Besiyeri Bileşiminin Çayır Salkımotunda (Poa pratensis L. cv. Evora) in vitro Tohum Çimlenmesi ve Bitki Gelişimi Üzerindeki Etkisi

Abstract

Bu çalışmada, Poa pratensis cv. Evora’da De Greef & Jacobs (DG), Linsmaier & Skoog (LS), Murashige & Skoog (MS) ve Schenk & Hildebrandt (SH) besiyerlerinin tohum çimlenmesi ve bitki gelişimi üzerindeki etkileri test edilmiştir. En yüksek çimlenme oranı (%83±2.74) SH ortamında bulunurken, LS ortamı en düşük (%46±4.18) çimlenme oranını vermiştir. Ortalama yaprak sayıları SH (2.10±0.27) ve DG (2.12±0.18) ortamlarından istatistiksel olarak aynı kaydedilmiştir. Ortalama yaprak uzunlukları SH ve DG ortamlarında sırasıyla 4.28±0.28 cm ve 4.16±0.31 cm olarak ölçülmüştür. Maksimum ortalama kök sayısı (3.09±0.26) SH ortamından elde edilmiştir. Bununla birlikte, en düşük ortalama kök sayısı (1.84±0.10) LS ortamında kaydedilmiştir. En uzun kökler (1.43±0.19 cm) SH ortamında yetiştirilen bitkilerde gözlenmiştir. Bununla birlikte, DG ortamı minimum ortalama kök uzunluğunu (0.81±0.08 cm) vermiştir. Sonuç olarak, biyokütle üretimini artırmak için bu tür üzerinde yapılan in vitro doku kültürü çalışmalarında test edilen diğer besiyerlerine kıyasla SH besiyeri tercih edilmelidir. SH ortamının P. pratensis'in in vitro gelişimindeki etkinliği büyüme destekleyicileri kullanılarak artırılabilir.

Keywords

• Makroelementler
• Mikroelementler
• Doku kültürü
• Vitaminler
• Çim

Effect of Medium Composition on in vitro Seed Germination and Plant Development in Kentucky Bluegrass (Poa pratensis L. cv. Evora)

Abstract

In the current study, the effects of De Greef & Jacobs (DG), Linsmaier & Skoog (LS), Murashige & Skoog (MS), and Schenk & Hildebrandt (SH) media were tested on seed germination and plant development in Poa pratensis cv. Evora. The highest germination rate (83±2.74%) was found on SH medium, whereas LS medium gave the lowest (46±4.18%) germination rate. The statistically same leaf numbers were recorded from SH (2.10±0.27) and DG (2.12±0.18) media. SH and DG media gave 4.28±0.28 cm and 4.16±0.31 cm mean leaf lengths, respectively. SH medium gave the maximum mean root number (3.09±0.26). However, the LS medium gave the lowest mean root number (1.84±0.10). The longest roots (1.43±0.19 cm) were observed in the plants grown in SH medium. However, DG medium had the minimum mean root length (0.81±0.08 cm). In conclusion, SH medium should be preferred over the other medium tested in in vitro tissue culture studies on the species to increase the biomass production yield. The efficacy of SH medium in the in vitro propagation of P. pratensis could be increased using growth promoters.

Keywords

• Macroelements
• Microelements
• Tissue culture
• Turfgrass
• Vitamins

Thanks

The author would like to thank Duhan Tırlı and Serdar Yıldız for their help with the inoculation process of the cultures used in this paper.

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