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Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri

Year 2025, Volume: 40 Issue: 2, 346 - 355, 30.12.2025

Abstract

Bu çalışma 6 farklı yumrulu kanyaş (Phalaris aquatica L.) genotipinin (Pa-5, Pa-9, Pa-15, Pa-17, Surocco ve Sirosa) alkaloid içeriğini belirlemek ve kurutma işleminin bu bileşikler üzerindeki etkilerini incelemek amacıyla yürütülmüştür. Yaş ve kuru örneklerde HPLC cihazı kullanılarak Gramine, Hordenine, 5-methoxy-tryptamine ve β-karbolin bileşikleri belirlenmiştir. En yüksek toplam alkaloid içeriği Sirosa genotipinin yaş, en düşük ise Pa-17 genotipinin kuru örneğinde saptanmıştır. Kurutma işlemi çoğu genotipte alkaloidleri düşürürken, bazı örneklerde artış gözlenmiştir. Elde edilen bulgular, kaliteli kaba yem üretimi için genotipe özgü kurutma protokollerinin belirlenmesinin ve düşük alkaloid içeriğine sahip genotiplerin seçiminin önemini göstermektedir.

References

  • Açıkgöz, E., Hatipoğlu, R., Altınok, S., Sancak, C., Tan, A., Uraz, D. (2005). Yem bitkileri üretimi ve sorunları. Türkiye Ziraat Mühendisliği VI. Teknik Kongresi, 503(518), 3-7.
  • Akman, N., Aksoy, F., Şahin, O., Kaya, Ç.Y., Erdoğdu, G. (2007). Cumhuriyetimizin 100. Yılında Türkiye’nin Hayvansal Üretimi. Türkiye Damızlık Sığır Yetiştiriciliği Birliği Yayınları No: 4, 116 s
  • Alçiçek, A., Kılıç, A., Ayhan, V., Özdoğan, M. (2010). Türkiye’de kaba yem üretimi ve sorunları. Ziraat Mühendisleri Odası Dergisi.
  • Alden, R., Hackney, B., Weston, L. A., Quinn, J. C. (2014). Phalaris toxicoses in Australian livestock production systems: prevalence, aetiology and toxicology. Journal of Toxins, 1(1), 1-7.
  • Anonim, 2004. :https://www.tarimorman.gov.tr/BUGEM
  • Ball, D. M., Hoveland, C. S. (1978). Alkaloid Levels in Phalaris aquatica L. as Affected by Environment 1. Agronomy Journal, 70(6), 977-981.
  • Başaran, F., Akçin, Z. T. A. (2022). Sıcaklık Faktörünün Bitkiler Üzerindeki Etkileri ve Yüksek Sıcaklık Stresi. Bahçe, 51(2), 139-147.
  • Bourke, C. A., Carrigan, M. J., Dixon, R. J. (1988). Experimental evidence that tryptamine alkaloids do not cause Phalaris aquatica sudden death syndrome in sheep. Australian Veterinary Journal, 65(7), 218-220.
  • Chand, S., Indu, Singhal, R. K., Govindasamy, P. (2022). Agronomical and breeding approaches to improve the nutritional status of forage crops for better livestock productivity. Grass and Forage Science, 77(1), 11-32.
  • Culvenor, R. A., Reed, K. F. M., McDonald, S. E. (2005). Comparative levels of dimethyltryptamine-and tyramine-related alkaloid toxins in Australian cultivars and some wild populations of Phalaris aquatica. Australian Journal of Agricultural Research, 56(12), 1395-1403.
  • Duynisveld, G. W., Slominski, B. A., Wittenberg, K. M., Campbell, L. D. (1990). Alkaloid content of reed canarygrass (Phalaris arundinaceae L.) as determined by gas-liquid chromatography. Canadian Journal of Plant Science, 70(4), 1097-1103.
  • Hill, N. S., Roberts, C. A. (2020). Plant chemistry and antiquality components in forage. Forages: The Science of Grassland Agriculture, 2, 633-658.
  • Hume, D. E., Hickey, M. J., Tapper, B. A. (2007). Degradation of endophyte alkaloids in field-dried cut ryegrass herbage. In Proceedings of the 6th International Symposium on Fungal Endophytes of Grasses. Grassland Research and Practice Series (No. 13, pp. 167-170).
  • Jafarpour, M. H., Pourmohammad, A., Mohammadi, R. (2022). Classification of Phalaris aquatica Genotypes based on Morphological Traits.
  • Kachout, S. S., Youssef, S. B., Khnissi, S., Guenni, K., Zoghlami, A., Ennajah, A., Ghorbel, N., Anchang, J., Hanan, N. P. (2023). Growth responses of the perennial grass, Phalaris aquatica L., to cutting frequency and influence on secondary metabolites and antioxidant activity. Journal of the ASABE, 66(3), 577-587.
  • Kuşvuran, A., Tansı, V., Nazlı, R.İ. (2011). Türkiye’de ve Batı Karadeniz Bölgesi’nde çayır-mera alanları, hayvan varlığı ve yem bitkileri tarımının bugünkü durumu. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 2011(2), 21-32.
  • Li, R. L., Zhang, Q., Liu, J., He, L. Y., Huang, Q. W., Peng, W., Wu, C. J. (2021). Processing methods and mechanisms for alkaloid-rich Chinese herbal medicines: A review. Journal of Integrative Medicine, 19(2), 89-103.
  • Lovett, J. V., Hoult, A. H., Christen, O. (1994). Biologically active secondary metabolites of barley. IV. Hordenine production by different barley lines. Journal of Chemical Ecology, 20, 1945-1954.
  • Mohammadi, R., Pourmohammad, A., Hassanpouraghdam, M. B., Diler, S. (2023). Genetic diversity, heritability, correlation coefficient, and path analysis of forage yield components in Iranian Phalaris aquatica L. genotypes. Turkish Journal of Agriculture and Forestry, 47(3), 335-344.
  • Nazli, R. I., Tansi, V., Öztürk, H. H., Kusvuran, A. (2018). Miscanthus, switchgrass, giant reed, and bulbous canary grass as potential bioenergy crops in a semi-arid Mediterranean environment. Industrial Crops and Products, 125, 9-23.
  • Norton, M. R., Lelièvre, F., Volaire, F. (2012). Summer dormancy in Phalaris aquatica L., the influence of season of sowing and summer moisture regime on two contrasting cultivars. Journal of Agronomy and Crop Science, 198(1), 1-13.
  • Oram, R. N., Ferreira, V., Culvenor, R. A., Hopkins, A. A., Stewart, A. (2009). The first century of Phalaris aquatica L. cultivation and genetic improvement: a review. Crop and Pasture Science, 60(1), 1-15.
  • Özkan, U. (2020). Türkiye yem bitkileri tarımına karşılaştırmalı genel bakış ve değerlendirme. Turkish Journal of Agricultural Engineering Research, 1(1), 29-43.
  • Pan, Q., Mustafa, N. R., Tang, K., Choi, Y. H., Verpoorte, R. (2016) Monoterpenoid indole alkaloids bio-synthesis and its regulation in Catharanthus roseus: a literature review from genes to metabo-lites. Phytochem Rev 15(2), 221-250.
  • Read, E., Reddy, P., Rendell, D., Rochfort, S. (2020). Changes in field concentrations of five phalaris alkaloids and their association with toxicity in pastures of Victoria, Australia. Crop and Pasture Science, 71(4), 389-400 . Reed, K. F. M. (2022). Toxins and phytoestrogens in pasture. Conference: Sowing the Seeds for Success. In Proceedings of the 63rd Annual Conference, Grassland Society of Southern Australia. At: Lardner Park, Victoria, Australia.
  • Riet-Correa, F., Machado, M., Micheloud, J. F. (2023). Plants causing poisoning outbreaks of livestock in South America: A review. Toxicon: X, 17, 100150.
  • Serin, Y., Tan, M. (2001). Yem Bitkileri Kültürüne Giriş. Atatürk Üniversitesi Ziraat Fakültesi Yayınları, No: 206, 217 s.
  • Skerritt, J. H., Guihot, S. L., McDonald, S. E., Culvenor, R. A. (2000). Development of immunoassays for tyramine and tryptamine toxins of Phalaris aquatica L. Journal of Agricultural and Food Chemistry, 48(1), 27-32.
  • Soya, H., Avcıoğlu, R., Geren., H. (2004). Yem Bitkileri. Hasad Yayıncılık, 223 s.
  • Tansı, V. (2009). Kanyaş (Phalaris sp. L.). Yem Bitkileri. Buğdaygil ve Diğer Familyalardan Yem bitkileri, Tarım ve Köy işleri Bakanlığı, Tarımsal Üretim ve Geliştirme Genel Müdürlüğü, İzmir, Cilt III, 843s.
  • TÜİK, 2024. https://www.tuik.gov.tr/
  • Williams, J. D. (1972). Effects of time of day, moisture stress, and frosting on the alkaloid content of Phalaris tuberosa. Australian Journal of Agricultural Research, 23(4), 611-621.
  • Zhou, L., Hopkins, A. A., Huhman, D. V., Sumner, L. W. (2006). Efficient and sensitive method for quantitative analysis of alkaloids in hardinggrass (Phalaris aquatica L.). Journal of Agricultural and Food Chemistry, 54(25), 9287-9291.

Effects of the Drying Process on Alkaloid Content in Different Bulbous Canary Grass (Phalaris aquatica L.) Genotypes

Year 2025, Volume: 40 Issue: 2, 346 - 355, 30.12.2025

Abstract

This study determined the alkaloid content of six tuberous canary grass (Phalaris aquatica L.) genotypes (Pa 5, Pa 9, Pa 15, Pa 17, Surocco, Sirosa) and examined the effects of drying on these compounds. Gramine, hordenine, 5 methoxy tryptamine and β carboline were quantified by HPLC in fresh and dried samples. The highest total alkaloid content was in fresh Sirosa, the lowest in dried Pa 17. While drying reduced alkaloids in most genotypes, some showed increases. These findings underscore the need for genotype specific drying protocols and selecting low alkaloid genotypes for high quality forage production.

References

  • Açıkgöz, E., Hatipoğlu, R., Altınok, S., Sancak, C., Tan, A., Uraz, D. (2005). Yem bitkileri üretimi ve sorunları. Türkiye Ziraat Mühendisliği VI. Teknik Kongresi, 503(518), 3-7.
  • Akman, N., Aksoy, F., Şahin, O., Kaya, Ç.Y., Erdoğdu, G. (2007). Cumhuriyetimizin 100. Yılında Türkiye’nin Hayvansal Üretimi. Türkiye Damızlık Sığır Yetiştiriciliği Birliği Yayınları No: 4, 116 s
  • Alçiçek, A., Kılıç, A., Ayhan, V., Özdoğan, M. (2010). Türkiye’de kaba yem üretimi ve sorunları. Ziraat Mühendisleri Odası Dergisi.
  • Alden, R., Hackney, B., Weston, L. A., Quinn, J. C. (2014). Phalaris toxicoses in Australian livestock production systems: prevalence, aetiology and toxicology. Journal of Toxins, 1(1), 1-7.
  • Anonim, 2004. :https://www.tarimorman.gov.tr/BUGEM
  • Ball, D. M., Hoveland, C. S. (1978). Alkaloid Levels in Phalaris aquatica L. as Affected by Environment 1. Agronomy Journal, 70(6), 977-981.
  • Başaran, F., Akçin, Z. T. A. (2022). Sıcaklık Faktörünün Bitkiler Üzerindeki Etkileri ve Yüksek Sıcaklık Stresi. Bahçe, 51(2), 139-147.
  • Bourke, C. A., Carrigan, M. J., Dixon, R. J. (1988). Experimental evidence that tryptamine alkaloids do not cause Phalaris aquatica sudden death syndrome in sheep. Australian Veterinary Journal, 65(7), 218-220.
  • Chand, S., Indu, Singhal, R. K., Govindasamy, P. (2022). Agronomical and breeding approaches to improve the nutritional status of forage crops for better livestock productivity. Grass and Forage Science, 77(1), 11-32.
  • Culvenor, R. A., Reed, K. F. M., McDonald, S. E. (2005). Comparative levels of dimethyltryptamine-and tyramine-related alkaloid toxins in Australian cultivars and some wild populations of Phalaris aquatica. Australian Journal of Agricultural Research, 56(12), 1395-1403.
  • Duynisveld, G. W., Slominski, B. A., Wittenberg, K. M., Campbell, L. D. (1990). Alkaloid content of reed canarygrass (Phalaris arundinaceae L.) as determined by gas-liquid chromatography. Canadian Journal of Plant Science, 70(4), 1097-1103.
  • Hill, N. S., Roberts, C. A. (2020). Plant chemistry and antiquality components in forage. Forages: The Science of Grassland Agriculture, 2, 633-658.
  • Hume, D. E., Hickey, M. J., Tapper, B. A. (2007). Degradation of endophyte alkaloids in field-dried cut ryegrass herbage. In Proceedings of the 6th International Symposium on Fungal Endophytes of Grasses. Grassland Research and Practice Series (No. 13, pp. 167-170).
  • Jafarpour, M. H., Pourmohammad, A., Mohammadi, R. (2022). Classification of Phalaris aquatica Genotypes based on Morphological Traits.
  • Kachout, S. S., Youssef, S. B., Khnissi, S., Guenni, K., Zoghlami, A., Ennajah, A., Ghorbel, N., Anchang, J., Hanan, N. P. (2023). Growth responses of the perennial grass, Phalaris aquatica L., to cutting frequency and influence on secondary metabolites and antioxidant activity. Journal of the ASABE, 66(3), 577-587.
  • Kuşvuran, A., Tansı, V., Nazlı, R.İ. (2011). Türkiye’de ve Batı Karadeniz Bölgesi’nde çayır-mera alanları, hayvan varlığı ve yem bitkileri tarımının bugünkü durumu. Journal of Agricultural Faculty of Gaziosmanpaşa University (JAFAG), 2011(2), 21-32.
  • Li, R. L., Zhang, Q., Liu, J., He, L. Y., Huang, Q. W., Peng, W., Wu, C. J. (2021). Processing methods and mechanisms for alkaloid-rich Chinese herbal medicines: A review. Journal of Integrative Medicine, 19(2), 89-103.
  • Lovett, J. V., Hoult, A. H., Christen, O. (1994). Biologically active secondary metabolites of barley. IV. Hordenine production by different barley lines. Journal of Chemical Ecology, 20, 1945-1954.
  • Mohammadi, R., Pourmohammad, A., Hassanpouraghdam, M. B., Diler, S. (2023). Genetic diversity, heritability, correlation coefficient, and path analysis of forage yield components in Iranian Phalaris aquatica L. genotypes. Turkish Journal of Agriculture and Forestry, 47(3), 335-344.
  • Nazli, R. I., Tansi, V., Öztürk, H. H., Kusvuran, A. (2018). Miscanthus, switchgrass, giant reed, and bulbous canary grass as potential bioenergy crops in a semi-arid Mediterranean environment. Industrial Crops and Products, 125, 9-23.
  • Norton, M. R., Lelièvre, F., Volaire, F. (2012). Summer dormancy in Phalaris aquatica L., the influence of season of sowing and summer moisture regime on two contrasting cultivars. Journal of Agronomy and Crop Science, 198(1), 1-13.
  • Oram, R. N., Ferreira, V., Culvenor, R. A., Hopkins, A. A., Stewart, A. (2009). The first century of Phalaris aquatica L. cultivation and genetic improvement: a review. Crop and Pasture Science, 60(1), 1-15.
  • Özkan, U. (2020). Türkiye yem bitkileri tarımına karşılaştırmalı genel bakış ve değerlendirme. Turkish Journal of Agricultural Engineering Research, 1(1), 29-43.
  • Pan, Q., Mustafa, N. R., Tang, K., Choi, Y. H., Verpoorte, R. (2016) Monoterpenoid indole alkaloids bio-synthesis and its regulation in Catharanthus roseus: a literature review from genes to metabo-lites. Phytochem Rev 15(2), 221-250.
  • Read, E., Reddy, P., Rendell, D., Rochfort, S. (2020). Changes in field concentrations of five phalaris alkaloids and their association with toxicity in pastures of Victoria, Australia. Crop and Pasture Science, 71(4), 389-400 . Reed, K. F. M. (2022). Toxins and phytoestrogens in pasture. Conference: Sowing the Seeds for Success. In Proceedings of the 63rd Annual Conference, Grassland Society of Southern Australia. At: Lardner Park, Victoria, Australia.
  • Riet-Correa, F., Machado, M., Micheloud, J. F. (2023). Plants causing poisoning outbreaks of livestock in South America: A review. Toxicon: X, 17, 100150.
  • Serin, Y., Tan, M. (2001). Yem Bitkileri Kültürüne Giriş. Atatürk Üniversitesi Ziraat Fakültesi Yayınları, No: 206, 217 s.
  • Skerritt, J. H., Guihot, S. L., McDonald, S. E., Culvenor, R. A. (2000). Development of immunoassays for tyramine and tryptamine toxins of Phalaris aquatica L. Journal of Agricultural and Food Chemistry, 48(1), 27-32.
  • Soya, H., Avcıoğlu, R., Geren., H. (2004). Yem Bitkileri. Hasad Yayıncılık, 223 s.
  • Tansı, V. (2009). Kanyaş (Phalaris sp. L.). Yem Bitkileri. Buğdaygil ve Diğer Familyalardan Yem bitkileri, Tarım ve Köy işleri Bakanlığı, Tarımsal Üretim ve Geliştirme Genel Müdürlüğü, İzmir, Cilt III, 843s.
  • TÜİK, 2024. https://www.tuik.gov.tr/
  • Williams, J. D. (1972). Effects of time of day, moisture stress, and frosting on the alkaloid content of Phalaris tuberosa. Australian Journal of Agricultural Research, 23(4), 611-621.
  • Zhou, L., Hopkins, A. A., Huhman, D. V., Sumner, L. W. (2006). Efficient and sensitive method for quantitative analysis of alkaloids in hardinggrass (Phalaris aquatica L.). Journal of Agricultural and Food Chemistry, 54(25), 9287-9291.
There are 33 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering (Other)
Journal Section Research Article
Authors

Asiye Sena Çavdar 0000-0002-8634-5856

Erdal Ağçam 0000-0002-2677-2020

Recep İrfan Nazlı 0000-0002-6416-6603

Submission Date July 17, 2025
Acceptance Date October 11, 2025
Publication Date December 30, 2025
Published in Issue Year 2025 Volume: 40 Issue: 2

Cite

APA Çavdar, A. S., Ağçam, E., & Nazlı, R. İ. (2025). Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri. Çukurova Tarım Ve Gıda Bilimleri Dergisi, 40(2), 346-355.
AMA Çavdar AS, Ağçam E, Nazlı Rİ. Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri. Çukurova J. Agric. Food. Sciences. December 2025;40(2):346-355.
Chicago Çavdar, Asiye Sena, Erdal Ağçam, and Recep İrfan Nazlı. “Farklı Yumrulu Kanyaş (Phalaris Aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri”. Çukurova Tarım Ve Gıda Bilimleri Dergisi 40, no. 2 (December 2025): 346-55.
EndNote Çavdar AS, Ağçam E, Nazlı Rİ (December 1, 2025) Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri. Çukurova Tarım ve Gıda Bilimleri Dergisi 40 2 346–355.
IEEE A. S. Çavdar, E. Ağçam, and R. İ. Nazlı, “Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri”, Çukurova J. Agric. Food. Sciences, vol. 40, no. 2, pp. 346–355, 2025.
ISNAD Çavdar, Asiye Sena et al. “Farklı Yumrulu Kanyaş (Phalaris Aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri”. Çukurova Tarım ve Gıda Bilimleri Dergisi 40/2 (December2025), 346-355.
JAMA Çavdar AS, Ağçam E, Nazlı Rİ. Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri. Çukurova J. Agric. Food. Sciences. 2025;40:346–355.
MLA Çavdar, Asiye Sena et al. “Farklı Yumrulu Kanyaş (Phalaris Aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri”. Çukurova Tarım Ve Gıda Bilimleri Dergisi, vol. 40, no. 2, 2025, pp. 346-55.
Vancouver Çavdar AS, Ağçam E, Nazlı Rİ. Farklı Yumrulu Kanyaş (Phalaris aquatica L.) Genotiplerinde Kurutma İşleminin Alkaloid İçeriğine Etkileri. Çukurova J. Agric. Food. Sciences. 2025;40(2):346-55.

From January 1, 2016 “Çukurova University Journal of Faculty of Agriculture” continuous its publication life as “Çukurova Journal of Agriculture and Food Sciences”.