Hatay’da Yetişen Verbascum tripolitanum’un Bazı Kimyasal ve Biyolojik Özellikleri

Yıl 2019, , 79 - 82, 20.12.2019

Hatice Danahaliloğlu , Yener Tekeli Yelda Güzel

https://doi.org/10.31594/commagene.598450

Öz

Bu çalışmada,
Hatay bölgesinde yetişen Verbascum tripolitanum’un antioksidan ve antimikrobiyal
özellikleri, yağ asitleri içeriği belirlenmiştir. Toplam fenolik madde miktarı Folin-Ciocaltaeu
yöntemi ile tayin edilmiştir. Antioksidan aktivite DPPH serbest radikal süpürme
yöntemi, Fe⁺³ indirgeme metodu (FRAP), bakır iyonu indirgeme metodu (CUPRAC),
ve β-karoten-linoleik asit emülsiyon yöntemleri ile test edilmiştir. V. tripolitanum’un antimikrobiyal etkisi 2 gram pozitif
bakteri (Staphylococcus aureus, metisiline dirençli S. aureus), 2 gram negatif bakteri (Pseudomonas aeruginosa, Escherichia coli) ve 1 maya türüne (Candida
albicans) karşı mikrodilüsyon broth metodu ile analiz edilmiştir. Yağ asitleri
analizi için GC-FID kullanılmıştır.

Anahtar Kelimeler

Verbascum tripolitanum, antioksidan, antimikrobiyal, yağ asitleri

Destekleyen Kurum

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

243

Teşekkür

Bu çalışmaya destek olan Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Birimine teşekkür ederiz (Proje no: 243).

Some Chemical and Biological Properties of Verbascum tripolitanum Growing in Hatay

Öz

In this study, antioxidant
and antimicrobial properties and fatty acid compositions of Verbascum tripolitanum
were determined. Total
phenolic contents were determined by Folin-Ciocaltaeu method. Antioxidant activity
was tested with DPPH free radical scavenging assay, ferric ion reducing power (FRAP),
cupric reducing antioxidant capacity (CUPRAC), and β-carotene-linoleic acid emulsion
method. The antimicrobial effects of the V. tripolitanum were tested
by the microdilution broth method against two gram-positive bacterias (Staphylococcus
aureus, Methicillin-resistant S. aureus), two gram-negative bacterias
(Pseudomonas aeruginosa, Escherichia coli), and one kind of yeast
(Candida albicans). The fatty acid compositions were determined by GC-FID.

Anahtar Kelimeler

Verbascum tripolitanum, antioxidant, antimicrobial, fatty acid

Proje Numarası

243

Kaynakça

• Benli, M., Güney, K., Bingöl, Ü., Geven, F., & Yiğit, N. (2007). Antimicrobial activity of some endemic plant species from Turkey. African Journal of Biotechnology, 6(15), 1774-1778.
• Blainski, A., Lopes, G.C., & Mello, J.C.P. (2013). Application and analysis of the Folin Ciocalteu Method for the determination of the total phenolic content from Limonium brasiliense L. Molecules, 18, 6852-6865.
• Brand-Williams, W., Cuvelier, M.E., & Berset, C.L.W.T. (1995). Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28(1), 25-30.
• Dalar, A., & Konczak, I. (2012). Botanicals from Eastern Anatolia Region of Turkey: Antioxidant capacity and phenolic constituents of endemic herbal medicines. Journal of Herbal Medicine, 2, 126–135.
• Faydaoğlu, E., & Sürücüoğlu, M.S. (2013). Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidan aktiviteleri ve kullanım olanakları. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(2), 233-265.
• Güçlü, K., Altun, M., Özyürek, M., Karademir, S.E., & Apak, R. (2006). Antioxydant capacity of fresh, sun- and sulphited-dried Malatya apricot (Prunus armeniaca) assayed by CUPRAC, ABTS/TEAC and folin methods. International Journal of Food Science and Technology, 41, 76-85.
• Gülçin, İ. (2005). The antioxidant and radical scavenging activities of black pepper (pipernigrum) seeds. International Journal of Food Sciences and Nutrition 56(7) 491-499.
• Gülçin, İ., Köksal, E., Elmastaş, M., & Aboul-Enein, H.Y. (2007). Determination of in vitro antioxidant and radical scavenging activity of Verbascum oreophilum C. Koch Var. Joannis (Fam. Scrophulariaceae). Research Journal of Biological Sciences, 2(3), 372-382.
• Hou, W.C., Lin, R.D., Cheng, K.T., Hung, Y.T., Cho, C.H., Chen, C.H., Hwang, S.Y., & Lee, M.H. (2003). Free radical scavenging activity of Taiwanese native plants. Phytomedicine, 10, 170–175.
• Kahkönen, M., Hopia A., Vuorela H.J., Rauha J.P., Pihlaja, K., Kujala, T.S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47, 3954−3962.
• Kahraman, Ç., Ekizoğlu, M., Kart, D., Akdemir, Z.Ş., & Tatlı, İ. (2011). Antimicrobial activity of some Verbascum species growing in Turkey. FABAD Journal of Pharmaceutical Sciences, 36, 11-15.
• Kang, C.H., Hong, C.R., Youm, H.S., Choi, S.I., & Heo, T.R. (2008). Growth ınhibitory activities of siegesbeckiae glabrescens against foodborne pathogens. Journal of Biotechnology, 136(1), 717-742.
• Kaur, C., & Kapoor, H.C. (2002). Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science and Technology, 37, 153-161.
• Kozan, E., Çankaya, İ.T., Kahraman, Ç., Akkol, E.K., & Akdemir, Z. (2011). The in vivo anthelmintic efficacy of some Verbascum species growing in Turkey. Experimental Parasitology, 129, 211–214.
• Kumar, G.P., & Singh, S.B. (2011). Antibacterial and antioxidant activities of ethanol extracts from Trans Himalayan medicinal plants. European Journal of Applied Sciences, 3(2), 53-57.
• Küpeli, E., Tatlı, İ., Akdemir, Z., & Yeşilada, E. (2007). Bioassay-guided ısolation of anti-inflammatory and antinociceptive glycoterpenoids from the flowers of Verbascum lasianthum Boiss. ex Bentham. Journal of Ethnopharmacology, 110, 444–450.
• Mothana, R.A., Abdo, S.A., Hasson, S., Althawab, F., Alaghbari, S.A., & Lindequist, U. (2010). Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some Yemeni medicinal plants. Evidence-based Complementary and Alternative Medicine, 7(3), 323-330.
• Oyaizu, M. (1986). Studies on product of browning reaction preparedfrom glucose amine. Japanese Journal of Nutrition, 44, 307.
• Özcan, B., Esen, M., & Çalışkan, M. (2011). Antimicrobial and antioxidant activities of the various extracts of Verbascum pinetorum Boiss. O. Kuntze (Scrophulariaceae). European Review for Medical and Pharmacological Sciences., 15(8), 900-905.
• Sarıkürkcü, C., Zengin, G., Aktümsek, A., Ceylan, O., & Şanda, M.A. (2015). Onopordum Anatolicum tohumlarının antioksidan aktiviteleri. Selçuk Üniversitesi Fen Fakültesi Dergisi, 41, 89-96.
• Tatlı, İ.İ., & Akdemir, Z. (2004). Chemical constituents of Verbascum L. Species. FABAD Journal of Pharmaceutical Sciences, 29, 93-107.
• Yamaguchi, T., Takamura, H., Matoba, T., & Terao, J. (1998). HPLC method for evaluation of the free radical-scavenging activity of foods by using 1, 1-diphenyl-2-picrylhydrazyl. Bioscience, Biotechnology, and Biochemistry, 62(6),1201-1204.