Evaluation of the Effect of Thymol on the Cytotoxicity of Cetuximab in Lung Cancer Cells

Abstract

The treatment of lung cancer continues to be a significant challenge for many oncologists and their patients. Treatment using epidermal growth factor receptor inhibitors is connected to a positive outcome. Cetuximab, a chimeric monoclonal antibody targeting the epidermal growth factor receptor (EGFR), in conjunction with monoterpene phenol thymol, is recommended for the treatment of lung cancer. While a mild acne-like skin rash is quite frequent in patients using cetuximab, a severe rash is rare. The goal of the current study was to assess whether thymol could enhance the anticancer effectiveness of cetuximab in A-549, non-small cell lung cancer (NSCLC) cell line. We found that the combination of cetuximab and thymol synergistically suppressed cell proliferation by inducing membrane damaging, oxidative stress, and apoptosis in A-549 cells. Taken together, our results indicate that the combination of thymol and cetuximab could improve anticancer responses and may notably enhance treatment outcomes in NSCLC.

Keywords

• Cetuximab
• carvacrol
• lung cancer
• synergistic anticancer effect
• apoptosis

Supporting Institution

Alanya Alaaddin Keykubat University Scientific Research Projects Unit (2023-02-07-LAP01)

Project Number

2023-02-07-LAP01

Ethical Statement

Ethics committee approval is not required for this study.

Thanks

The authors wish to thanks to Alanya Alaaddin Keykubat University for providing the necessary facilities to conduct this study. The author would like to thank Alanya Alaaddin Keykubat University Scientific Research Projects Unit (2023-02-07-LAP01) for financial support of this work.

Timol’ün Akciğer Kanseri Hücrelerinde Cetuximab'ın Sitotoksisitesi Üzerindeki Etkisinin Değerlendirilmesi

Abstract

Akciğer kanserinin tedavisi, birçok onkolog ve hasta için önemli bir zorluk olmaya devam etmektedir. Epidermal büyüme faktörü reseptörü inhibitörleri kullanarak yapılan tedaviden olumlu bir sonuçlar alınmaktadır. Epidermal büyüme faktörü reseptörünü (EGFR) hedefleyen bir kimerik monoklonal antikor olan cetuximab, monoterpen fenol timol ile birlikte, akciğer kanserinin tedavisi için önerilmektedir. Cetuximab kullanan hastalarda hafif akne benzeri cilt döküntüsü oldukça sık görülürken, şiddetli döküntü nadir görülmektedir. Mevcut çalışmanın amacı, timolün cetuximabın A-549, küçük hücreli olmayan akciğer kanseri (NSCLC) hücre dizisindeki antikanser etkinliğini artırıp artırmayacağını değerlendirmektir. Cetuximab ve timol kombinasyonunun A-549 hücrelerinde zar hasarını, oksidatif stresi ve apoptozu uyararak hücre proliferasyonunu sinerjik bir şekilde baskıladığını bulduk. Bir arada değerlendirildiğinde, bulgularımız timol ve cetuximab kombinasyonunun antikanser yanıtların iyileştirilebileceğini ve NSCLC'deki tedavi sonuçlarının önemli ölçüde arttırılabileceğini göstermektedir.

Keywords

• Cetuximab
• karvakrol
• akciğer kanseri
• sinerjik antikancer etki
• apoptoz

Supporting Institution

Alanya Alaaddin Keykubat Üniversitesi Bilimsel Araştırma Projeleri Birimi (2023-02-07-LAP01)

Project Number

2023-02-07-LAP01

Ethical Statement

Bu çalışma için etik kurul onayı gerekmemektedir.

Thanks

Bu çalışmayı yürütmek için gerekli olanakları sağladığı için Alanya Alaaddin Keykubat Üniversitesi'ne ve bu çalışmanın finansal desteği için Alanya Alaaddin Keykubat Üniversitesi Bilimsel Araştırma Projeleri Birimi'ne (2023-02-07-LAP01) teşekkür ederiz

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