An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

Ümit Kumbıçak; Zübeyde Kumbıçak

doi:10.31594/commagene.1928136

EN TR

An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

Abstract

Chelicerates constitute a broad group of arthropods that include scorpions, mites, spiders, horseshoe crabs, sea spiders, and ticks. Among them, spiders are a species-rich order that has adapted to terrestrial in the world. In this study, it was aimed to determine the cytological characteristics of Haplodrassus ovtchinnikovi Ponomarev, 2008, a species belonging to the family Gnaphosidae. The specimens used in the study were collected from the central district of Nevşehir province between March and May 2024. In this study, where actively dividing tissues were used, the standard plate spreading preparation method was applied. For this purpose, gonads dissected from specimens were treated respectively with hypotonic solution, fixatives, and acetic acid solution, followed by preparation of spreading slides. Preparations with best chromosome spreads in mitotic and meiotic stages were selected for staining. The preparations were examined under the microscope, and metaphase stages for the karyotype, as well as meiotic division stages for division behavior, were identified and photographed. Karyotype results revealed that the species has a diploid chromosome number and sex chromosome system of 2n♂=22(X1X2). A gradual decrease in chromosome lengths was observed. Considering the cytogenetic characteristics of previously studied species within the family Gnaphosidae, the meiotic stages showed a high degree of conserved features. In particular, the sex chromosomes were observed to stain more intensely during meiosis I, while in meiosis II they stained at levels equivalent to the autosomes. Additionally, the species was found to conform to the chiasmatic meiosis model.

Keywords

Ethical Statement

Ethics committee approval is not required for this study.

Keliserlilerde Kromozomal Çalışmaya Bir Örnek: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

Abstract

Keliserliler; akrepleri, akarları, örümcekleri, at nalı yengeçleri, deniz örümcekleri ve keneleri kapsayan, eklembacaklıların geniş bir grubunu oluşturur. Bunlardan örümcekler, dünyanın hemen her ekosistemine uyum sağlamış ve tür sayısı bakımından zengin bir takımdır. Bu çalışmada Gnaphosidae familyasına ait Haplodrassus ovtchinnikovi Ponomarev, 2008 türünün sitolojik karakteristiklerinin çıkarılması amaçlanmıştır. Çalışmada kullanılan örnekler Mart-Mayıs (2024) tarihleri arasında Nevşehir ili merkezden toplanmıştır. Aktif bölünen dokuların kullanıldığı çalışmada standart kromozom yayma preparasyon yöntemi uygulanmıştır. Bunun için diseksiyonla çıkarılan gonadlar, hiponik çözelti, fiksatifler ve asetik asit çözelti serilerinde tutulmuş ve son aşamada yayma preparatı hazırlanmıştır. Mitoz ve mayoz bölünme evrelerinin en iyi yayılma gösterdiği preparatlar seçilerek boyanmıştır. Preparatlar mikroskopta incelenerek karyotip için metafaz evreleri ve bölünme davranışları için de mayoz bölünme evreleri tespit edilerek fotoğraflanmıştır. Karyotip sonuçları, türün diploid kromozom sayısı ve eşey sistemi modelini 2n♂=22 (X1X2) şeklinde ortaya koymuştur. Kromozomların uzunluklarında sıralı bir azalış dikkati çekmiştir. Mayoz bölünme evrelerinde ise Gnaphosidae familyasında çalışılan türlerin sitogenetik özellikleri dikkate alındığında yüksek düzeyde korunan özelliklerin benzer olduğu görülmüştür. Özellikle mayoz I evrelerinde eşey kromozomlarının daha koyu boyandıkları ve mayoz II evrelerinde ise otozomlarla eşit seviyede boyandıkları tespit edilmiştir. Ayrıca türün kiyazmatik mayoz modeline uygun olduğu da belirlenmiştir.

Keywords

Ethical Statement

Bu çalışma için etik kurul onayı gerekmemektedir.

References

Araujo, D., Schneider, M.C., Paula-Neto, E. & Cella, D.M. (2026). The spider cytogenetic database,” Version 14, 2026. Retrieved from www.arthropodacytogenetics.bio.br/spiderdatabase
Araujo, D., Schneider, M.C., Paula-Neto, E. & Cella, M.D. (2012). Sex Chromosomes and Meiosis in Spiders: A Review. In: Swan A. (ed.), Meiosis-Moleculer Mechanisms and Cytogenetic Diversity. In Tech, Rijeka, Croatica, 87-109. https://doi.org/10.5772/31612
Danışman, T., Kunt, K.B., Özkütük, R.S. & Coşar, İ. “The Checklist of the Spiders of Turkey” Version 2025. Retrieved from http://www.spidersofturkey.info.
Gorlova, O.Yu., Gorlov, I.P., Nevo, E. & Logunov, D.V. (1997). Cytogenetic studies on seventeen spider species from Israel. Bulletin of the British Arachnological Society, 10 (7), 249–252.
Hackman, W. (1948). Chromosomenstudien an Araneen mit besonderer berücksichtigung der geschlechtschromosomen. Acta Zoologica Fennica, 54, 1-101.
Kara, C. (2025). İtulumaz dağları (Niğde) örümcek faunası. Retrieved from https://tez.yok.gov.tr/UlusalTezMerkezi/
Kumbıçak, Z., Ergene, S., Karataş, A., & Kumbıçak, Ü. (2011). Cytogenetic studies on five species of spiders from Turkey (Araneae: Gnaphosidae, Lycosidae). Journal of Arachnology, 39(3), 490-494. https://doi.org/10.1636/B11-06.1
Pekàr, S. & Kràl, J. (2001). A comparative study of the biology and karyotypes of two central European zodariid spiders (Araneae, Zodariidae). The Journal of Arachnology, 29(3), 345-353. https://doi.org/10.1636/0161-8202(2001)029[0345:ACSOTB]2.0.CO;2

Details

Primary Language

English

Subjects

Genetics (Other)

Journal Section

Research Article

Authors

Ümit Kumbıçak ^*
0000-0002-1294-3706
Türkiye

Zübeyde Kumbıçak
0000-0001-5949-1092
Türkiye

Publication Date

July 14, 2026

Submission Date

April 11, 2026

Acceptance Date

July 7, 2026

Published in Issue

Year 2026 Volume: 10 Number: 1

DOI

https://doi.org/10.31594/commagene.1928136

IZ

https://izlik.org/JA29MP26AZ

Cite

RIS / Bibtex

APA

Kumbıçak, Ü., & Kumbıçak, Z. (2026). An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae). Commagene Journal of Biology, 10(1). https://doi.org/10.31594/commagene.1928136

AMA

1.Kumbıçak Ü, Kumbıçak Z. An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae). Comm. J. Biol. 2026;10(1). doi:10.31594/commagene.1928136

Chicago

Kumbıçak, Ümit, and Zübeyde Kumbıçak. 2026. “An Example of Chromosomal Study in Chelicerates: Haplodrassus Ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)”. Commagene Journal of Biology 10 (1). https://doi.org/10.31594/commagene.1928136.

EndNote

Kumbıçak Ü, Kumbıçak Z (July 1, 2026) An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae). Commagene Journal of Biology 10 1

IEEE

[1]Ü. Kumbıçak and Z. Kumbıçak, “An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)”, Comm. J. Biol., vol. 10, no. 1, July 2026, doi: 10.31594/commagene.1928136.

ISNAD

Kumbıçak, Ümit - Kumbıçak, Zübeyde. “An Example of Chromosomal Study in Chelicerates: Haplodrassus Ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)”. Commagene Journal of Biology 10/1 (July 1, 2026). https://doi.org/10.31594/commagene.1928136.

JAMA

1.Kumbıçak Ü, Kumbıçak Z. An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae). Comm. J. Biol. 2026;10. doi:10.31594/commagene.1928136.

MLA

Kumbıçak, Ümit, and Zübeyde Kumbıçak. “An Example of Chromosomal Study in Chelicerates: Haplodrassus Ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)”. Commagene Journal of Biology, vol. 10, no. 1, July 2026, doi:10.31594/commagene.1928136.

Vancouver

1.Ümit Kumbıçak, Zübeyde Kumbıçak. An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae). Comm. J. Biol. 2026 Jul. 1;10(1). doi:10.31594/commagene.1928136

General Information

Commagene Journal of Biology accepts unpublished original research from all areas of biology as a research article, review articles, case reports, short communications, and etc. Review articles may be accepted after they have been evaluated by the editors in limited numbers in each year.

Articles submitted to the journal will be checked against plagiarism with the help of any plagiarism software and the report should be submitted with the manuscript. Similarity rate should be less than 25% in total and less than 5% from 1 reference. Papers with any type of plagiarism will be rejected automatically, and the authors will lose the right to submit and publish the manuscript to our journal. The editors reserve the right to move manuscripts from one category to another.

Manuscripts may be rejected without peer review if they do not comply with the instructions to authors or are beyond the aim and scope of the journal. All manuscripts should be sent with the authors' contribution form, cover, and copyright agreement letter signed by all the authors. Manuscripts sent without the cover letter are not processed.

AI USE POLICY

USE OF ARTIFICIAL INTELLIGENCE (AI) TOOLS

The journal permits the use of artificial intelligence (AI)–assisted technologies (including large language models, machine learning tools, and image-generation systems) in manuscript preparation, subject to the following conditions:

1. Disclosure and Transparency

Authors must disclose the use of any AI tools in the preparation of their manuscript. This disclosure must specify the name of the tool, its version (if applicable), and the purpose for which it was used. Disclosures should be included in a dedicated “AI Use Declaration” section and, where relevant, within the Methods section.

2. Authorship and Accountability

AI tools do not meet the criteria for authorship and must not be listed as authors. All authors retain full responsibility and accountability for the accuracy, integrity, and originality of the work, including any content generated or assisted by AI tools.

3. Scientific Integrity and Reproducibility

AI tools must not be used to generate, manipulate, or fabricate data, results, or images in a misleading manner. Any use of AI in data analysis, modeling, or image processing must be transparently described in sufficient detail to ensure reproducibility.

4. Originality, Plagiarism, and Copyright Compliance

Authors are responsible for ensuring that AI-assisted content does not constitute plagiarism or infringe upon copyright. All sources must be appropriately cited, and reused materials must comply with licensing requirements.

5. Use in Figures, Images, and Graphical Content

The use of AI tools to generate or modify images, figures, or graphical abstracts must be explicitly disclosed. Such content must not misrepresent original data or experimental findings. The journal reserves the right to request original, unprocessed data or images.

6. Confidentiality and Data Protection

Authors must not upload confidential, proprietary, or unpublished data to AI tools that do not provide adequate guarantees of data privacy and security. This includes manuscripts under peer review.

7. Editorial and Peer Review Oversight

The journal may use tools to assess AI-generated content and reserves the right to request additional information regarding AI use. Manuscripts may be rejected if AI use is not properly disclosed or is deemed inappropriate.

AI USE DECLARATION

Authors are required to include a statement regarding the use of artificial intelligence (AI) tools in the preparation of their manuscript. This statement must appear in a separate section titled “AI Use Declaration” prior to the References.

Authors should use one of the following formats:

No Use of AI

The authors declare that no artificial intelligence (AI) tools were used in the preparation of this manuscript.

Use of AI for Language or Writing Assistance

The authors declare that artificial intelligence (AI) tools were used for language editing and/or writing assistance during the preparation of this manuscript. Specifically, [tool name, e.g., ChatGPT] was used for [brief description of use]. The authors have reviewed and approved the final version of the manuscript and take full responsibility for its content.

Use of AI in Data Analysis or Research Process

The authors declare that artificial intelligence (AI) tools were used in this study. [Tool name and version] was used for [specific purpose, e.g., data analysis, modeling, or image processing]. Details of this use are provided in the Methods section. The authors take full responsibility for the accuracy and integrity of the results.

Preparation of the Manuscript

All of the manuscripts, including short notes, must contain at least 4-6 keywords, not included in the title of the Manuscript.

The Structure of the Manuscript

1. Title page (title, author's full name, author's affiliation, corresponding author) should be uploaded as a separate file from the main MS

2. Abstract page (Title, Abstract, keywords)

3. The main text of the manuscript (Introduction, Materials and Methods, Results, Discussion)

4. Acknowledgments

5. References

6. Table and figure captions following the tables and figures.

Abstract: All types of manuscripts, including short notes, must contain an abstract of a minimum 50 and a maximum of 250 words and should summarize the essential results and conclusions.

Manuscript, figures, and tables must be submitted as an electronic version only by using the Turkish JournalPark Submission System.

Manuscript text should be processed with MS-Word (OpenOffice / Libre Office can be used, but in this case, the file should be saved as .docx), typed throughout in letter quality with font size 12, Times New Roman, on A4 (210 x 297mm) paper, with margins of at least 25mm on each side. Pages should be numbered consecutively in the bottom, right-hand corner. The article should not exceed 25 pages with all headings, tables, figures, and references.

For all types of papers, the title page must contain: the title of the paper; name(s) of the author(s) and affiliations, ORCID ID, and the name and address of the corresponding author with contact information. The title page has to be uploaded as a separate page to the Dergipark system.

The tables and figures should be given on a separate page after the references.

Turkish and English manuscripts are acceptable. The abstract must be written in both languages. Turkish abstracts will be written by language editors of the journal if needed by foreign authors.

APA style should be followed in the writing of references.

Avoid quoting unpublished reports or theses.

References should be ordered alphabetically at the end of the manuscript.

All illustrations, including photos and charts, must be labeled as Figures and should be cited in the relevant section.

Tables must be sent both in the Word file and as separate Excel files.

Authors may suggest 5 scholars (with their names, affiliations, and e-mail addresses) in their relevant fields, at least two of whom should be from another country than the country of origin of the authors. Editors may not prefer recommended referees.

The authors have to edit the article according to the pre-check file before uploading the article to the system. Articles that are not prepared according to the journal rules are rejected without being evaluated.

Keywords: It must contain 4-6 keywords that are not included in the title of the Manuscript.

Introduction: It must be a concise description of the background, rationale, aims, and specific objectives of the paper.

Materials and methods: This section should provide enough information to permit the repetition of the experiment and/or fieldwork. The technical description of study methods should be given only if such methods are new; otherwise, a short presentation is enough.

The Results: This section must be a concise presentation of the finding of the study. Avoid the presentation of the same information as text, and/or figure, and/or a table.

Discussion: This section should deal with the significance of the results and their relationship to the aims of the paper. Also include how the findings of the paper will change and influence the state of our knowledge about the subject. It may be combined with the results section if necessary.

Short Notes: This type of paper is generally less than three to four manuscript pages. It should be produced as continuous text, preceded by an abstract of no more than 180 words.

Editor in Chief

Mehmet Zülfü YILDIZ

Ecology, Terrestrial Ecology, Population Ecology, Freshwater Ecology, Biogeography and Phylogeography, Phylogeny and Comparative Analysis, Animal Systematics and Taxonomy, Life Histories, Vertebrate Biology, Conservation and Biodiversity

Managing Editor

Bahadır AKMAN

Terrestrial Ecology, Animal Systematics and Taxonomy, Animal Behaviour

Technical Editor

Bahadır AKMAN

Terrestrial Ecology, Animal Systematics and Taxonomy, Animal Behaviour

Language Editor (English)

Nazmiye GÜREL CENNETKUŞU

Linguistics, Other Fields of Education (Other)

Language Editor (Turkish)

Mehmet Malik BANKIR

Turkish Language and Literature, New Turkish Language (Turkish of Old Anatolia, Ottoman, Turkiye)

Süleyman AYDENIZ

Dialectology, Turkish Language and Literature, New Turkish Language (Turkish of Old Anatolia, Ottoman, Turkiye), Education, Turkish and Social Sciences Education

Statistics Editor

İsmail YILDIZ

Information Systems Organisation and Management, Management Information Systems, Data Structures and Algorithms, Data Management and Data Science, Data Mining and Knowledge Discovery, Bioinformatics and Computational Biology, Quality Assurance in Higher Education, Tourism Policy, Biostatistics, Statistical Quality Control, Health Informatics and Information Systems, Health Management, Medical Education, Quality Management, Genetically Modified Animals, Animal Science, Genetics and Biostatistics

Layout Editor

Turgay KARATAŞ

Computer Aided Design in Visual Communication, Virtual and Mixed Reality, Digitalization, Semiotics, Design, Painting

Section Editors

Konrad MEBERT

Biological Sciences, Ecology, Animal Systematics and Taxonomy, Animal Physiology-Ecophysiology, Animal Cell and Molecular Biology

Nihal DOĞRUÖZ GÜNGÖR

Bacteriology, Microbial Ecology, Microbiology (Other)

Ebru GÖNCÜ

Biological Sciences, Cell Development, Proliferation and Death, Cell Metabolism, Animal Physiology-Ecophysiology, Animal Physiology - Cell, Animal Cell and Molecular Biology

Ahmad ALI

Plant Physiology, Biochemistry and Cell Biology, Enzymes, Proteomics and Intermolecular Interactions, Industrial Biotechnology, Nutrition and Dietetics, Medical Biochemistry and Metabolomics, Medicinal and Biomolecular Chemistry, Food Sciences

Tarkan YORULMAZ

Terrestrial Ecology, Population Ecology

Hakan KARAARDIC

Biological Sciences, Ecology, Ornithology, Biology Education, Science Education

Ertan YOLOĞLU

Biological Sciences, Biochemistry and Cell Biology, Ecology, Animal Physiology-Ecophysiology

Mehdi YOUNESSI-HAMZEKHANLU

Plant Physiology

Ali ALAŞ

Ecology, Hydrobiology, Parasitology, Science and Mathematics Education, Limnology

Ali SATAR

Entomology

Mustafa TEKPINAR

Bioinformatic Methods Development, Bioinformatics and Computational Biology (Other), Atomic and Molecular Physics, Biophysics, Computational Chemistry

Mehmet BOĞA

Pharmacology and Pharmaceutical Sciences, Chemical Sciences, Health Sciences

Suleyman ILHAN

Biological Sciences, Biochemistry and Cell Biology (Other)

Esen POYRAZ TINARTAŞ

Cell Development, Proliferation and Death, Cell Metabolism, Animal Physiology-Ecophysiology

Feyza CANDAN

Ecology, Plant and Fungus Systematics and Taxonomy

Azhar AL-KHAZALI

Freshwater Ecology, Animal Systematics and Taxonomy, Animal Behaviour, Invertebrate Biology

Secretariat

Fatma ÜÇEŞ

Biological Sciences, Population Ecology, Vertebrate Biology

Advisory Board

Faraham AHMEDZADEH

Terrestrial Ecology, Molecular Ecology

Ersen Aydın YAĞMUR

Animal Systematics and Taxonomy

Görkem DENIZ SÖNMEZ

Plant Biochemistry, Plant Cell and Molecular Biology, Genomics and Transcriptomics, Proteomics and Metabolomics, Sequence Analysis, Bioprocessing, Bioproduction and Bioproducts, Fermentation, Phylogeny and Comparative Analysis, Gene Expression, Genome Structure and Regulation, Mathematical Sciences

Deniz ERGÜDEN

Hydrobiology, Animal Systematics and Taxonomy

Sibel ALAGÖZ ERGÜDEN

Marine and Estuarine Ecology, Hydrobiology, Invasion Ecology

Selim KARAHAN

Botany (Other), Medical Pharmacology, Clinical Sciences (Other), Psychopharmacology

AA

Asriyana ASRIYANA

Aquatic Toxicology , Wildlife and Habitat Management, Aquaculture and Fisheries Stock Assessment

GG

Gözde GÜRELLI

Parasitology

Osman SEYYAR

Animal Systematics and Taxonomy, Entomology, Invertebrate Biology

Mustafa AKKUŞ

Fisheries Management, Fisheries Technologies

EB

Eyup BAŞKALE

Biological Sciences, Terrestrial Ecology

DC

Diana CUPŞA

Hydrobiology, Invasion Ecology, Animal Systematics and Taxonomy, Limnology

EE

Esra EROĞLU ÖZKAN

Pharmaceutical Sciences

Hikmet Sami YILDIRIMHAN

Host-Parasite Interactions, Parasitology

Shahin GAVANJI

Industrial Microbiology, Pharmaceutical Biotechnology

Fatma ÜÇEŞ

Biological Sciences, Population Ecology, Vertebrate Biology

SK

Said KEWEDAR

Biochemistry and Cell Biology

Kerim ÇIÇEK

Biological Sciences, Ecology, Terrestrial Ecology, Population Ecology, Community Ecology, Animal Systematics and Taxonomy, Wildlife and Habitat Management

	Scopus
	CrossRef
	idealonline
	TR Dizin

An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

An Example of Chromosomal Study in Chelicerates: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

Abstract

Keywords

Ethical Statement

Keliserlilerde Kromozomal Çalışmaya Bir Örnek: Haplodrassus ovtchinnikovi Ponomarev, 2008 (Gnaphosidae: Araneae)

Abstract

Keywords

Ethical Statement

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite

Aim & Scope

Author Guidelines

Ethical Principles and Publication Policy

Price Policy

Indexes

Citation Indexes

Journal Boards

Editor in Chief

Managing Editor

Technical Editor

Language Editor (English)

Language Editor (Turkish)

Statistics Editor

Layout Editor

Section Editors

Secretariat

Advisory Board