Effects of herbal mixture on burned rat skin infected with Pseudomonas aeruginosa

Year 2023, Volume: 48 Issue: 2, 472 - 479, 02.07.2023

Serdar Yiğit , Arzu Gezer , Çiğdem Eda Balkan Bozlak , Seda Çelik , Seyit Ali Bingöl

https://doi.org/10.17826/cumj.1242762

Abstract

Purpose: The purpose of this study is to investigate the healing impact of an ointment derived from the Alkanna tinctoria plant upon Pseudomonas aeruginosa-induced infections.
Material and Methods: In this study, 18 male adolescent rats (mean age 6 weeks) weighed an average of 180 g were used. Animals were divided into 3 groups. Group 1: Control group (consisting of burns, no treatment was done), Group 2 (P. aeruginosa): Burn was created and infected with P. aeruginosa, Group 3 (Cream): P. aeruginosa was used to infect the burns area and the herbal mixture was administered twice a day, once in the morning and once in the afternoon. Under anesthesia, the backs of the rats were shaved, and a specially produced steel bar with a diameter of 1*1 cm was immersed in boiling water for 15 seconds before being applied to their backs for 20 seconds. The burned area was subsequently infected with the ATCC Pseudomonas aeruginosa strain, and samples were collected 24 hours later. To detect bacterial growth in this area, the samples were inoculated on blood and EMB (Eosin Methylene Blue) media in a microbiology laboratory. After inoculation, the animals were placed in separate sterile cages and randomly divided into three groups. Once the growth was observed, the tissue and blood samples were harvested from the rats on the 2nd, 7th, 14th, and 21st days.
Results: Epithelial regeneration in this group was more prominent. Vascularization was remarkable on the 2nd day, especially in the group in where we induced a burn and applied the ointment. VEGF levels increased more in the ointment group than in that of others. On the 2nd day of the study, the average bacterial count was 105 in sample of both 2nd and 3rd groups. At the end of the study, while the average of bacterial count was increased in the 2nd group, the average of bacterial count was decreased in the 3rd group.
Conclusion: It was concluded that the ointment obtained from A. tinctorial successfully repaired the epithelial tissue and contributed to the healing of wounds by modifying increasing VEGF in the blood. However, further research is needed before this ointment can be highly recommended for therapeutic usage

Keywords

Alkanna tinctoria, Pseudomonas Aeruginosa, Burn, VEGF, Rat

Pseudomonas aeruginosa ile enfekte olmuş ratlarda oluşan yanıklar üzerine bitkisel karışımların etkileri

Abstract

Amaç: Alkanna tinctoria bitkisinden elde edilen bir merhemin Pseudomonas aeruginosa kaynaklı enfeksiyonlar üzerindeki iyileştirici etkisini araştırmaktır.
Gereç ve Yöntem: Bu çalışmada ortalama 180 gr ağırlığında 18 adet erkek ergen sıçan (ortalama yaş 6 hafta) kullanıldı. Hayvanlar 3 gruba ayrıldı. Grup 1: Kontrol grubu (sadece yanık oluşturuldu, herhangi bir tedavi yapılmadı), Grup 2 (P. aeruginosa): Yanık oluşturuldu ve P. aeruginosa ile enfekte edildi, Grup 3 (Krem): Yanık oluşturularak P. aeruginosa ile enfekte edilerek bitki karışımı sabah akşam uygulandı. Anestezi altında sıçanların sırtları tıraş edildi ve özel olarak üretilmiş 1*1 cm çapında çelik çubuk 15 saniye kaynar suda bekletildikten sonra 20 saniye sırtlarına uygulandı. Yanan alan daha sonra ATCC Pseudomonas aeruginosa suşu ile enfekte edildi ve örnekler 24 saat sonra toplandı. Bu alanda bakteri üremesini saptamak için, numuneler bir mikrobiyoloji laboratuvarında kan ve EMB (Eosin Metilen Mavisi) besiyerine inoküle edildi. Aşılamadan sonra hayvanlar ayrı steril kafeslere yerleştirildi ve rastgele üç gruba ayrıldı. Üreme görüldükten sonra 2., 7., 14. ve 21. günlerde sıçanlardan doku ve kan örnekleri alındı.
Bulgular: Merhem uygulanan grupta epitelyal rejenerasyon daha belirgindi. Özellikle yanık oluşturup pomad uyguladığımız grupta 2. gün damarlanma dikkat çekiciydi. VEGF seviyeleri merhem grubunda diğerlerine göre daha fazla arttı. Çalışmanın 2. gününde, hem 2. hem de 3. grup örneklerinde ortalama bakteri sayısı 105’tir. Çalışmanın sonunda, 2. gruptaki bakteri sayısı ortalaması artarken, 3. gruptaki bakteri sayısı ortalaması azalmıştır.
Sonuç: A. tinctorial'dan elde edilen merhemin epitel dokuyu başarılı bir şekilde onardığı ve kanda artan VEGF'yi modifiye ederek yaraların iyileşmesine katkı sağladığı sonucuna varıldı. Bununla birlikte, bu merhem terapötik kullanım için tavsiye edilmeden önce daha fazla araştırmaya ihtiyaç vardır

Keywords

Burn, Alkanna tinctoria, VEGF, Rat, Pseudomonas Aeruginosa

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