Inheritance of resistance to Fusarium oxysporum f.sp. radicis-lycopersici caused root disease in tomato

Year 2012, Volume: 29 Issue: 1, 1 - 8, 01.03.2012

Aylin Kabaş , Hülya İlbi Nedim Mutlu Abdullah Ünlü

Abstract

Fusarium oxysporum f. sp. radicis-lycopersici (FORL) causing crown and root rot brings about significant yield losses in tomato growing areas. In this study, the inheritance of resistance to this disease was investigated. F1, F2, and BC1 from crossing resistant line ‘Fla. 7781’ and susceptible line ‘560’ and parents were inoculated with FORL isolate. 493 F2 plants, 476 BC1 plants, 30 F1 plants, 30 resistant and 30 susceptible plants were used in the test.The results were evaluated according to the χ2 (chi-square) test. Chi-square value was 0.298 (P = 0.585) obtained from F2 segregation population tested for FORL and the hypothesis 3:1/R:S segregation was accepted. Similarly, chi-square value was 1.64 (P = 0.199) obtained from tested BC1 plants and this result did not showed significant deviation from the hypothesis 1:1. The experiment showed that the gene confering resistance, named FORL was single dominant.

Keywords

Tomato; Fusarium oxysporum f.sp.radicis-lycopersici; FORL; Inheritance

Domateste kök ve kök boğazı çürüklüğüne neden olan Fusarium oxysporum f. sp. radicis lycopersici’ye dayanıklılığın kalıtımı

Abstract

Kök ve kök boğazı çürüklüğü etmeni Fusarium oxysporum f. sp. radicis-lycopersici (FORL), domateste önemli verim kaybına sebep olmaktadır. Çalışmada, bu hastalığa dayanıklılığın kalıtımı araştırılmıştır. Dayanıklı Fla. 7781 ile hassas 560 numaralı hattın melezlenmesinden elde edilen F1, F2, BC1 ve ebeveynler, FORL izolatıyla testlenmiştir. Testlemede 493 F2 bitkisi, 476 BC1 bitkisi, 30’ar dayanıklı ve duyarlı ebeveynler ve 30 F1 bitkisi kullanılmıştır. Testleme sonucu X2 (Khi-kare) testine göre değerlendirilmiştir. Test edilen F2 popülasyonunda X2 değeri 0.298 (P=0.585) olup, 3:1 D:H dağılımı hipotezi istatiksel açıdan önemli bir sapma göstermemiştir. Bu sonuca göre dayanıklılık için açılımın 3:1 olduğu belirlenmiştir. Benzer şekilde, BC1 bitkilerinin testleme sonrası X2 değeri 1.64 (P=0.199) bulunmuş ve 1:1 dağılım hipotezinden önemli bir sapma göstermemiştir. Bu bulguların sonucunda dayanıklılığı ifade eden ve FORL olarak isimlendirilen genin tek ve tam dominant bir gen olduğu doğrulanmıştır.

Keywords

Domates; Fusarium oxysporum f. sp. radicis-lycopersici; FORL; Dayanıklılık

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