Tumor necrosis factor beta A329G gene polymorphism and the association between patients with acute myocardial infarction
Abstract
Aim: In this study, we aim to evaluate the efficacy of the tumor necrosis factor-beta A329G gene polymorphism in patients admitted to emergency department with chest pain complaint and diagnosed acute myocardial infarction. Material and Method: This study was planned as a prospective, randomized, controlled study and we started the study after the approval of Cukurova University Ethics Committee. Blood samples were taken from the patients who gave permission. Analysis of blood samples were studied in the Biochemistry Laboratory of Mersin University. DNA was extracted by High Pure PCR Template Preparation kit (High Pure PCR Template Preparation kit, Roche Diagnostic, Germany). TNF-β gene polymorphism A329G mutations were determined by using the Light Cycler instrument detection kit (Roche diagnostic, GmbH, Mannheim, Germany) in Light Cycler Real Time PCR. Statistical analysis of data was performed with SPSS 11.5 software package. The statistical significance level of p
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References
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