Thiol – Disulphide Homeostasis in Polycythemia Vera

Aysun Şentürk Yıkılmaz; Şule Mine Bakanay; Sema Akinci; Senem Maral; Fuad Mustafayev; Murat Alisik; Özcan Erel; İmdat Dilek

doi:10.5798/dicletip.574893

Thiol – Disulphide Homeostasis in Polycythemia Vera

Abstract

Background: Thiol-disulphide homeostasis has vital role in cell signalling mechanisms, regulation of transcription factors and enzymatic activities, signal transduction and regulation of proliferation rate, apoptosis and detoxification and antioxidant protective mechanism.

Objective: This study aims to demonstrate in Polycythemia Vera (PV) patients the thioldisulphide homeostasis which is known to play a role in cell proliferation, apoptosis and various steps of cell cycle.

Design: Descriptive prospective cross-sectional study.

Settings: Yildirim Beyazıt University Hospital Ankara, Turkey between 2016-2018.

Method: Forty-two PV patients and 43 healthy controls were included in the study. Serum total (–SH + –S–S–) and native (–SH) thiol levels were measured in all subjects. The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method. The data obtained from the patient group were compared with the control group.

Main outcome measures: The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method in PV patieint and healthy control group. Results: Both groups were similar in terms of age and gender distribution. Compared with the control group, PV group had significantly higher native thiol, total thiol and nativ/total thiol levels.

Limitation: The generalizability of the study's findings were limited by the small sample size.

Conclusions: In accordance with the nature of the disease, thiol balance in PV patients was in favor of proliferation. Increased total thiol (–SH + –S–S), native thiol (–SH) levels and native thiol/total thiol ratio might be associated with uncontrolled proliferation. The balance of the thiol-disulphide homeostasis shifted to reductive thiol side in the PV. This change can provocate proliferation status of the disease and/or may be secondary to the disease.

Conflict of Interest: No conflict of interest was declared by the authors.

Keywords

Polycythemia Vera,thiol,disulphide bonds

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Aysun Şentürk Yıkılmaz ^* This is me
0000-0001-5281-5955

Şule Mine Bakanay This is me
0000-0003-3941-0368

Sema Akinci This is me
0000-0003-4237-3342

Senem Maral This is me
0000-0003-4766-1861

Fuad Mustafayev This is me

Murat Alisik This is me
0000-0003-0434-3206

Özcan Erel This is me
0000-0002-2996-3236

İmdat Dilek This is me

Publication Date

June 11, 2019

Submission Date

December 13, 2018

Acceptance Date

April 2, 2019

Published in Issue

Year 2019 Volume: 46 Number: 2

DOI

https://doi.org/10.5798/dicletip.574893

IZ

https://izlik.org/JA99YM83MT

Cite

RIS / Bibtex

APA

Şentürk Yıkılmaz, A., Bakanay, Ş. M., Akinci, S., Maral, S., Mustafayev, F., Alisik, M., Erel, Ö., & Dilek, İ. (2019). Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal, 46(2), 315-320. https://doi.org/10.5798/dicletip.574893

AMA

1.Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, et al. Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal. 2019;46(2):315-320. doi:10.5798/dicletip.574893

Chicago

Şentürk Yıkılmaz, Aysun, Şule Mine Bakanay, Sema Akinci, et al. 2019. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal 46 (2): 315-20. https://doi.org/10.5798/dicletip.574893.

EndNote

Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, Maral S, Mustafayev F, Alisik M, Erel Ö, Dilek İ (June 1, 2019) Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal 46 2 315–320.

IEEE

[1]A. Şentürk Yıkılmaz et al., “Thiol – Disulphide Homeostasis in Polycythemia Vera”, Dicle Medical Journal, vol. 46, no. 2, pp. 315–320, June 2019, doi: 10.5798/dicletip.574893.

ISNAD

Şentürk Yıkılmaz, Aysun - Bakanay, Şule Mine - Akinci, Sema - Maral, Senem - Mustafayev, Fuad - Alisik, Murat - Erel, Özcan - Dilek, İmdat. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal 46/2 (June 1, 2019): 315-320. https://doi.org/10.5798/dicletip.574893.

JAMA

1.Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, Maral S, Mustafayev F, Alisik M, Erel Ö, Dilek İ. Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal. 2019;46:315–320.

MLA

Şentürk Yıkılmaz, Aysun, et al. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal, vol. 46, no. 2, June 2019, pp. 315-20, doi:10.5798/dicletip.574893.

Vancouver

1.Aysun Şentürk Yıkılmaz, Şule Mine Bakanay, Sema Akinci, Senem Maral, Fuad Mustafayev, Murat Alisik, Özcan Erel, İmdat Dilek. Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal. 2019 Jun. 1;46(2):315-20. doi:10.5798/dicletip.574893