The protective effects of acetyl L - carnitine which added into Histidine - Tryptophan - Ketoglutarate solution on donor uterus

Yıl 2016, , 561 - 568, 25.12.2016

İlkay Demircan Candan Özoğul Seda Nur Akyol Mustafa Necmi İlhan Mustafa Kavutçu Süreyya Barun Mustafa Bilge İbrahim Murat Hirfanoğlu

https://doi.org/10.5798/diclemedj.0921.2015.01.0521

Öz

Objective: At the present times uterus transplantation
is an alternative therapy for women with untreatable uterine-based
infertility factors. Before transplantation, the donor organ must stored
in some solutions, but they may not adequate for protection. In this
study, we investigated the potential protective effects of acetyl
L-carnitine, added into histidine-tryptophanketoglutarate (HTK)
solution, on rat uterus.

Methods: We divided 24 female Wistar Albino rats into
four groups (n=6). Their uterine tissues were stored into four different
solutions at different periods at 4oC ; Group 1 HTK, 4 hours; Group 2.
HTK + acetyl L- carnitine, 4 hours, Group 3. HTK, 24 hours; Group 4. HTK
+ acetyl L- carnitine, 24 hours. Then tissues from uterus were used for
histological and biochemical examination.

Results: In the study the number of TUNEL positive
cells in group 4 was lower than group 3. Biochemically evaluated TBARS
and NOS levels were highest in group 3 however CAT level was highest in
group 2.

Conclusion: In conclusion, addition of acetyl
L-carnitine to HTK solution reversed the histological alterations after
24 hours cold storage on rat uterus.

Anahtar Kelimeler

acetyl L-carnitine, uterus, transplantation, infertility

Kaynakça

• 1. Wranning CA, Marcickiewicz J, Enskog A, et al. Fertility after autologous ovine uterine tubal varian transplantation by vascular anastomosis to the external iliac vessels. Hum Reprod. 2010;25:1973-9.
• 2. Barun S, Özat M, Göngör T, et al. The use of a prostacyclin analog, iloprost, as an adjunct to uterus preservation with histidine tryptophan ketoglutarate (HTK) solution. Transplant Proc. 2011;43:1998-2003.
• 3. Hanafy A, Diaz Garcia C, Olausson M, Brännström M. Uterine transplantation: one human case followed by a decade of experimental research in animal models. Aust N Z J Obstet Gynaecol. 2011;51:199-203
• 4. Racho El Akouri R, Wranning CA, Mölne J, et al. Pregnancy in transplanted mouse uterus after longterm cold ischaemic preservation. Hum Reprod. 2003;18:2024-30.
• 5. Fageeh W, Raffa H, Jabbad H, Marzouki A. Transplantation of the human uterus. Int J Gynaecol Obstet. 2002;76:245-51.
• 6. Wranning CA, El Akouri RR, GrothK, et al. Rejection of the transplanted uterus is suppressed by cyclosporine A in a semi allogeneic mouse model. Hum Reprod. 2007;22:372-9.
• 7. Brännström M, Wranning CA, Altchek A. Experimental uterus transplantation. Hum Reprod Update. 2010;16:329-45.
• 8.Erman Akar M, Ozkan O, Aydinuraz B, et al. Clinical pregnancy after uterus transplantation. Fertil Steril. 2013;100:1358-63
• 9. Brännström M, Johannesson L, Bokström H, et al. Livebirth after uterus transplantation. Lancet. 2015;385:607-16.
• 10. Salahuden AK. Cold ischemic injury of transplanted kidneys: new insights from experimental studies. Am J Physiol Renal Physiol 2004;287:181-7.
• 11. Özkısacık S, Yazıcı M, Gürsoy H, et al. Does Dexpantenol Protect the Kidney from Ischemia Reperfusion Injury. Turkish Nephrology, Dialysis and Transplantation Journal. 2011;20:146-9.
• 12. Tufekci O, Gunes D, Ozogul C, et al. Evaluation of the effect of acetyl L-carnitine on experimental cisplatin nephrotoxicity. Chemotherapy 2009;55:451-9.
• 13. Van Ye TM, Roza AM, Pieper GM,et al. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res. 1993;55:553-8.
• 14. Durak I, Kavutcu M, Kaçmaz M, et al. Effects of isoflurane on nitric oxide metabolism and oxidant status of guinea pig ta Anaesthesiol Scand. 2001;45:119–22.
• 15. Aebi H. Catalase. In: Bergmeyer HU, eds. Methods of Enzymatic Analysis, 2nd edn. New York and London, Academic Pres, 1974;673-677.
• 16. Enskog A, Johannesson L, Chai DC, et al. Uterus transplantation in the baboon:methodology and longterm function after auto ransplantation. Hum Reprod. 2010;25:1980-7.
• 17. Sulikowski T, Domanski L, Zietek Z, et al. Effect of preservation solutions UW and EC on the expression of matrix metalloproteinase II and tissue inhibitor of metalloproteinase II genes in rat kidney. Postepy Hig Med Dosw. 2012;66:45-50.
• 18. Hrabalová M, Bachleda P, Lubuská L, et al. Effect of various protective solutions on function after kidney transplantation. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2003;147:197-202.
• 19. Dolińska B, Ostróżka Cieślik A, Caban A, et al. Comparing the effect of Biolasol® and HTK solutions on maintaining proper omeostasis, indicating the kidney storage efficiency prior to transplantation. Ann Transplant. 2012;17:74-8.
• 20. Jassem W, Heaton ND. The role of mitochondria in ischemia/reperfusion injury in organ transplantation. Kidney Int. 2004;66:514-7.
• 21. Lima ML, Fiorelli AI, Vassallo DV, et al. Comparative experimantalstudy of myocardial protection with crystalloid solutions for the heart transplantation. Rev Bras Cir Cardiovasc 2012;27:110-6.
• 22. Bremer J. Carnitine metabolism and functions. Physiol Rev. 1983;63: 1420-80.
• 23. Mutomba MC, Yuan H, Konyavko M, et al. Regulation of the activity of caspases by L-carnitine and palmitoylcarnitine. FEBS Lett. 2000;478:19-25.
• 24. Budziński G, Wiaderkiewicz R, Caban A, et al. Evaluation of apoptosis in the liver preserved by simple hypothermia using histidinetryptophan ketoglutarate and prolactinmodified histidine tryptophan ketoglutarate solution. Transplant Proc. 2011;43:2900-2.
• 25. Akar H, Saraç A, Konuralp C, et al. Comparison of histopathologic effects of carnitine and ascorbic acid on reperfusion injury. Eur J Cardiothorac Surg. 2001;19:500-6.
• 26. Calò LA, Pagnin E, Davis PA, et al. Antioxidant effect of L -carnitine and its short chain esters: relevance for the protection from oxidative stress related cardiovascular damage. Int J Cardiol 2006;107:54-60.
• 27. Kalaiselvi T, Panneerselvam C. Effect of L-carnitine on thestatus of lipid peroxidation and antioxidants in aging rats. Journal of utritional Biochemistry 1998;9:575-81.
• 28. Trocha M, MerwidLąd A, Chlebda E, et al. Influence of simvastatin on oxidoredox status and nitric oxide synthas protein concentration in rat liver subjected to “cold” ıschemia. Adv Clin Exp Med. 2010:19:43-50.