Effects of 3-Benzoyl-7-Hydroxy Coumarin Compound on TNF-α and Caspase-3 Immunreactivity in Kidneys of Rats Exposed to Aluminum

Year 2023, Volume: 7 Issue: 1, 73 - 78, 30.06.2023

Ahmet Türk , Tuncay Kuloğlu , Büşra Zencirci , Ali Gürel , Abdullah Karadağ , Yusuf Özay , Serhat Hançer , Muhammed Emre Yüzer , Kenan Türkan , Ahmet Özkaya

https://doi.org/10.31594/commagene.1217426

Cited By: 1

Abstract

In this study, we aimed to investigate the effects of 3-benzoyl-7-hydroxy coumarin compound on apoptotic markers in the kidney tissues of rats treated with Aluminum(Alu). 28 Wistar albino male rats were divided into four groups (n = 7) as: Control Group: 1 ml/kg 0.9% NaCl was administered by oral gavage every other day during the experiment. Aluminium (Alu) Group: Alu was prepared in water and given by intraperitoneal(i.p) for 30 days at an excess of 8.3 mg/kg/day. Treatment Group: 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. Alu + Treatment Group: 8.3 mg/kg Alu was prepared in water and administered i.p for 30 days every other day, and then 10 mg/kg 3-Benzoyl-7-Hydroxy Coumarin was administered to rats by oral gavage every other day for 30 days. At the end of the 30-day experiment, the kidney tissue of the animals were removed under anesthesia and stored under appropriate conditions. In according to our data, Alu administration caused increased Caspase-3 TNF-α and MDA levels in kidney tissue, and 3-benzoyl-7-hydroxy coumarin was found to significantly reverse these changes.

Keywords

Oxidative stress, Apoptosis, Proinflamatuar cytokine, Immunohistochemistry

Supporting Institution

None

Project Number

None

Thanks

Special thanks to Prof. Dr. Murat KOCA (Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Adiyaman University) and Prof. Dr Adnan Kurt (Department of Chemistry, Faculty of Arts and Sciences, Adıyaman University) for their contribution to this study.

Alüminyuma Maruz Kalan Sıçanların Böbreklerinde 3-Benzoil-7-Hidroksi Kumarin Bileşiğinin TNF-α ve Kaspaz-3 İmmün Reaktivitesi Üzerine Etkileri

Abstract

Bu çalışmada, Alüminyum (Alu) ile tedavi edilen sıçanların böbrek dokularında 3-benzoil-7-hidroksi kumarin bileşiğinin apoptotik belirteçler üzerindeki etkilerini araştırmayı amaçladık. Deney için 28 adet Wistar albino erkek ratlar 4 gruba (n=7) ayrıldı; Kontrol Grubu: Deney süresince sıçanlara gün aşırı 1 ml/kg %0.9 NaCl oral gavaj yolu ile verildi. Alüminyum (Alu) Grubu: Suda çözünmüş, Alu bileşiği intraperitonal(i.p.) yol ile 30 gün boyunca 8.3 mg/kg/gün aşırı uygulandı. Tedavi Grubu: Sıçanlara gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj yolu ile 30 gün süreyle uygulandı. Alu + Tedavi Grubu: 8.3 mg/kg Alu su içinde hazırlandı ve i.p. deney süresi boyunca gün aşırı 10 mg/kg 3-Benzoyl-7-Hydroxy kumarin oral gavaj ile uygulandı. 30 günlük deney sonunda anestezi altında hayvanların böbrek dokusu alınarak uygun koşullarda saklandı. Elde edilen verilere göre Alu uygulaması böbrek dokusunda Kaspaz-3, TNF-α ve MDA düzeylerinin artmasına neden olmuş ve 3-benzoil-7-hidroksi kumarin bileşiğinin bu değişiklikleri önemli ölçüde tersine çevirdiği görüldü.

Keywords

Oksidatif stres, Apoptoz, Proinflamatuar sitokin, İmmünohistokimya

Project Number

None

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