17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler

Burak Kaptaner; Güler Ünal

17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler

Öz

Bu çalışmada, 17α-Etinilestradiol (EE2; 1, 10 ve 100 ng/l) ve 4-n-Nonilfenolün (NP; 10, 60 ve 200 µg/l) nominal konsantrasyonlarına, semistatik koşullarda kronik olarak (32 gün), maruz bırakılan inci kefalinin karaciğer hepatositnde, sitokrom P4501A (CYP1A) enziminin hepatositlerdeki immunohistokimyasal lokalizasyonu, incelendi. Deneysel uygulamalar sonrasında, karaciğer kesitleri immunohistokimya yöntemi ile boyandı ve değerlendirildi. CYP1A immunreaktivitesi, kontrol grubuna ait bireylerde hepatositlerin sitoplazmasında, gözlenmedi. NP uygulanan gruplarda, CYP1A’nın immunohistokimyasal boyanmasında kontrol bireylerine kıyasla, bir farklılık belirlenmedi. EE2 uygulaması sonucunda, hepatositlerin sitoplazmasında nukleus memranından plazma membranına doğru artan ve orta şiddetli olan immunboyanma gözlendi. Elde edilen sonuçlar bu zenoöstrojenlerin, hepatositlerdeki CYP1A immunboyanması üzerine farklı etkiler gösterdiklerini ortaya koymuştur.

Anahtar Kelimeler

Chalcalburnus tarichi, CYP1A, karaciğer, hepatosit, 17α-Etinilestradiol, 4-n-Nonilfenol

Destekleyen Kurum

Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

2007-FED-B42

Teşekkür

Bu çalışma Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı tarafından desteklenmiştir (Proje No: 2007-FED-B42). Desteğinden dolayı Van Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı’na teşekkür ederiz.

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Burak Kaptaner ^*
0000-0003-2366-6756
Türkiye

Güler Ünal
0000-0001-5920-6693
Türkiye

Yayımlanma Tarihi

18 Eylül 2020

Gönderilme Tarihi

2 Ağustos 2020

Kabul Tarihi

15 Eylül 2020

Yayımlandığı Sayı

Yıl 2020 Cilt: 3 Sayı: 2

IZ

https://izlik.org/JA47PK64SR

APA

Kaptaner, B., & Ünal, G. (2020). 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi, 3(2), 83-94. https://izlik.org/JA47PK64SR

AMA

1.Kaptaner B, Ünal G. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. DOFEBD. 2020;3(2):83-94. https://izlik.org/JA47PK64SR

Chicago

Kaptaner, Burak, ve Güler Ünal. 2020. “17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi 3 (2): 83-94. https://izlik.org/JA47PK64SR.

EndNote

Kaptaner B, Ünal G (01 Eylül 2020) 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. Doğu Fen Bilimleri Dergisi 3 2 83–94.

IEEE

[1]B. Kaptaner ve G. Ünal, “17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”, DOFEBD, c. 3, sy 2, ss. 83–94, Eyl. 2020, [çevrimiçi]. Erişim adresi: https://izlik.org/JA47PK64SR

ISNAD

Kaptaner, Burak - Ünal, Güler. “17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi 3/2 (01 Eylül 2020): 83-94. https://izlik.org/JA47PK64SR.

JAMA

1.Kaptaner B, Ünal G. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. DOFEBD. 2020;3:83–94.

MLA

Kaptaner, Burak, ve Güler Ünal. “17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler”. Doğu Fen Bilimleri Dergisi, c. 3, sy 2, Eylül 2020, ss. 83-94, https://izlik.org/JA47PK64SR.

Vancouver

1.Burak Kaptaner, Güler Ünal. 17α-Etinilestradiol ve 4-n-Nonilfenole Maruz Bırakılan İnci Kefali (Chalcalburnus tarichi, Pallas 1811) (Cyprinidae)’nin Karaciğer Hepatositlerinde Sitokrom P4501A’nın İmmunohistokimyasal Boyanmasındaki Değişimler. DOFEBD [Internet]. 01 Eylül 2020;3(2):83-94. Erişim adresi: https://izlik.org/JA47PK64SR