Comparison of Different Life Stages of Total, Phospholipid and Triacylglycerol Fatty Acids of Lucilia sericata

Abstract

Lucilia sericata, which belongs to the Calliphoridae family (Diptera), is used as a debridement tool in open necrotic wounds that do not respond to conventional treatments. Knowing the total, phospholipid (PL), and triacylglycerol (TAG) fatty acid content of L. sericata fly, which is important for health, in its different stages is important both in terms of taxonomy and physiology. After L. sericata samples used in the study were obtained commercially, they were bred under laboratory conditions and then the fatty acids in different stages were analyzed by gas chromatography. Sixteen fatty acids are determined as a result of the analysis. When individual fatty acids are considered, Palmitic acid (16:0), Palmitoleic Acid (16:1n-7), oleic acid (18:1n-9) and Linoleic Acid (18:2n-6) were found to be major ones, while the others are detected in trace amounts. It is determined that, out of total, PL and TAG, saturated fatty acids (SFA) are found at a high percentage in the eggs, monounsaturated fatty acids (MUFA) are the highest in the larvae, and polyunsaturated fatty acids (PUFA) are the highest in fly and pupa. Furthermore, out of total, PL and TAG, ∑PUFA is at a low percentage in the egg and the larval stages. Different results are found at different stages in this study. This may be because fatty acid percentages that change during metamorphosis meet different physiological needs at different phases.

Keywords

• Maggot
• fly
• calliphoridae
• gas chromatograph

Lucilia sericata’nın Total, Fosfolipid ve Triaçilgliserol Yağ Asitlerinin Farklı Yaşam Evrelerinin Karşılaştırılması

Abstract

Calliphoridae ailesindeki (Diptera) olan Lucilia sericata, geleneksel tedavilere yanıt veremeyen açık nekrotik yaralarda debridman aracı olarak kullanılmaktadır. Sağlık açısından önemli olan L. sericata sineğine ait farklı evrelerindeki total, fosfolipit (PL) ve triaçilgliserol (TAG) yağ asit içeriğinin bilinmesi hem taksonomik hem de fizyolojisi açılardan önem taşımaktadır. Çalışmada kullanılan L. sericata örnekleri ticari olarak temin edildikten ve laboratuvar koşullarında üremesi sağlandıktan sonra farklı evrelerindeki yağ asitleri gaz kromatografisi ile analizleri yapılmıştır. Yapılan analiz sonucunda 16 yağ asidi belirlenmiştir. Bireysel yağ asitleri incelendiğinde Palmitik asit (16:0), Palmitoleik Asit (16:1n-7), oleik asit (18:1n-9) ve Linoleik Asit (18:2n-6) majör yağ asitleri olarak belirlenmiştir. Diğerleri eser miktarda tespit edilmiştir. Total, PL ve TAG’de doymuş yağ asitleri (SFA) yumurtada; Tekli Doymamış Yağ Asitleri (MUFA) larvada, Çoklu doymamış yağ asitleri (PUFA) sinek ve pupada yüksek yüzdede olduğu belirlenmiştir. Ayrıca total, PL ve TAG’de yumurta ve larva evresinde ∑PUFA’nın düşük yüzdede olduğu görülmüştür. Çalışmamızda farklı evrelerde farklı sonuçlar tespit edilmiştir. Bunun nedeni metamorfoz süresince değişen yağ asidi yüzdelerinin farklı evrelerindeki farklı fizyolojik ihtiyaçlarının karşılanması şeklinde açıklanabilir.

Keywords

• Magoot
• sinek
• calliphoridae
• gaz kromotografisi

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