Year 2020, Volume 4 , Issue 1, Pages 16 - 21 2020-06-29

Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris
Lens culinaris'te Sitokinin ve Oksinin Bitki Gelişimi ve Vasküler Dokular Üzerine Etkileri

Haydar KÜPLEMEZ [1] , Mehmet Uğur YILDIRIM [2]


Cytokinins and auxins were studied in Lens culinaris before; however, there was lack of information about their effects on vascular tissues and their relation with plant development. In this study, intact lentil seedlings were treated with different doses of BAP (1 mg/L) and NAA (0.3, 0.6, 0.9, 1.2 mg/L) with periodic intervals of 7, 14, 21, 28, and 35 days under in vitro conditions. The behaviors of plant height, number of shoots per plant, and rooting were investigated to find if their regeneration behavior is related with the developmental behavior of phloem and xylem thickness and stem radius. In most instances, seven-day culture conditions in all plant growth regulators (PGRs) doses demonstrated the best results. BAP and NAA treatments with high doses on prolonged days of culture caused unhealthy growing on plant development and vascular tissues. While phloem region thickness was the highest in control group, xylem region was found to have the highest thickness in 1 mg/L BAP treatment used singly. Moreover, high doses of BAP and NAA caused deformity and shrinking in phloem and xylem regions that also lead to shrinking in stem radius. These results reveal negative effects of BAP and NAA treatments on prolonged days of culture and conclude that the time period of treatment must be optimized precisely to avoid damages to vascular and other tissues and promote desirable growth and development of tissues.

Sitokininler ve oksinlerin Lens culinaris’te daha önce çalışılmasına rağmen bunların vasküler dokular ve bitki gelişimi üzerine olan etkileri hakkında bilgi bulunmamaktadır. Bu çalışmada, bütün mercimek bitkicikleri çeşitli BAP (1 mg/L) ve NAA dozlarında (0.3, 0.6, 0.9, 1.2 mg/L) periyodik 7, 14, 21, 28 ve 35 gün aralıklarında in vitro koşullarda kültüre alınmıştır. Bitki boyu, her bitkideki sürgün sayısı ve köklenmenin rejenerasyon davranışlarının; floem ve ksilem bölgesinin kalınlığının gelişimsel davranışı ve gövde çapının kalınlığı ile bir ilişkisinin olup olmadığı araştırılmıştır. Çoğu durumda, 7 günlük kültür koşulları tüm bitki büyüme düzenleyicileri dozlarında en iyi sonuçları göstermiştir. Yüksek dozdaki BAP ve NAA uygulamaları, uzatılan kültür günü sürelerinde bitki gelişimi ve vasküler dokular üzerinde sağlıksız gelişime neden olmuştur. Floem bölgesinin kalınlığı kontrol grubunda en yüksek olurken, ksilem bölgesinin yalnızca 1 mg/L BAP uygulamasında en yüksek kalınlığa sahip olduğu bulunmuştur. Ayrıca, yüksek BAP ve NAA dozları floem ve ksilem bölgesinde deformasyona ve daralmaya neden olmuştur ve gövde çapında daralmaya yol açmıştır. Bu sonuçlar, uzayan kültür günü sürelerindeki bitki büyüme düzenleyicileri uygulamalarının olumsuz etkilerini ortaya çıkarmaktadır. Vasküler dokular ve diğer dokulardaki hasarı azaltmak, dokulardaki istenen büyüme ve gelişmeyi elde etmek için gün periyodunun tam olarak optimize edilmesi gerektiği sonucuna varılmaktadır.

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Primary Language en
Subjects Biology
Journal Section Research Articles
Authors

Orcid: 0000-0003-4094-1318
Author: Haydar KÜPLEMEZ (Primary Author)
Institution: RECEP TAYYIP ERDOGAN UNIVERSITY, FACULTY OF AGRICULTURE AND NATURAL SCIENCES, DEPARTMENT OF FIELD CROPS, FIELD CROPS PR.
Country: Turkey


Orcid: 0000-0002-7419-0682
Author: Mehmet Uğur YILDIRIM
Institution: USAK UNIVERSITY, FACULTY OF AGRICULTURE AND NATURAL SCIENCES, DEPARTMENT OF FIELD CROPS
Country: Turkey


Thanks This study was derived from a M.Sc. thesis of the first author at Department of Agricultural Sciences at Institute of Natural and Applied Sciences, Usak University, Usak, Turkey. The authors would like to express their gratitude to Assoc. Prof. Dr. Ahmet Kahraman from Department of Biology, Faculty of Arts and Sciences, Plant Systematics and Phylogenetics Laboratory (PSPL), Usak University for his constructive comments on the manuscript and excellent technical assistance on anatomical study.
Dates

Application Date : March 16, 2020
Acceptance Date : April 19, 2020
Publication Date : June 29, 2020

Bibtex @research article { commagene704271, journal = {Commagene Journal of Biology}, issn = {}, eissn = {2602-456X}, address = {Adıyaman Bilimsel Araştırmalar Derneği,}, publisher = {ABADER (Adıyaman Bilimsel Araştırmalar Derneği)}, year = {2020}, volume = {4}, pages = {16 - 21}, doi = {10.31594/commagene.704271}, title = {Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris}, key = {cite}, author = {Küplemez, Haydar and Yıldırım, Mehmet Uğur} }
APA Küplemez, H , Yıldırım, M . (2020). Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris . Commagene Journal of Biology , 4 (1) , 16-21 . DOI: 10.31594/commagene.704271
MLA Küplemez, H , Yıldırım, M . "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris" . Commagene Journal of Biology 4 (2020 ): 16-21 <https://dergipark.org.tr/en/pub/commagene/issue/52124/704271>
Chicago Küplemez, H , Yıldırım, M . "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris". Commagene Journal of Biology 4 (2020 ): 16-21
RIS TY - JOUR T1 - Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris AU - Haydar Küplemez , Mehmet Uğur Yıldırım Y1 - 2020 PY - 2020 N1 - doi: 10.31594/commagene.704271 DO - 10.31594/commagene.704271 T2 - Commagene Journal of Biology JF - Journal JO - JOR SP - 16 EP - 21 VL - 4 IS - 1 SN - -2602-456X M3 - doi: 10.31594/commagene.704271 UR - https://doi.org/10.31594/commagene.704271 Y2 - 2020 ER -
EndNote %0 Kommagene Biyoloji Dergisi Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris %A Haydar Küplemez , Mehmet Uğur Yıldırım %T Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris %D 2020 %J Commagene Journal of Biology %P -2602-456X %V 4 %N 1 %R doi: 10.31594/commagene.704271 %U 10.31594/commagene.704271
ISNAD Küplemez, Haydar , Yıldırım, Mehmet Uğur . "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris". Commagene Journal of Biology 4 / 1 (June 2020): 16-21 . https://doi.org/10.31594/commagene.704271
AMA Küplemez H , Yıldırım M . Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Comm. J. Biol.. 2020; 4(1): 16-21.
Vancouver Küplemez H , Yıldırım M . Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology. 2020; 4(1): 16-21.
IEEE H. Küplemez and M. Yıldırım , "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris", Commagene Journal of Biology, vol. 4, no. 1, pp. 16-21, Jun. 2020, doi:10.31594/commagene.704271