The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue
Öz
Objective:
High-fat foods increase adipose tissue size, and induce obesity. Although
carbonic anhydrase III is abundantly found in brown adipose tissue, its
function is not fully defined. In this study, we investigated the relationship
between carbonic anhydrase III enzyme mRNA expression and malondialdehyde,
oxidative stress marker, in brown adipose tissue of rats that were fed high-fat
diets. In addition, we investigated potential effect of N-acetylcysteine as an
antioxidant in this relationship.
Methods: In
our study three experimental groups were formed and each contained 6 rats
(control, obese, and antioxidant groups). The experimental groups were fed for
a duration of 85 days with high fat diets. In these groups, carbonic anhydrase
III mRNA expression, total carbonic anhydrase hydratase activitie, and
malondialdehyde levels were measured in brown adipose tissues dissected from
rat scapula regions.
Results:
According to our findings, carbonic anhydrase III mRNA expression was higher in
the obese group than in the control group (p = 0.004), and malondialdehyde
levels were lower in the obese group than in the control group (p = 0.03). It
was observed that carbonic anhydrase III mRNA expression was higher in the
antioxidant group than in the control group (p = 0.006), and malondialdehyde
levels were lower in the antioxidant group than in the control group (p =
0.006). In addition, in the obese group carbonic anhydrase III mRNA expression
was higher than in the antioxidant group (p=0.01).
Conclusion: In brown adipose tissue of rats that were fed high-fat diets, this study showed that the carbonic anhydrase III mRNA expression increased and the malondialdehyde level decreased.
Anahtar Kelimeler
Kaynakça
- 1. Nammi S, Koka S, Chinnala KM, Boini KM. Obesity: An overview on its current perspectives and treatment options. Nutr J. 2004; 3: 1-8.
- 2. Basdevant AB, Aron-Wisnewsky J. Obesity: an evolving process. in: Bastard JP, Feve B (eds) Physiology and physiopathology of adipose tissue. Verlag France: Springer, 2013: 231-42.
- 3. Haslam DW, James WP. Obesity. Lancet. 2005; 366: 1197-209.
- 4. Vázquez-Vela MEF, Torres N, Tovar AR. White adipose tissue as endocrine organ and its role in obesity. Arch Med Res. 2008; 39: 715-28.
- 5. Frühbeck G. Overwiev of adipose tissue and its role in obesity and metabolic disorders. in: Kaiping Yang (ed) Adipose tisue protocols, Second ed. New Jersey: Humana press, 2001: 1-22.
- 6. Gesta S, Tseng YH, Kahn CR. Developmental origin of fat: Tracking obesity to its source. Cell. 2007; 131: 242-56.
- 7. Ibrahim MM. Subcutaneous and visceral adipose tissue: Structural and functional differences. Obes Rev. 2010; 11: 11-8.
- 8. Cannon B, Nedergaard J. Brown Adipose Tissue: Function and physiological significance. Physiol Rev. 2004; 84: 277–359.
Ayrıntılar
Birincil Dil
Türkçe
Konular
Sağlık Kurumları Yönetimi
Bölüm
Araştırma Makalesi
Yazarlar
Cemil Kahraman
*
0000-0002-4494-6063
Türkiye
Ahmet Alver
Bu kişi benim
0000-0002-9617-6689
Türkiye
Ayşe Şentürk
Türkiye
İmran İnce Akça
Bu kişi benim
0000-0003-2232-3444
Türkiye
Yayımlanma Tarihi
13 Aralık 2018
Gönderilme Tarihi
25 Mart 2018
Kabul Tarihi
30 Ekim 2018
Yayımlandığı Sayı
Yıl 2018 Cilt: 45 Sayı: 4