Lens culinaris'te Sitokinin ve Oksinin Bitki Gelişimi ve Vasküler Dokular Üzerine Etkileri

Abstract

Sitokininler ve oksinlerin Lens culinaris’te daha önce çalışılmasına rağmen bunların vasküler dokular ve bitki gelişimi üzerine olan etkileri hakkında bilgi bulunmamaktadır. Bu çalışmada, bütün mercimek bitkicikleri çeşitli BAP (1 mg/L) ve NAA dozlarında (0.3, 0.6, 0.9, 1.2 mg/L) periyodik 7, 14, 21, 28 ve 35 gün aralıklarında in vitro koşullarda kültüre alınmıştır. Bitki boyu, her bitkideki sürgün sayısı ve köklenmenin rejenerasyon davranışlarının; floem ve ksilem bölgesinin kalınlığının gelişimsel davranışı ve gövde çapının kalınlığı ile bir ilişkisinin olup olmadığı araştırılmıştır. Çoğu durumda, 7 günlük kültür koşulları tüm bitki büyüme düzenleyicileri dozlarında en iyi sonuçları göstermiştir. Yüksek dozdaki BAP ve NAA uygulamaları, uzatılan kültür günü sürelerinde bitki gelişimi ve vasküler dokular üzerinde sağlıksız gelişime neden olmuştur. Floem bölgesinin kalınlığı kontrol grubunda en yüksek olurken, ksilem bölgesinin yalnızca 1 mg/L BAP uygulamasında en yüksek kalınlığa sahip olduğu bulunmuştur. Ayrıca, yüksek BAP ve NAA dozları floem ve ksilem bölgesinde deformasyona ve daralmaya neden olmuştur ve gövde çapında daralmaya yol açmıştır. Bu sonuçlar, uzayan kültür günü sürelerindeki bitki büyüme düzenleyicileri uygulamalarının olumsuz etkilerini ortaya çıkarmaktadır. Vasküler dokular ve diğer dokulardaki hasarı azaltmak, dokulardaki istenen büyüme ve gelişmeyi elde etmek için gün periyodunun tam olarak optimize edilmesi gerektiği sonucuna varılmaktadır.

Keywords

• Doku kültürü,Benzilaminopürin (BAP),Naftalen asetik asit (NAA),floem,ksilem

Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris

Abstract

Cytokinins and auxins were studied in Lens culinaris before; however, there was lack of information about their effects on vascular tissues and their relation with plant development. In this study, intact lentil seedlings were treated with different doses of BAP (1 mg/L) and NAA (0.3, 0.6, 0.9, 1.2 mg/L) with periodic intervals of 7, 14, 21, 28, and 35 days under in vitro conditions. The behaviors of plant height, number of shoots per plant, and rooting were investigated to find if their regeneration behavior is related with the developmental behavior of phloem and xylem thickness and stem radius. In most instances, seven-day culture conditions in all plant growth regulators (PGRs) doses demonstrated the best results. BAP and NAA treatments with high doses on prolonged days of culture caused unhealthy growing on plant development and vascular tissues. While phloem region thickness was the highest in control group, xylem region was found to have the highest thickness in 1 mg/L BAP treatment used singly. Moreover, high doses of BAP and NAA caused deformity and shrinking in phloem and xylem regions that also lead to shrinking in stem radius. These results reveal negative effects of BAP and NAA treatments on prolonged days of culture and conclude that the time period of treatment must be optimized precisely to avoid damages to vascular and other tissues and promote desirable growth and development of tissues.

Keywords

• Tissue culture,Naphthaleneacetic acid (NAA),Benzylaminopurine (BAP),phloem,xylem

Thanks

This study was derived from a M.Sc. thesis of the first author at Department of Agricultural Sciences at Institute of Natural and Applied Sciences, Usak University, Usak, Turkey. The authors would like to express their gratitude to Assoc. Prof. Dr. Ahmet Kahraman from Department of Biology, Faculty of Arts and Sciences, Plant Systematics and Phylogenetics Laboratory (PSPL), Usak University for his constructive comments on the manuscript and excellent technical assistance on anatomical study.

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