi.org/10.1021/np9003118
8. Balkrishna, A., Sharma, G., Sharma, N., Rawat, N., Kumar, A., & Arya, V. (2022). Transition of Indian Agriculture from Glorious Past to Challenging Future: A Serious Concern. Indian Journal of Ecology, 49(3), 977-986. https://doi.org/10.55362/IJE/2022/3625.

9. Banik, S., Biswas, S., & Karmakar, S. (2018). Extraction, purification, and activity of protease from the leaves of Moringa oleifera [version 1; referees: 2 approved, 1 approved with reservations]. F1000Research, 7, 1151. https://doi.org/10.12688/f1000research.15642.1
10. Baytop, T. (1999). Türkiye’de Bitkiler İle Tedavi, Baskı II, Nobel Tıp Kitabevleri LTD., İstanbul, 234 ss.
11. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254. https://doi.org/10.1006/abio.1976.9999
12. Can, Z., Yildiz, O., Sahin, H., Turumtay, E.A., Silici, S., & Kolayli, S. (2015). An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry,180, 133-141. https://doi.org/10.1016/j.foodchem.2015.02.024
13. Chaiwut, P., Pintathong, P., & Rawdkuen, S. (2010). Extraction and three-phase partitioning behavior of proteases from papaya peels. Process Biochemistry, 45, 1172-1175. https://doi.org/10.1016/j.procbio.2010.03.019
14. Chaturverdi, A.P, Kumar, M. & Tripathi, Y.B. (2013). Efficacy of Jasminum grandiflorum L. Leaf extract od dermal wound healing in rats. International Wound Journal, 10(6), 675-82. https://doi.org/10.1111/j.1742-481X.2012.01043.x
15. Cheng, Z., Li, J.F., Niu, Y., Zhang, X.C., Woody, O.Z., Xiong, Y., Djonović, S., Millet, Y., Bush, J. & McConkey, B.J. (2015). Pathogen-secreted proteases activate a novel plant immune pathway. Nature, 521(7551), 213-216.
16. Chinese Pharmacopoeia Commission. (2015). Pharmacopoeia of the People's Republic of China, vol. 1, China Medical Science Publisher, Beijing, 45-46 (&271).
17. Daşdemir, S.N. (2017). Müge Çiçeğinin (Convallaria majalis) Bazı Biyoaktivitelerinin Belirlenmesi Ve Diğer Bazı Çiçeklerle Birlikte Parfüm Tasarımında Kullanılabilirliğinin Araştırılması. Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezDetay.jsp?id=4bBx1zmuywZwb8LkKyUN-w&no=0EfeptaMkhl8uE62XxAb4A
18. De Feo, V., Aquino, R., Menghini, A., Ramundo, E. & Senatore, F. (1992). Traditional Phytotherapy in the peninsula Sorrentina, Campania, Southern Italy. Journal of Ethnopharmacology, 36, 113-125. https://doi.org/10.1016/0378-8741(92)90010-O
19. Dennison, C., & Lovrein, R. (1997). Three phase partitioning: concentration and purification of proteins. Protein Expression and Purification, 11, 149-161. https://doi.org/10.1006/prep.1997.0779
20. Domsalla, A., & Melzig, M.F. (2008). Occurrence and properties of proteases in plant latices. Planta Medica, 74(07), 699-711. https://doi.org/10.1055/s-2008-1074530
21. Dossou-Yovo, H.O., Kindomihou, V., Vodouhè Fifanou, G., & Sinsin, B. (2021). Assessment of the diversity of medico-magic knowledge on four herbaceous species in Benin. The Scientific World Journal, 6650704, 1- 11. https://doi.org/10.1155/2021/6650704
22. Dossou-Yovo, H.O., Kindomihou, V., Vodouhè Fifanou, G., & Sinsin, B. (2022a). Investigating the use profile of Kigelia africana (Lam.) Benth. through market in Benin. Conservation, 2(2), 275-285. https://doi.org/10.3390/conservation2020019
23. Dossou-Yovo, H.O., Vodouhè Fifanou, G., Kaplan, A., & Sinsin, B., (2022b). Application of ethnobotanical indices in the utilization of five medicinal herbaceous plant species in Benin, West Africa. Diversity, 14, 612. https://doi.org/10.3390/d14080612
24. Elhawary, S., EL-Hefnawy, H., & Mokhtar, F.A. (2020). Green synthesis of silver nanoparticles using extract of Jasminum officinale L. leaves and evaluation of cytotoxic activity towards bladder (5637) and breast cancer (MCF-7) cell lines. International Journal of Nanomedicine, 15, 9771-9781. https://doi.org/10.2147/IJN.S269880
25. Fadıloğlu, S. (2001). Immobilization and characterization of ficin. Nahrung/Food, 45 (2), 143-146. https://doi.org/10.1002/1521-3803(20010401)45:2<143::AID-FOOD143>3.0.CO;2-8
26. Fares, R., Bazzi, S., Baydoun, S.E. & Abdel-Massih, R.M. (2011). The antioxidant and antiproliferative activity of the Lebanese Olea europaea extract. Plant Foods for Human Nutrition, 66, 58-63. https://doi.org/10.1007/s11130-011-0213-9
27. González, R.N., Badillo, J.A., Aranda, A.J.S., & Carmen O.S.M. (2011). Production of plant proteases in vivo and in vitro-a review. Biotechnology Advances, 29(6), 983-996. https://doi.org/10.1016/j.biotechadv.2011.08.017
28. Green, P.S. (2003). Synopsis of the Oleaceae from the Indian sub-continent. Kew Bulletin, 58(2), 257-295. https://doi.org/10.2307/4120616
29. Huang, Yan-Li., Oppong, M.B., Guo, Y., Wang, Li-Zhi., Fang, Shi-Ming., Deng, Yan-Ru., & Gao, Xiu-Mei. (2019). The Oleaceae family: A source of secoiridoids with multiple biological activities. Fitoterapia, 136,104155. https://doi.org/10.1016/j.fitote.2019.04.010
30. Jinka, R., Ramakrishna, V., Rao, S.K., & Rao, R.P. (2009). Purification and characterization of cysteine protease from germinating cotyledons of horse gram. BMC Biochemistry, 10(1), 1-11. https://doi.org/10.1186/1471-2091-10-28
31. Joshi, M.C., Raju, A., Arulanandham, A., & Saraswathy, G.R. (2008). Hepatoprotcetive activity of Jasminum angustifolium Linn against CCl4 induced hepatic injury in rat. Pharmacologyonline, 3, 197-205.
32. Kamatou, G.P.P., & Viljoen A.M. (2008). Linalool - A Review of a Biologically Active Compound of Commercial Importance. Natural Product Communications, 3(7), 1183-1192. https://doi.org/10.1177/1934578X0800300727
33. Karkaş, T. (2009). İnvertaz Enziminin Ekstraksiyonu ve Saflaştırılması için Sulu İkili-Faz Afinite Sistemlerinin Geliştirilmesi. Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/tezDetay.jsp?id=H8tgyFc-BcxsxgrIA0Gz2g&no=7EkHfFxCHwM0PT6-p1vR-Q
34. Kat, B., Yılmazer Keskin S. (2013). Üçlü faz sistemi ile saflaştırılan invertaz enziminin termal kararlılığının incelenmesi. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 17 (2), 291-294.
35. Kunhachan, P., Banchonglikitkul, C., Kajsongkram, T., Khayungarnnawee A., & Leelamanit, W. (2012). Chemical Composition, Toxicity and Vasodilatation Effect of the Flowers Extract of Jasminum sambac (L.) Ait. Evidence-Based Complementary and Alternative Medicine, 2012, 471312, 1-7. https://doi.org/10.1155/2012/471312
36. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680-85.
37. Mandujano Gonzalez, V., Téllez, J.A., Anducho, R.M.A., Arana, C.A., & Mercado, F.Y. (2016). Purification and characterization of the extracellular aspartyl protease APSm1 from the phytopathogen fungus Stenocarpella maydis. Protein Expression and Purification, 117,1-5. https://doi.org/10.1016/j.pep.2015.09.017
38. Niemer, M., Mehofer, U., Verdianz, M., Porodko, A., Schähs, P., Kracher, D., Lenarcic, B., Novinec, M., & Mach, L. (2016). Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease. Biochimie, 12(2), 119-125. https://doi.org/10.1016/j.biochi.2015.06.017
39. Parlak, B., Güngör, A. A., Demir, Y., & Demir, N. (2008). Anadolu Orkidesi (Orchis anatolica) Çiçeklerinden Proteaz Enziminin Saflaştırılması ve Peynir Üretimindeki Kullanılabilirliğinin Araştırılması. Türkiye 10. Gıda Kongresi, Mayıs 21-23, Erzurum, Türkiye.
40. Pauling, L. (1973). Die Natur der chemischen Bindung, Chemie-Varlag, Weinheim, 523 pp.
41. Peana, T.A., & Moretti, M.D.L. (2008). Linalool in Essential Plant Oils: Pharmacological Effects. Botanical medicine in clinical practice, 716-724, CAB International, Wallingford, UK. https://doi.org/10.1079/9781845934132.0716
42. Peana, A.T., D’Aquila, P.S., Panin, F., Serra, G., Pippia, P., & Moretti, M.D.L. (2002). Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils. Phytomedicine, 9, 721-726. https://doi.org/10.1078/094471102321621322
43. Peana, A.T., Marzocco, S., Popolo, A., & Pinto, A. (2006a). (−)-Linalool inhibits in vitro NO formation: Probable involvement in the antinociceptive activity of this monoterpene compound. Life Sciences, 78, 719-723. https://doi.org/10.1016/j.lfs.2005.05.065
44. Peana, A.T., Rubattu, P., Piga, G.G., Fumagalli, S., Boatto, G., Pippia, P., & De Montis, M.G. (2006b). Involvement of adenosine A1 and A2A receptors in (−)-linalool-induced antinociception. Life Sciences, 78, 2471-2474. https://doi.org/10.1016/j.lfs.2005.10.025
45. Prabhu, S., D., Apoorva, S., Nandita, J., Palani, C., & Devi Rajeswari, V. (2018). Purification of protease enzyme from the leaf, seed and pod samples of Vicia faba L. International Food Research Journal, 25(5), 1904-1911.
46. Prachee, D., Ayushi, T., Praveen, T., Shikha, A., A.K, R., & Watal, G. (2019). Phytochemical and Phytoelemental Profile of J. officinale. International Journal of Pharmacognosy and Phytochemical Research, 11(1), 5-9.
47. Rawdkuen, S., Chawut, P., Pntathong, P., & Benjakul, S. (2010). Three-Phase Partitioning of Protease from Calotropis procera latex. Biochemical Engineering Journal, 50, 145-149.
48. Rawlings, N.D., Barrett, A.J., & Bateman, A. (2010). MEROPS: The peptidase database. Nucleic Acids Research, 38(1), 227-233. https://doi.org/10.1093/nar/gkp971
49. Reshma, D., Anitha, C.T., & Tharakan, S.T. (2021). Phytochemical and Pharmacological Properties of Five Different Species of Jasminum. Plant Archives, 21(2), 126-136. https://doi.org/10.51470/PLANTARCHIVES.2021.v21.no2.022
50. Sabharwal, S., Aggarwal, S., Vats, M., & Sardana, S. (2012). Preliminary phytochemical investigation and wound healing activity of Jasminum sambac (linn) ait. (Oleaceae) Leaves. International Journal of Pharmacognosy and Phytochemical Research, 4(3), 146-150.
51. Sahu, R., Arya, V., Joshi, R., Kaushik, P., & Chauhan, M. (2022). Pharmacological and Therapeutic Properties of Jasminum officinale. L: A Review. Indian Journal of Ecology, 49(3) (SI), 1122-1128. https://doi.org/10.55362/IJE/2022/3640
52. Sequeiros, C., Torres, M.J., Nievas, M.L., Caffini, N.O., Natalucci, C.L., López, L.M., & Trejo, S.A. (2016). The Proteolytic Activity of Philibertia gilliesii Latex. Purification of Philibertain g II. Applied Biochemistry and Biotechnology, 179(2), 322-346. https://doi.org/10.1007/s12010-016-1997-8
53. Sõukand, R., Hrynevich, Y., Vasilyeva, I., Prakofjewa, J., Vnukovich, Y., Paciupa, J., Hlushko, A., Knureva, Y., Litvinava, Y., Vyskvarka, S., Silivonchyk, H., Paulava, A., Kõiva, M., & Kalle, R. (2017). Multi-functionality of the few: current and past uses of wild plants for food and healing in Liubań region, Belarus. Journal of Ethnobiology and Ethnomedicine, 13(10), https://doi.org/10.1186/s13002-017-0139-x
54. Van Zyl, R.L., Seatlholo, S.T., van Vuuren, S.F., &Viljoen, A.M. (2006). The biological activities of 20 nature identical essential oil. Journal of Essential Oil Research, 18, 129-133. https://doi.org/10.1080/10412905.2006.12067134
55. Wallander, E., & Albert, V.A. (2000). Phylogeny and Classification of Oleaceae Based on rps 16 and trnL-F Sequence Data, American Journal of Botany, 87(12),1827-1841. https://doi.org/10.2307/2656836
56. Yaltırık, F. (1978). Olea L., In: Flora of Turkey and East Aegean Islands (Ed: P.H. Davis), Vol 6, Edinburgh, 155-156.