Effect of swimming exercise on angiotensin-(1–7)-mediated aortic dilation responses in the nitric oxide synthase inhibition-induced hypertensive rats
Öz
Purpose: Regular exercise exerts at least some of its effects on lowering high blood pressure by regulating vascular tone. Angiotensin-(1-7) [Ang-(1-7)], a member of the renin–angiotensin system (RAS), has antihypertensive and vasodilator effects. The aim of this study is to investigate whether the blood pressure-lowering effect of regular exercise is mediated by Ang-(1-7)-induced vasodilation in a nitric oxide synthase (NOS) inhibition-induced hypertension model.
Materials and Methods: 8-week-old Wistar male rats were separated into four groups: Control (C), Hypertension (H), Exercise (E), and Hypertension+Exercise (HE). Rats were subjected to the NOS inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME,25 mg.kg-1.day-1) in drinking water and swimming (1 hour/day, 5 days/week) to create hypertension and/or exercise models. Ang-(1-7)-induced dilation responses of aortas were examined in the presence/absence of NOS, cyclooxygenase (COX), and endothelium-derived hyperpolarizing factor (EDHF) inhibitors. To assess the roles of the Mas receptor (MasR) and the Angiotensin II-Type2 receptor (AT2R) in Ang-(1-7)-induced vasodilation, the MasR antagonists A-779 and D-Pro⁷-Ang-(1-7), and AT2R inhibitor PD123319 were used.
Results: Swimming exercise improved Ang-(1-7)-mediated vasodilation responses in the E but not in the HE group. Inhibition of NOS and EDHF pathways diminished Ang-(1-7)-induced vasodilation responses in all groups. While vasodilator responses were attenuated only by D-Pro⁷-Ang-(1-7) in the C group, both MasR antagonists reduced these responses in the E and HE groups. In the H group, Ang-(1-7)-induced vasodilation was decreased in the presence of both D-Pro⁷-Ang-(1-7) and PD123319.
Conclusion: Although swimming exercise did not significantly alter Ang-(1-7)-mediated vasodilatory responses in the NOS-inhibited hypertensive model, an enhanced involvement of MasR receptors was observed in the vasodilation responses of the exercise groups, particularly through NOS and EDHF pathways.
Anahtar Kelimeler
Proje Numarası
Akdeniz University Scientific Research Projects Coordination Unit: TDK-2018-4057
Kaynakça
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