The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus

İlkay Demircan; Candan Özoğul; Seda Nur Akyol; Mustafa Necmi İlhan; Mustafa Kavutçu; Süreyya Barun; Mustafa Bilge; İbrahim Murat Hirfanoğlu

doi:10.5798/diclemedj.0921.2015.01.0521

The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus

Abstract

Objective: At the present times uterus transplantation is an alternative therapy for women with untreatable uterine-based infertility factors. Before transplantation, the donor organ must stored in some solutions, but they may not adequate for protection. In this study, we investigated the potential protective effects of acetyl L-carnitine, added into histidine-tryptophanketoglutarate (HTK) solution, on rat uterus.

Methods: We divided 24 female Wistar Albino rats into four groups (n=6). Their uterine tissues were stored into four different solutions at different periods at 4oC ; Group 1 HTK, 4 hours; Group 2. HTK + acetyl L- carnitine, 4 hours, Group 3. HTK, 24 hours; Group 4. HTK + acetyl L- carnitine, 24 hours. Then tissues from uterus were used for histological and biochemical examination.

Results: In the study the number of TUNEL positive cells in group 4 was lower than group 3. Biochemically evaluated TBARS and NOS levels were highest in group 3 however CAT level was highest in group 2.

Conclusion: In conclusion, addition of acetyl L-carnitine to HTK solution reversed the histological alterations after 24 hours cold storage on rat uterus.

Keywords

acetyl L-carnitine,uterus,transplantation,infertility

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

İlkay Demircan This is me

Candan Özoğul This is me

Seda Nur Akyol This is me

Mustafa Necmi İlhan This is me

Mustafa Kavutçu This is me

Süreyya Barun This is me

Mustafa Bilge This is me

İbrahim Murat Hirfanoğlu This is me

Publication Date

December 25, 2016

Submission Date

January 11, 2017

Acceptance Date

November 28, 2016

Published in Issue

Year 2016 Volume: 43 Number: 4

DOI

https://doi.org/10.5798/diclemedj.0921.2015.01.0521

IZ

https://izlik.org/JA94KN92US

Cite

RIS / Bibtex

APA

Demircan, İ., Özoğul, C., Akyol, S. N., İlhan, M. N., Kavutçu, M., Barun, S., Bilge, M., & Hirfanoğlu, İ. M. (2016). The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus. Dicle Medical Journal, 43(4), 561-568. https://doi.org/10.5798/diclemedj.0921.2015.01.0521

AMA

1.Demircan İ, Özoğul C, Akyol SN, et al. The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus. Dicle Medical Journal. 2016;43(4):561-568. doi:10.5798/diclemedj.0921.2015.01.0521

Chicago

Demircan, İlkay, Candan Özoğul, Seda Nur Akyol, et al. 2016. “The Protective Effects of Acetyl L - Carnitine Which Added into Histidine - Tryptophan - Ketoglutarate Solution on Donor Uterus”. Dicle Medical Journal 43 (4): 561-68. https://doi.org/10.5798/diclemedj.0921.2015.01.0521.

EndNote

Demircan İ, Özoğul C, Akyol SN, İlhan MN, Kavutçu M, Barun S, Bilge M, Hirfanoğlu İM (December 1, 2016) The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus. Dicle Medical Journal 43 4 561–568.

IEEE

[1]İ. Demircan et al., “The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus”, Dicle Medical Journal, vol. 43, no. 4, pp. 561–568, Dec. 2016, doi: 10.5798/diclemedj.0921.2015.01.0521.

ISNAD

Demircan, İlkay - Özoğul, Candan - Akyol, Seda Nur - İlhan, Mustafa Necmi - Kavutçu, Mustafa - Barun, Süreyya - Bilge, Mustafa - Hirfanoğlu, İbrahim Murat. “The Protective Effects of Acetyl L - Carnitine Which Added into Histidine - Tryptophan - Ketoglutarate Solution on Donor Uterus”. Dicle Medical Journal 43/4 (December 1, 2016): 561-568. https://doi.org/10.5798/diclemedj.0921.2015.01.0521.

JAMA

1.Demircan İ, Özoğul C, Akyol SN, İlhan MN, Kavutçu M, Barun S, Bilge M, Hirfanoğlu İM. The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus. Dicle Medical Journal. 2016;43:561–568.

MLA

Demircan, İlkay, et al. “The Protective Effects of Acetyl L - Carnitine Which Added into Histidine - Tryptophan - Ketoglutarate Solution on Donor Uterus”. Dicle Medical Journal, vol. 43, no. 4, Dec. 2016, pp. 561-8, doi:10.5798/diclemedj.0921.2015.01.0521.

Vancouver

1.İlkay Demircan, Candan Özoğul, Seda Nur Akyol, Mustafa Necmi İlhan, Mustafa Kavutçu, Süreyya Barun, Mustafa Bilge, İbrahim Murat Hirfanoğlu. The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus. Dicle Medical Journal. 2016 Dec. 1;43(4):561-8. doi:10.5798/diclemedj.0921.2015.01.0521