Development of an Automatic Liquid Dosing System in Microliter Scale

Year 2024, , 512 - 518, 27.09.2024

Serkan Doğanay , Kadri Emre Orgun Ömer Yüce Haydar Barış Öcal Ayberk Kıllı

https://doi.org/10.21205/deufmd.2024267819

Abstract

The goal of this study is to design a novel automatic liquid dosing system for liquid sampling at the microliter level. For this purpose, a mechatronics system is designed to position a syringe at the desired position in the workspace and then drive its piston to inject the liquid to be sampled. Then, an application-specific algorithm is developed to be able to prepare samples in 3x3, 4x4, and 5x5 sample container arrays with a desired volume. The performance tests are conducted for preparing samples with up to three different liquids. The repetitive experiments are performed for 50 and 100 µL sampling volumes. The results indicated that it is possible to dose a single liquid with the highest average deviation of 3.9%. Moreover, it is found that it is possible to prepare a sample with a mixture of three liquids by the highest average deviation from the reference value of around 3.4% when the targeting sampling volume is 250 µL for each liquid.

Keywords

Automatic liquid dosing, liquid sample preparation, mechatronics system design, micro-dosing

Supporting Institution

Scientific and Technological Research Council of Turkey

Project Number

1919B012217833

Thanks

This work was supported by the Scientific and Technological Research Council of Turkey in the scope of 2209A Research Project Support for Undergraduate Students Commission (grant number: 1919B012217833).

Mikrolitre Ölçeğinde Otomatik Sıvı Dozajlama Sistemi Geliştirilmesi

Abstract

Bu çalışmanın amacı, mikrolitre seviyesindeki hacimlerde sıvı numunelerin hazırlanabilmesi için özgün bir otomatik bir sıvı dozajlama sistemi tasarımıdır. Bu kapsamda, belirlenen çalışma alanı içerisinde bir şırınganın pozisyonlandırılması ve içerisindeki sıvının boşaltılması için bir mekatronik sistem tasarımı yapılmıştır. Daha sonra, istenilen hacimlerdeki numunenin 3x3, 4x4 ve 5x5’lik numune kabı dizilimlerinde hazırlanabilmesi için uygulamaya özel bir algoritma geliştirilmiştir. Sistemin performansı, numune hazırlanması sırasında üç adete kadar farklı sıvılar kullanılabilecek şekilde test edilmiştir. 50 ve 100 µL numune hacimleri için tekrarlı deneyler gerçekleştirilmiştir. Elde edilen sonuçlar bir adet sıvının, en fazla %3.9’luk bir ortalama sapma ile dozajlanabileceğini göstermiştir. Ayrıca, testler üç farklı sıvı kullanılarak numune elde etmenin mümkün olduğunu göstermiştir. Bu durumda, her bir sıvıdan 250 µL’lik bir hacim alınarak oluşturulan numunenin, referans olarak verilen hacimden en fazla %3.4’lük bir ortalama sapmaya sahip olduğu gözlemlenmiştir.

Keywords

Otomatik sıvı dozajlama, sıvı numune hazırlama, mekatronik sistem tasarımı, mikro dozajlama

Project Number

1919B012217833

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