Cezayir Zizyphus lotus Özütlerinin LC–MS/MS Profillemesi ve Bunların in vitro Antioksidan Aktivitesi: Karşılaştırmalı Bir Çalışma

Abstract

Bu çalışmada, Cezayir'de halk tıbbında geleneksel olarak kullanılan Zizyphus lotus'un yaprak ve meyve ekstraktlarının antioksidan aktivitelerinin ve kimyasal bileşiminin belirlenmesi amaçlanmıştır. Toplam fenolik içeriği (TPC) ve toplam flavonoid içeriği (TFC) sırasıyla kolorimetrik test ile belirlenmiştir. Ekstraktlar LC-MS/MS ile analiz edilmiştir. Ayrıca, çeşitli ekstraktların antioksidan potansiyeli, 2,2-difenil-1-pikrilhidrazil (DPPH) deneyi, ferrik indirgeyici antioksidan güç (FRAP) deneyi ve 2,2-azinobis-etilbenzotiyozolin-6-sülfonik asit (ABTS) ve Fenantrolin aktivitesi gibi farklı antioksidan deneyler kullanılarak UV spektrofotometresi kullanılarak değerlendirilmiştir. Polifenol ve flavonoid içeriğinin kantitatif analizi yaprakların en yüksek miktarda olduğunu göstermiştir (sırasıyla 279.07±18.84 mg GAE/g E ve 132.74±27.74 mg ER/g E). En yüksek antioksidan aktivite 24.10±3.49 µg/mL, 9.365 ± 0.532 µg/mL, 0.19±0.093, 0.636±0.033 mg AAE/g ve 12.11±0. değeri ile yapraklarda gözlenmiştir. DPPH, ABTS, Toplam antioksidan kapasite, FRAP ve Fenantrolin aktivitesi için sırasıyla 81 µg /mL. Yaprakların LC-MS/MS analizi, meyveler ana bileşik olarak hesperidin (311.70 mg/g) içerirken, ana bileşik olan rutinin (24392.59 mg/g) varlığını ortaya koymaktadır.

Keywords

• Zizyphus lotus
• Toplam fenolik
• Antioksidan potansiyeli
• LC–MS/MS analizi

LC–MS/MS Profiling of Algerian Zizyphus lotus Extracts and their in vitro Antioxidant Activity: A Comparative Study

Abstract

In this study, it was aimed to determine antioxidant activities and chemical composition of leaf and fruit extracts of Zizyphus lotus which has been used traditionally in folk medicine of Algeria. Total phenolic content (TPC) and total flavonoid content (TFC) were determined by the colorimetric assay, respectively. The extracts were analyzed by LC–MS/MS. Furthermore, the antioxidant potential of various extracts was evaluated using different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS), and Phenanthroline activity using UV spectrophotometer. The quantitative analysis of polyphenols and flavonoids content showed that the leaves have the highest amount (279.07±18.84 mg GAE/g E and 132.74±27.74 mg ER/g E, respectively). The highest antioxidant activity was observed in the leaves with the value of 24.10±3.49 µg/mL, 9.365 ± 0.532 µg/mL, 0.19±0.093, 0.636±0.033 mg AAE/g, and 12.11±0.81 µg/mL for DPPH, ABTS, total antioxidant capacity, FRAP and Phenanthroline activity, respectively. The LC–MS/MS analysis of leaves reveals the presence the major compound, rutin (24392.59 mg/g), when fruits contain hesperidin (311.70 mg/g) as a major compound.

Keywords

• Zizyphus lotus
• Total phenolic
• Antioxidant potential
• LC–MS/MS analysis

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