Some Chemical and Biological Properties of Verbascum tripolitanum Growing in Hatay

Abstract

In this study, antioxidant and antimicrobial properties and fatty acid compositions of Verbascum tripolitanum were determined. Total phenolic contents were determined by Folin-Ciocaltaeu method. Antioxidant activity was tested with DPPH free radical scavenging assay, ferric ion reducing power (FRAP), cupric reducing antioxidant capacity (CUPRAC), and β-carotene-linoleic acid emulsion method. The antimicrobial effects of the V. tripolitanum were tested by the microdilution broth method against two gram-positive bacterias (Staphylococcus aureus, Methicillin-resistant S. aureus), two gram-negative bacterias (Pseudomonas aeruginosa, Escherichia coli), and one kind of yeast (Candida albicans). The fatty acid compositions were determined by GC-FID.

Keywords

• Verbascum tripolitanum,antioxidant,antimicrobial,fatty acid

Hatay’da Yetişen Verbascum tripolitanum’un Bazı Kimyasal ve Biyolojik Özellikleri

Abstract

Bu çalışmada, Hatay bölgesinde yetişen Verbascum tripolitanum’un antioksidan ve antimikrobiyal özellikleri, yağ asitleri içeriği belirlenmiştir. Toplam fenolik madde miktarı Folin-Ciocaltaeu yöntemi ile tayin edilmiştir. Antioksidan aktivite DPPH serbest radikal süpürme yöntemi, Fe⁺³ indirgeme metodu (FRAP), bakır iyonu indirgeme metodu (CUPRAC), ve β-karoten-linoleik asit emülsiyon yöntemleri ile test edilmiştir. V. tripolitanum’un antimikrobiyal etkisi 2 gram pozitif bakteri (Staphylococcus aureus, metisiline dirençli S. aureus), 2 gram negatif bakteri (Pseudomonas aeruginosa, Escherichia coli) ve 1 maya türüne (Candida albicans) karşı mikrodilüsyon broth metodu ile analiz edilmiştir. Yağ asitleri analizi için GC-FID kullanılmıştır.

Keywords

• Verbascum tripolitanum,antioksidan,antimikrobiyal,yağ asitleri

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