Bioactive Compounds of Myrtle Berrys’ Ultrasound-Assisted Extraction Conditions Optimized by Response Surface Methodology

Abstract

Phytochemicals, such as phenolic compounds, are of great interest due to their health beneficial antioxidant properties and possible protection against inflammation, cardiovascular diseases and certain types of cancer. The bioactive compounds in myrtle berries have shown a potentially positive effect on human health. In this study, bioactive compounds of myrtle berries were optimized by using ultrasound-assisted extraction (USE) technique. The optimal extraction conditions in term of total phenolic content (TPC) and total antioxidant activity (AA) were determined using Response Surface Methodology (RSM) Box–Behnken design. The influence of extraction parameters including, ultrasonication power, sonication time and solid-solvent ratio on TPC and AA were modeled by using a second-order regression equation. The highest desirability was observed with an ultrasonic power of 70, a solid/solvent ratio of 7 %, and an extraction time of 15 min. Sonication power and solid-solvent ratio were the significant parameters for the extraction process (p<0.05). Under these optimized conditions, the predicted value for TPC and DPPH radical scavenging activity were 1.07 g GAE /L extract, and 96.41 %, whereas the observed experimental values were found as 1.164 g GAE /L extract and 100.34 %, respectively. These findings show that extraction of bioactive compounds from myrtle berries using USE technique consumes less solvent and time.

Murt Meyvesinin Biyoaktif Özelliklerinin Ultrases Destekli Ekstraksiyon Yöntemi ile Optimizasyonu

Abstract

Fenolik bileşikler gibi bazı fitokimyasallar, antioksidan özellikleri nedeni ile iltihaplanma, kalp damar hastalıkları ve belirli kanser türlerine karşı olası koruma sağladıklarından büyük ilgi görmektedir. Murt meyvesi (Myrtus communis L.) içerdiği biyoaktif bileşiklerden dolayı insan sağlığı üzerinde potansiyel pozitif etki göstermektedir. Bu çalışmada, murt meyvesinin biyoaktif bileşikleri ultrasonik-destekli ekstraksiyon (USE) yöntemi ile optimize edilmiştir. Murt meyvesi ekstraktlarının toplam fenolik madde içeriği (TFM) ve antioksidan aktivite (AA) açısından optimal ekstraksiyon koşulları, Yanıt Yüzey Yöntemi (RSM) Box-Behnken deney tasarımı kullanılarak belirlenmiştir. Ultrasonikasyon gücü, ultrasonikasyon süresi ve katı-sıvı oranı gibi ekstraksiyon parametrelerinin TFM ve AA üzerindeki etkisi, ikinci dereceden bir regresyon denklemi kullanılarak modellenmiştir. Optimum USE koşulları 70 W sonikasyon gücü, 15 dakika sonikasyon süresi ve % 7 katı-sıvı oranında elde edilmiştir. Sonikasyon gücü ve katı-sıvı oranı TFM ve AA ekstraksiyon işleminde istatistiksel olarak önemli (p<0.05) bulunmuştur. Optimum koşullar altında TFM ve AA sırası ile 1.164 g GAE/L ve % 100.34 olarak tahminlenirken, bu değerler deneysel olarak 1.07 g GAE/L ve % 96.41 olarak bulunmuştur. Elde edilen sonuçlar, deneysel tasarım ile murt meyvesinden biyoaktif bileşiklerin USE yöntemi ile ekstraksiyonunun zaman ve çözgen açısından fayda sağladığını ortaya koymaktadır.

Keywords

• murt
• biyoaktif bileşikler
• ultrases-destekli ekstraksiyon
• optimizasyon

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