Effect of swimming exercise on angiotensin-(1–7)-mediated aortic dilation responses in the nitric oxide synthase inhibition-induced hypertensive rats

Year 2025, Volume: 50 Issue: 4, 1092 - 1102, 22.12.2025

Nur Özen , Leyla Abueid , Ahmet Yıldırım , Pınar Ülker , Filiz Basralı

https://doi.org/10.17826/cumj.1753982

Abstract

Purpose: Regular exercise exerts at least some of its effects on lowering high blood pressure by regulating vascular tone. Angiotensin-(1-7) [Ang-(1-7)], a member of the renin–angiotensin system (RAS), has antihypertensive and vasodilator effects. The aim of this study is to investigate whether the blood pressure-lowering effect of regular exercise is mediated by Ang-(1-7)-induced vasodilation in a nitric oxide synthase (NOS) inhibition-induced hypertension model.
Materials and Methods: 8-week-old Wistar male rats were separated into four groups: Control (C), Hypertension (H), Exercise (E), and Hypertension+Exercise (HE). Rats were subjected to the NOS inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME,25 mg.kg-1.day-1) in drinking water and swimming (1 hour/day, 5 days/week) to create hypertension and/or exercise models. Ang-(1-7)-induced dilation responses of aortas were examined in the presence/absence of NOS, cyclooxygenase (COX), and endothelium-derived hyperpolarizing factor (EDHF) inhibitors. To assess the roles of the Mas receptor (MasR) and the Angiotensin II-Type2 receptor (AT2R) in Ang-(1-7)-induced vasodilation, the MasR antagonists A-779 and D-Pro⁷-Ang-(1-7), and AT2R inhibitor PD123319 were used.
Results: Swimming exercise improved Ang-(1-7)-mediated vasodilation responses in the E but not in the HE group. Inhibition of NOS and EDHF pathways diminished Ang-(1-7)-induced vasodilation responses in all groups. While vasodilator responses were attenuated only by D-Pro⁷-Ang-(1-7) in the C group, both MasR antagonists reduced these responses in the E and HE groups. In the H group, Ang-(1-7)-induced vasodilation was decreased in the presence of both D-Pro⁷-Ang-(1-7) and PD123319.
Conclusion: Although swimming exercise did not significantly alter Ang-(1-7)-mediated vasodilatory responses in the NOS-inhibited hypertensive model, an enhanced involvement of MasR receptors was observed in the vasodilation responses of the exercise groups, particularly through NOS and EDHF pathways.

Keywords

Angiotensin-(1-7) , Exercise , Hypertension , L-NAME , Swimming

Project Number

Akdeniz University Scientific Research Projects Coordination Unit: TDK-2018-4057

Nitrik oksit sentaz inhibisyonu ile oluşturulan hipertansiyon modelinde yüzme egzersizinin anjiyotensin-(1–7) aracılı aortik gevşeme yanıtlarına etkisi

Abstract

Amaç: Düzenli egzersizin, yüksek kan basıncını düşürücü etkilerinden biri de vasküler tonusu düzenlemesidir. Renin-anjiyotensin sistemi (RAS)’nin üyesi olan Angiotensin-(1-7) [Ang-(1-7)], antihipertansif ve vazodilatör etkilere sahiptir. Bu çalışmayla nitrik oksit sentaz (NOS) inhibisyonuyla oluşturulan hipertansiyon modelinde; düzenli egzersizin kan basıncını düşürücü etkisinde, Ang (1-7) aracılı gevşeme yanıtlarının aracılık edip etmediği araştırılmıştır.
Gereç ve Yöntem: 8 haftalık erkek Wistar sıçanlar kullanıldı. Sıçanlar; Kontrol (K), Hipertansiyon (H), Egzersiz (E) ve Hipertansiyon+Egzersiz (HE) olmak üzere 4 gruba ayrıldı. Hipertansiyon ve/veya egzersiz modeli oluşturmak için sıçanlara; içme suyunda NOS inhibitorü, Nω-nitro-L-arginine methyl ester (L-NAME,25 mg.kg-1.gün-1) ve egzersiz gruplarına 6 hafta boyunca yüzme egzersizi (1 saat/gün,5 gün/hafta) uygulandı. Deneylerin sonunda NOS, siklooksijenaz (COX) ve endotel kaynaklı hiperpolarize edici faktör (EDHF) inhibitörlerinin varlığında/yokluğunda; sıçan aortlarının Ang-(1-7)-aracılı gevşeme yanıtları incelendi. Ang-(1-7)-aracılı gevşeme yanıtlarına Mas reseptör (MasR) ve Anjiyotensin II-Tip 2 (AT2R) reseptörlerinin katkılarını değerlendirmek amacıyla; MasR antagonistleri olarak A-779 ve D-Pro⁷-Ang-(1-7), AT2 reseptör antagonisti olarak PD123,319 kullanıldı.
Bulgular: Yüzme egzersizi; E grubunda Ang-(1-7)-aracılı damar gevşeme yanıtlarında artışa neden olurken, HE grubunda böyle bir etki izlenmedi. NOS ve EDHF yolaklarının inhibisyonu tüm grupların Ang-(1-7)-aracılı gevşeme yanıtlarında azalmaya sebep oldu. K grubunda gevşeme yanıtı yalnızca D-Pro⁷-Ang-(1-7) uygulaması ile azalırken; E ve HE gruplarında gevşeme yanıtlarındaki azalma her iki MasR antagonistinin uygulanmasıyla da gözlendi. H grubundaki Ang-(1-7)-aracılı gevşeme yanıtı, D-Pro⁷-Ang-(1-7) ve PD123,319 antagonistleri varlığında azaldı.
Sonuç: Yüzme egzersizi, NOS-inhibisyonuyla indüklenen hipertansiyon modelinde Ang-(1-7)-aracılı gevşeme yanıtları üzerine istatistiksel olarak anlamlı bir etki oluşturmadı. Ancak egzersiz gruplarından elde edilen gevşeme yanıtlarında MasR’lerinin katkısının daha fazla olduğu ayrıca NOS ve EDHF yolaklarının MasR aracılı gevşeme yanıtlarına aracılık ettiği saptandı.

Keywords

Anjiyotensin-(1-7) , Egzersiz , Hipertansiyon , L-NAME , Yüzme

Project Number

Akdeniz University Scientific Research Projects Coordination Unit: TDK-2018-4057

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