17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler

Burak Kaptaner; Güler Ünal

17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler

Abstract

Bu çalışmada, 17α-Etinilestradiol (EE2; 1, 10 ve 100 ng/l) ve 4-n-Nonilfenolün (NP; 10, 60 ve 200 µg/l) nominal konsantrasyonlarına, semistatik koşullarda kronik olarak (32 gün), maruz bırakılan inci kefalinin karaciğer hepatositnde, sitokrom P4501A (CYP1A) enziminin hepatositlerdeki immunohistokimyasal lokalizasyonu, incelendi. Deneysel uygulamalar sonrasında, karaciğer kesitleri immunohistokimya yöntemi ile boyandı ve değerlendirildi. CYP1A immunreaktivitesi, kontrol grubuna ait bireylerde hepatositlerin sitoplazmasında, gözlenmedi. NP uygulanan gruplarda, CYP1A’nın immunohistokimyasal boyanmasında kontrol bireylerine kıyasla, bir farklılık belirlenmedi. EE2 uygulaması sonucunda, hepatositlerin sitoplazmasında nukleus memranından plazma membranına doğru artan ve orta şiddetli olan immunboyanma gözlendi. Elde edilen sonuçlar bu zenoöstrojenlerin, hepatositlerdeki CYP1A immunboyanması üzerine farklı etkiler gösterdiklerini ortaya koymuştur.

Keywords

Chalcalburnus tarichi, CYP1A, karaciğer, hepatosit, 17α-Etinilestradiol, 4-n-Nonilfenol

Supporting Institution

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Project Number

2007-FED-B42

Thanks

Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından desteklenmiştir (Proje No: 2007-FED-B42). Desteğinden dolayı Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı’na teşekkür ederiz.

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Details

Primary Language

Turkish

Subjects

-

Journal Section

Research Article

Authors

Burak Kaptaner ^*
0000-0003-2366-6756
Türkiye

Güler Ünal
0000-0001-5920-6693
Türkiye

Publication Date

September 18, 2020

Submission Date

August 2, 2020

Acceptance Date

September 15, 2020

Published in Issue

Year 2020 Volume: 3 Number: 2

IZ

https://izlik.org/JA47PK64SR

APA

Kaptaner, B., & Ünal, G. (2020). 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi, 3(2), 83-94. https://izlik.org/JA47PK64SR

AMA

1.Kaptaner B, Ünal G. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi. 2020;3(2):83-94. https://izlik.org/JA47PK64SR

Chicago

Kaptaner, Burak, and Güler Ünal. 2020. “17α-Etinilestradiol Ve 4-N-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus Tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi 3 (2): 83-94. https://izlik.org/JA47PK64SR.

EndNote

Kaptaner B, Ünal G (September 1, 2020) 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi 3 2 83–94.

IEEE

[1]B. Kaptaner and G. Ünal, “17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”, Doğu Fen Bilimleri Dergisi, vol. 3, no. 2, pp. 83–94, Sept. 2020, [Online]. Available: https://izlik.org/JA47PK64SR

ISNAD

Kaptaner, Burak - Ünal, Güler. “17α-Etinilestradiol Ve 4-N-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus Tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi 3/2 (September 1, 2020): 83-94. https://izlik.org/JA47PK64SR.

JAMA

1.Kaptaner B, Ünal G. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi. 2020;3:83–94.

MLA

Kaptaner, Burak, and Güler Ünal. “17α-Etinilestradiol Ve 4-N-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus Tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi, vol. 3, no. 2, Sept. 2020, pp. 83-94, https://izlik.org/JA47PK64SR.

Vancouver

1.Burak Kaptaner, Güler Ünal. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi [Internet]. 2020 Sep. 1;3(2):83-94. Available from: https://izlik.org/JA47PK64SR