İnsan plasenta kapillerlerinin özgün bir yazılımla 3 boyutlu modellemesi

Year 2023, Volume: 48 Issue: 4, 1380 - 1387, 29.12.2023

Pınar Ayran Fidan

https://doi.org/10.17826/cumj.1365996

Abstract

Amaç: Histolojide 3 boyutlu yapıların zihinde canlandırılması iki boyutlu kesitlerin incelenmesi ile sağlanmaktadır. Ancak yapı işlev bütünlüğünü anlamak her zaman mümkün olmamaktadır. Bu nedenle çalışmamızda laboratuvarımızda geliştirilen ve farklı bilim dallarındaki çalışmalarda kullanılan program ile plasenta terminal villusundaki kapillerlerin 3 boyutlu (3D) modellerinin elde edilmesi planlanmıştır. Programın Histoloji çalışmaları için uygunluğu da değerlendirilecektir.
Gereç ve Yöntem: Çalışmamızda 33 yaşında, sağlıklı gebenin term plasenta dokusundan örnek toplanmıştır. Plastik gömme işlemini takiben bloklardan yarı ince seri kesitler alınmıştır. Alınan seri kesitler Toluidin blue ile boyanarak ışık mikroskopta incelenmiş ve fotoğraflanmıştır. Villöz kapillerlerin rekonstrüksiyonu için seri kesitlerin ışık mikrograflarına üç-boyutlu modelleme prosedürü uygulanmıştır.
Bulgular: Mevcut program ile kesitlerden elde edilen iki boyutlu ışık mikrograflardan 3 boyutlu görüntüler elde edilmiştir. Birbirine paralel uzanan damarların uyumlu bir şekilde dallanarak terminal villusta kapiller ağ yapısı oluşturdukları gözlenmiştir. Ek olarak, multidisipliner olarak kullanılan bu programın histolojik çalışmalarda uygulanması sırasında ortaya çıkan zorluklar da sunulmuştur.
Sonuç: Fetal kan, dallanan kapiller ağ sayesinde terminal villusun her yerine ulaşmaktadır. Bu özellik, vaskülo-sinsityal membranın yüzey alanını artırarak feto-maternal değişimi kolaylaştırmaktadır. Bu özellik üç boyutlu modeller kullanılarak açıkça görselleştirilmiştir. Üç boyutlu modellemeler organların ve dokuların yapısını anlamamıza yardımcı olurlar. Mevcut program serbest erişimli olduğu için çalışmalara ek maliyet yaratmayacaktır ve araştırmacıların ihtiyaçları doğrultusunda programcı tarafından geliştirilmeye açıktır ([e-mail]).

Keywords

kapiller, 3 boyutlu modelleme, plasenta, villus

Project Number

KA23-330

Modeling of human placental capillaries by using a unique 3d reconstruction program

Abstract

Purpose: Visualization of the three-dimensional structures is performed by examining two-dimensional sections in histology. It is not always possible to comprehend structure-function integrity. Therefore, it was planned to obtain three-dimensional (3D) models of the capillaries in the placenta terminal villus by using the program developed in the Başkent University’s laboratory and used in studies in different disciplines. The program’s suitability for histology studies will also be assessed.
Materials and Methods: A term placenta tissue sample was obtained from a 33-year-old healthy woman. Following the plastic embedding procedure, semi-thin serial sections were taken from these blocks. The sections were stained using toluidine blue and examined and photographed by using a light microscope. The 3D modeling procedure was conducted on the light micrographs of serial sections in order to reconstruct villous capillaries.
Results: 3D images were obtained from two-dimensional light micrographs by using the same program. It was determined that the vessels running parallel to each other branched harmoniously, forming a capillary network in the terminal villus. Additionally, challenges encountered during the utilization of this program in histological studies are also presented in this study.
Conclusion: Fetal blood reaches every part of the terminal villus through the branching capillary network. This feature would increase the surface area of vasculo-syncytial membrane and facilitate feto-maternal exchange. This can be clearly visualized by using 3D models. Three-dimensional modeling helps to understand the structure of all organs and tissues. Given that the current program is freely accessible, it will not incur additional costs for researchers and is open to further improvement by the programmer ([e-mail]).

Keywords

capillary, 3D modeling, placenta, villus

Ethical Statement

This study was approved by the Başkent University Institutional Review Board (Project No: DA23/330) and supported by the Başkent University Research Fund.

Project Number

KA23-330

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