Penkonazole Maruz Bırakılan Tatlı Su Midyelerinin Solungaç Na+/K+-ATPaz, Mg2+-ATPaz, Ca2+-ATPaz ve Total-ATPaz Aktivitelerinin Değerlendirilmesi

Year 2019, , 88 - 92, 20.12.2019

Ertan Yoloğlu

https://doi.org/10.31594/commagene.632082

Cited By: 3

Abstract

Triazol bir fungusit olan penkonazol, tarımsal üretimi
arttırmak ve fungusları kontrol etmek için dünya çapında yaygın olarak
kullanılmaktadır. Çift kabuklu yumuşakçalar, özellikle midyeler, sucul
ekosistemlerde kirliliğin spesifik biyomonitor organizmalarıdır. Bu çalışmada
tatlı su midyelerinin (Unio mancus)
solungaç Na⁺/K⁺-ATPaz, Mg²⁺-ATPaz, Ca²⁺-ATPaz
ve Total-ATPaz aktiviteleri üzerine penkonazol maruziyetinin olası toksik
etkileri değerlendirilmiştir. Bu amaçla, 96 saatlik statik yenileme test
sisteminde tatlı su midyeleri farklı konsantrasyonlarda penkonazole (1, 10, 100
ve 1000 µg AI L^-1) maruz bırakılmıştır. Toksisite testlerinde
penkonazolün ticari formu (TOPAS^® 100 EC, Syngenta, Almanya)
kullanılmıştır. Maruziyet ortamındaki gerçek penkonazol konsantrasyonları
LC-MS/MS analizi ile belirlenmiştir. LC-MS/MS analizi sonuçlarına göre,
maruziyet ortamındaki gerçek penkonazol konsantrasyonlarının, nominal
penkonazol konsantrasyonlarından yaklaşık % 25 oranında daha düşük olduğu
belirlenmiştir. 96 saatlik maruziyetten sonra, en yüksek penkonazol
konsantrasyonu Mg²⁺-ATPaz hariç diğer ATPaz aktivitelerinin önemli
düzeyde inhibisyonuna neden olmuştur (P < 0.05). Elde edilen veriler, akut
penconazol maruziyetinin tatlı su midyeleri için potansiyel bir risk
olabileceğini ve ATPaz enzimlerinin akuatik toksikoloji çalışmaları için önemli
ve faydalı biyokimyasal belirteçler olduğunu göstermiştir.

Keywords

Penkonazol toksisitesi, Tatlı su midyesi, ATPazlar, LC-MS/MS

Assessment of Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and Total-ATPase Activities in Gills of Freshwater Mussels Exposed to Penconazole

Abstract

Penconazole,
a triazole fungicide, is widely used worldwide to increase agricultural
production and to control fungus. Bivalves, especially mussels, are specific
biomonitor organisms of pollution in aquatic ecosystems. This study deals with
the possible toxic effects of exposure to penconazole on Na⁺/K⁺-ATPase,
Mg²⁺-ATPase, Ca²⁺-ATPase, and Total-ATPase activities in
the gills of freshwater mussels (Unio
mancus). In this context, freshwater mussels were exposed to different
concentrations of penconazole (1, 10, 100, and 1000 µg AI L^-1) in 96
hours static-renewal test system. The commercial form of penconazole (TOPAS^®
100 EC, Syngenta, Germany) was used in toxicity tests. The actual penconazole
concentrations in the exposure media were determined by LC-MS/MS analysis.
According to the LC-MS/MS analysis results, the actual penconazole
concentrations in the exposure media were determined to be approximately 25%
lower than the nominal penconazole concentrations. After 96 hours of exposure,
the highest concentration of penconazole caused significant inhibition of
ATPase activities except Mg²⁺-ATPase activity (P < 0.05). The
observed data indicated that acute penconazole exposure may be a potential risk
for freshwater mussels and that ATPase enzymes are important and useful
biochemical markers for aquatic toxicology studies.

Keywords

Penconazole toxicity, Freshwater mussel, ATPases, LC-MS/MS

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