An investigation on the cytogenotoxic effects of a spurge species (Euphorbia grisophylla) with partial ethnomedical use in Turkey

Abstract

The effects of the aqueous extract obtained from the aerial parts of Euphorbia grisophylla M.L.S.Khan, a herbaceous member of the Euphorbiaceae family, which is common in other parts of the world outside the Antarctic continent, on genotoxic, cytotoxic, and oxidative parameters were investigated. Experiments were carried out under short-term and in vitro conditions with sublethal concentrations of the test substance extract. According to the first results, the plant extract did not significantly affect the oxidative stress indicators in cell culture. Similarly, it increased the micronucleus ratio in lymphocytes insignificantly, but the concentration-effect relationship is significant in the 24-hour treatment. Again, the test substance did not induce an evident reversion-type mutation in the Ames test. However, the percentage of damaged cells in the Comet test and the genetic damage index increased significantly except for the lowest concentration. The cytotoxic effect observed in the context of the same pattern with these findings is remarkable. In conclusion, the slightly clastogenic and cytotoxic effect of Euphorbia grisophylla aqueous extract, not based on the oxidative attack, may provide a new projection for further research, like cancer therapy.

Keywords

• Euphorbia grisophylla
• Cytogenotoxicity
• Micronucleus (MN) test
• Ames/Salmonella test
• Comet assay

References

1. [2] WHO., (2022). Traditional, Complementary and Integrative Medicine. https://www.who.int/health-topics/traditional-complementary-and-integrative-medicine#tab=tab_1. [accessed June 8, 2022].
2. [3] WHO., (2002). Traditional Medicine Strategy (2002–2005). WHO/EDM/TRM/2002.1. Geneva, Switzerland: World Health Organization. https://apps.who.int/iris/bitstream/handle/10665/67163/WHO_EDM_TRM_2002.1_eng.pdf?sequence=1&isAllowed=y. [accessed June 9, 2022].
3. [4] Veeresham, C., (2012). Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Tech-nology & Research. 3(4): 200. doi: 10.4103/2231-4040.104709.
4. [5] Newman, D.J., Cragg, G.M., (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of Natural Products. 75(3): 311–35. doi: 10.1021/NP200906S.
5. [6] Fabricant, D.S., Farnsworth, N.R., (2001). The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives. 109 Suppl 1(Suppl 1): 69–75. doi: 10.1289/EHP.01109S169.
6. [7] Gillespie, L.J., Armbruster, W.S., (1997). A Contribution to the Guianan Flora: Dalechampia, Haemutostemon, Omp ha lea, Peru, Plukene tia, and Tragia (Euphorbiaceae) with Notes on Subfamily Acalyphoideae. Washington: Smithsonian Institution Press. [8] Salehi, B., Iriti, M., Vitalini, S., Antolak, H., Pawlikowska, E., Kręgiel, D., et al., (2019). Euphorbia-Derived Natural Products with Potential for Use in Health Maintenance. Biomolecules 2019, Vol. 9, Page 337. 9(8): 337. doi: 10.3390/BIOM9080337.