The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue

Cemil Kahraman; Ahmet Alver; Ayşe Şentürk; İmran İnce Akça

doi:10.5798/dicletip.497874

The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue

Abstract

Objective: High-fat foods increase adipose tissue size, and induce obesity. Although carbonic anhydrase III is abundantly found in brown adipose tissue, its function is not fully defined. In this study, we investigated the relationship between carbonic anhydrase III enzyme mRNA expression and malondialdehyde, oxidative stress marker, in brown adipose tissue of rats that were fed high-fat diets. In addition, we investigated potential effect of N-acetylcysteine as an antioxidant in this relationship.

Methods: In our study three experimental groups were formed and each contained 6 rats (control, obese, and antioxidant groups). The experimental groups were fed for a duration of 85 days with high fat diets. In these groups, carbonic anhydrase III mRNA expression, total carbonic anhydrase hydratase activitie, and malondialdehyde levels were measured in brown adipose tissues dissected from rat scapula regions.

Results: According to our findings, carbonic anhydrase III mRNA expression was higher in the obese group than in the control group (p = 0.004), and malondialdehyde levels were lower in the obese group than in the control group (p = 0.03). It was observed that carbonic anhydrase III mRNA expression was higher in the antioxidant group than in the control group (p = 0.006), and malondialdehyde levels were lower in the antioxidant group than in the control group (p = 0.006). In addition, in the obese group carbonic anhydrase III mRNA expression was higher than in the antioxidant group (p=0.01).

Conclusion: In brown adipose tissue of rats that were fed high-fat diets, this study showed that the carbonic anhydrase III mRNA expression increased and the malondialdehyde level decreased.

Keywords

Carbonic anhydrase III,brown adipose tissue,obesity,N-acetylcysteine

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Details

Primary Language

Turkish

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Cemil Kahraman ^*
0000-0002-4494-6063
Türkiye

Ahmet Alver This is me
0000-0002-9617-6689
Türkiye

Ayşe Şentürk
Türkiye

İmran İnce Akça This is me
0000-0003-2232-3444
Türkiye

Publication Date

December 13, 2018

Submission Date

March 25, 2018

Acceptance Date

October 30, 2018

Published in Issue

Year 2018 Volume: 45 Number: 4

DOI

https://doi.org/10.5798/dicletip.497874

IZ

https://izlik.org/JA96EK22GJ

Cite

RIS / Bibtex

APA

Kahraman, C., Alver, A., Şentürk, A., & Akça, İ. İ. (2018). The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue. Dicle Medical Journal, 45(4), 361-368. https://doi.org/10.5798/dicletip.497874

AMA

1.Kahraman C, Alver A, Şentürk A, Akça İİ. The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue. Dicle Medical Journal. 2018;45(4):361-368. doi:10.5798/dicletip.497874

Chicago

Kahraman, Cemil, Ahmet Alver, Ayşe Şentürk, and İmran İnce Akça. 2018. “The Relationship Between Carbonic Anhydrase-III Expression and Oxidative Stress in Brown Adipose Tissue”. Dicle Medical Journal 45 (4): 361-68. https://doi.org/10.5798/dicletip.497874.

EndNote

Kahraman C, Alver A, Şentürk A, Akça İİ (December 1, 2018) The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue. Dicle Medical Journal 45 4 361–368.

IEEE

[1]C. Kahraman, A. Alver, A. Şentürk, and İ. İ. Akça, “The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue”, Dicle Medical Journal, vol. 45, no. 4, pp. 361–368, Dec. 2018, doi: 10.5798/dicletip.497874.

ISNAD

Kahraman, Cemil - Alver, Ahmet - Şentürk, Ayşe - Akça, İmran İnce. “The Relationship Between Carbonic Anhydrase-III Expression and Oxidative Stress in Brown Adipose Tissue”. Dicle Medical Journal 45/4 (December 1, 2018): 361-368. https://doi.org/10.5798/dicletip.497874.

JAMA

1.Kahraman C, Alver A, Şentürk A, Akça İİ. The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue. Dicle Medical Journal. 2018;45:361–368.

MLA

Kahraman, Cemil, et al. “The Relationship Between Carbonic Anhydrase-III Expression and Oxidative Stress in Brown Adipose Tissue”. Dicle Medical Journal, vol. 45, no. 4, Dec. 2018, pp. 361-8, doi:10.5798/dicletip.497874.

Vancouver

1.Cemil Kahraman, Ahmet Alver, Ayşe Şentürk, İmran İnce Akça. The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue. Dicle Medical Journal. 2018 Dec. 1;45(4):361-8. doi:10.5798/dicletip.497874

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