Mine Oluşum Genlerindeki Genetik Polimorfizmler ve Çevresel Faktörler ile Çürük Deneyimi Arasındaki İlişki

Year 2025, Volume: 35 Issue: 4, 282 - 288, 21.10.2025

Büşra Özdemir , Nurcan Özakar , Hasan Dogan

https://doi.org/10.17567/currresdentsci.1485658

Abstract

Amaç: Çürüğün etiyolojisi hem çevresel hem de konakçı faktörleri içermektedir. Bu çalışma, mine oluşum genleri (AMELX, ENAM ve AMBN), çevresel faktörler ve bunların birlikte etkisinin yetişkinlerde çürük deneyimi üzerindeki etkisini değerlendirmeyi amaçlamaktadır.
Yöntemler: 20-65 yaş arası 114 yetişkinin dahil edildiği bir vaka kontrol çalışması yürütüldü. Ağız hijyeni alışkanlıklarına ilişkin veriler hasta bildirimi yoluyla elde edildi. Çürük, plak ve tükürük ile ilgili veriler klinik muayene yoluyla elde edildi. Tükürükten genomik DNA izole edildi. Hastalar çürük deneyimine göre iki gruba ayrıldı. Çevresel faktörler (yaş; plak miktarı; diş fırçalama süresi; şekerli içecek ve yiyecek tüketimi; florürlü diş macunu, florürlü gargara ve diş ipi kullanımı; Streptococcus mutans ve Lactobacillus sayıları) ve konakçı faktörler (tükürük akış hızı, pH, tamponlama kapasitesi ve AMELX, ENAM ve AMBN) incelendi. Genotip dağılımlarını değerlendirmek için gerçek zamanlı PCR kullanıldı. Veriler ki-kare testi ve lojistik regresyon kullanılarak SPSS ile analiz edildi.
Bulgular: Türkiye'den seçilen bireylerde AMELX, ENAM veya AMBN alel ve genotip frekansları arasında gruplar arasında istatistiksel olarak anlamlı bir fark gözlenmedi. Gruplar arasında tükürük akış hızı veya şekerli yiyecek ve içecek tüketimi açısından da anlamlı bir ilişki bulunmadı. Diğer tüm faktörlerin yüksek çürük deneyimi ile ilişkili olduğu tespit edildi.
Sonuç: Çevresel faktörler ve genotipler birlikte çok değişkenli regresyon analizi ile değerlendirildiğinde; artan yaş, yüksek plak seviyeleri ve florürlü gargara kullanımının olmamasının yüksek çürük deneyim olasılığını artırdığı belirlendi.
Anahtar Kelimeler: AMELX, ENAM, AMBN, Genetik Polimorfizm, Çürük deneyimi.

Keywords

AMELX , ENAM , AMBN , Genetik Polimorfizm , Çürük deneyimi.

Project Number

(TDH-2019-7313).

Association between Genetic Polymorphisms in Enamel Formation Genes and Environmental Factors with Caries Experience

Abstract

Objective: The etiology of dental caries involves both environmental and host-related factors. This study aims to evaluate the effect of enamel formation genes (AMELX, ENAM, and AMBN), environmental factors, and their combined influence on caries experience in adults..
Methods: A Case-Control Study was conducted including 114 adults aged 20-65 years. Data regarding oral hygiene habits were obtained through patient declaration. Data regarding caries, plaque and saliva were obtained through clinical examination. Genomic DNA was isolated from saliva. Patients were divided into two groups according to caries experience. Environmental factors (age; amount of plaque; tooth brushing duration; consumption of sugary drinks and foods; use of fluoride toothpaste, fluoride mouthwash and dental floss; Streptococcus mutans and Lactobacillus counts) and host factors (saliva flow rat, pH, buffering capacity and AMELX, ENAM and AMBN) were examined. Real-time PCR was used to evaluate genotype distributions. Data were analyzed using chi-square test and logistic regression with SPSS.
Results: No statistically significant differences were observed in AMELX, ENAM or AMBN Allele and genotype frequencies between groups in selected subjects in Turkey. There was no significant association between the groups with regard to salivary flow rate or consumption of sugary drinks and food. All other factors were found to be associated with high caries experience.
Conclusion: When environmental factors and genotypes were evaluated together using multivariate regression analysis, It was determined that increased age, high plaque levels, and the absence of fluoride mouthwash use increased the likelihood of high caries experience.
Keywords: AMELX, ENAM, AMBN, Genetic Polymorphism, Caries experience

Keywords

AMELX , ; ENAM , AMBN , Genetic Polymorphism , Caries experience.

Ethical Statement

The study protocol was approved by the decision of the Ataturk University Faculty of Medicine Clinical Research Ethics Committee (Number B.30.2.ATA.0.01.00/38). Informed consent forms were obtained in writing from each participant participating in the study.

Supporting Institution

The study was supported by the Ataturk University Scientific Research Projects Foundation (TDH-2019-7313).

Project Number

(TDH-2019-7313).

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