Thiol – Disulphide Homeostasis in Polycythemia Vera

Aysun Şentürk Yıkılmaz; Şule Mine Bakanay; Sema Akinci; Senem Maral; Fuad Mustafayev; Murat Alisik; Özcan Erel; İmdat Dilek

doi:10.5798/dicletip.574893

Thiol – Disulphide Homeostasis in Polycythemia Vera

Öz

Background: Thiol-disulphide homeostasis has vital role in cell signalling mechanisms, regulation of transcription factors and enzymatic activities, signal transduction and regulation of proliferation rate, apoptosis and detoxification and antioxidant protective mechanism.

Objective: This study aims to demonstrate in Polycythemia Vera (PV) patients the thioldisulphide homeostasis which is known to play a role in cell proliferation, apoptosis and various steps of cell cycle.

Design: Descriptive prospective cross-sectional study.

Settings: Yildirim Beyazıt University Hospital Ankara, Turkey between 2016-2018.

Method: Forty-two PV patients and 43 healthy controls were included in the study. Serum total (–SH + –S–S–) and native (–SH) thiol levels were measured in all subjects. The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method. The data obtained from the patient group were compared with the control group.

Main outcome measures: The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method in PV patieint and healthy control group. Results: Both groups were similar in terms of age and gender distribution. Compared with the control group, PV group had significantly higher native thiol, total thiol and nativ/total thiol levels.

Limitation: The generalizability of the study's findings were limited by the small sample size.

Conclusions: In accordance with the nature of the disease, thiol balance in PV patients was in favor of proliferation. Increased total thiol (–SH + –S–S), native thiol (–SH) levels and native thiol/total thiol ratio might be associated with uncontrolled proliferation. The balance of the thiol-disulphide homeostasis shifted to reductive thiol side in the PV. This change can provocate proliferation status of the disease and/or may be secondary to the disease.

Conflict of Interest: No conflict of interest was declared by the authors.

Anahtar Kelimeler

Polycythemia Vera,thiol,disulphide bonds

Kaynakça

1. Ruben A. Mesa, Murray N. Silverstein, et all. Population-based incidence and survival figures in essential thrombocythemia and agnogenic myeloid metaplasia: an Olmsted County Study, 1976-1995. Am J Hematol. 1999; 61: 10-5.
2. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem. 2014; 47: 326- 32.
3. Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med. 2013; 65: 244-53.
4. Kundi H, Erel Ö, Balun A, et all. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J. 2015; 49: 95-100.
5. Li W, Busu C, Circu ML, Aw TY. Glutathione in cerebral microvascular endothelial biology and pathobiology: implications for brain homeostasis. Int J Cell Biol. 2012: 434971.
6. Wondrak GT. Redox-directed cancer therapeutics: molecular mechanisms and opportunities. Antioxid Redox Signal. 2009; 11: 3013-69.
7. Kundi H, Ates I, Kiziltunc E, et all. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med. 2015; 33: 1567-71.
8. Ateş İ, Ozkayar N, Altay M, et all. Is disulphide/thiol ratio related to blood pressure in masked hypertension? Clin Exp Hypertens. 2016; 38: 150-4

9. Ates I, Kaplan M, Yuksel M, et all. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016; 51: 47-51.
10. Korkmaz V, Kurdoglu Z, Alisik M, et all. Impairment of thiol-disulphide homeostasis in preeclampsia. J Matern Fetal Neonatal Med. 2016; 3: 1-6
11. Bektas H, Vural G, Gumusyayla S et all. Dynamic thiol-disulphide homeostasis in acute ischemic stroke patients. Acta Neurol Belg. 2016; 116: 489-94.
12. Prabhu A, Sarcar B, Kahali S, et all. Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth. Cancer Res. 2014; 74: 787–96.
13. Kirlin WG, Cai J, Thompson SA, et all. Glutathione redox potential in response to differentiation and enzyme inducers. Free Radic Biol Med. 1999; 27: 1208–18.
14. Nkabyo YS, Ziegler TR, Gu LH, et all. Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cells. Am J Physiol Gastrointest Liver Physiol 2002; 283: 1352–9.
15. Michiels J.J.,Berneman Z.,Schroyens W, et all. Changing Concepts of Diagnostic Criteria of Myeloproliferative Disorders and the Molecular Etiology and Classification of Myeloproliferative Neoplasms: From Dameshek 1950 to Vainchenker 2005 and Beyond, Acta Haematologica. 2015; 133: 36-51.
16. Cao HC, Lin J, Qian J, et all. Detection of the JAK2 mutation in myeloproliferative neoplasms by asymmetric PCR with unlabeled probe and high-resolution melt analysis. J Clin Lab Anal. 2011; 25: 300-4. doi: 10.1002/jcla.20474.
17. Andersen CL, Bjørn ME, McMullin MF, et all l. Circulating YKL-40 in patients with essential thrombocythemia and polycythemia vera treated with the novel histone deacetylase inhibitor vorinostat. Leuk Res. 2014; 38: 816-21.
18. Bjørn ME, Andersen CL, Jensen MK, et all. Circulating YKL-40 in myelofibrosis a potential novel biomarker of disease activity and the inflammatory state. Eur J Haematol. 2014; 93: 224-8.
19. Klampfl T, Gisslinger H, Harutyunyan AS, et all. Somatic mutations of calreticulin in myeloproliferative neoplasms. N Engl J Med. 2013; 369: 2379-90.
20. Bruchova H, Yoon D, Agarwal AM, et all. Erythropoiesis in polycythemia vera is hyper-proliferative and has accelerated maturation. Blood Cells Mol Dis. 2009; 43: 81-7.
21. Laubach JP, Fu P, Jiang X, et all. Polycythemia vera erythroid precursors exhibit increased proliferation and apoptosis resistance associated with abnormal RAS and PI3K pathway activation. Exp Hematol. 2009; 37: 1411-22.
22. Blume KG, Paniker NV, Beutler E. Enzymes of glutathione synthesis in patients with myeloproliferative disorders. Clin Chim Acta. 1973; 45: 281- 5.
23. Ates I, Kaplan M, Yuksel M, et all. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016; 1: 47-51.
24. Yıldırım F, Sermetow K, Aycicek A, et all. Increased oxidative stress in preschool children exposed to passive smoking. J Pediatr (Rio J). 2011; 87: 523-8.
25. Ozler S, Oztas E, Erel O, et al. Impact of Gestational Diabetes Mellitus and Maternal Obesity on Cord Blood Dynamic Thiol/Disulfide Homeostasis. Fetal Pediatr Pathol. 2017; 36: 8-15. doi: 10.1080/15513815.2016.1223237.
26. Babaoglu E, Kilic H, Hezer H, et all. Comparison of thiol/disulphide homeostasis parameters in patients with COPD, asthma and ACOS. Eur Rev Med Pharmacol Sci. 2016; 20: 1537-43.
27. Hanikoglu F, Hanikoglu A, Kucuksayan E, et al. Dynamic Thiol/Disulphide Homeostasis Before and After Radical Prostatectomy in Patients with Prostate Cancer. Free Radic Res. 2016; 50: S79-S84
28. Dirican N, Dirican A, Sen O, et al. Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small cell lung cancer? Redox Rep. 2016; 21: 197-203.
29. Guney T, Kanat IF, Alkan A, et al. Assessment of serum thiol/disulfide homeostasis in multiple myeloma patients by a new method. Redox Report. 2017;22: 246- 51. doi: 10.1080/13510002.2016.1180100.
30. Aldemir M, Karaguzel E, Okulu E, et all. Evaluation of oxidative stress status and antioxidant capacity in patients with renal cell carcinoma. Cent European J Urol. 2015; 68: 415-20.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Sağlık Kurumları Yönetimi

Bölüm

Araştırma Makalesi

Yazarlar

Aysun Şentürk Yıkılmaz ^* Bu kişi benim
0000-0001-5281-5955

Şule Mine Bakanay Bu kişi benim
0000-0003-3941-0368

Sema Akinci Bu kişi benim
0000-0003-4237-3342

Senem Maral Bu kişi benim
0000-0003-4766-1861

Fuad Mustafayev Bu kişi benim

Murat Alisik Bu kişi benim
0000-0003-0434-3206

Özcan Erel Bu kişi benim
0000-0002-2996-3236

İmdat Dilek Bu kişi benim

Yayımlanma Tarihi

11 Haziran 2019

Gönderilme Tarihi

13 Aralık 2018

Kabul Tarihi

2 Nisan 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 46 Sayı: 2

DOI

https://doi.org/10.5798/dicletip.574893

IZ

https://izlik.org/JA99YM83MT

Kaynak Göster

RIS / Bibtex

APA

Şentürk Yıkılmaz, A., Bakanay, Ş. M., Akinci, S., Maral, S., Mustafayev, F., Alisik, M., Erel, Ö., & Dilek, İ. (2019). Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal, 46(2), 315-320. https://doi.org/10.5798/dicletip.574893

AMA

1.Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, vd. Thiol – Disulphide Homeostasis in Polycythemia Vera. diclemedj. 2019;46(2):315-320. doi:10.5798/dicletip.574893

Chicago

Şentürk Yıkılmaz, Aysun, Şule Mine Bakanay, Sema Akinci, vd. 2019. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal 46 (2): 315-20. https://doi.org/10.5798/dicletip.574893.

EndNote

Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, Maral S, Mustafayev F, Alisik M, Erel Ö, Dilek İ (01 Haziran 2019) Thiol – Disulphide Homeostasis in Polycythemia Vera. Dicle Medical Journal 46 2 315–320.

IEEE

[1]A. Şentürk Yıkılmaz vd., “Thiol – Disulphide Homeostasis in Polycythemia Vera”, diclemedj, c. 46, sy 2, ss. 315–320, Haz. 2019, doi: 10.5798/dicletip.574893.

ISNAD

Şentürk Yıkılmaz, Aysun - Bakanay, Şule Mine - Akinci, Sema - Maral, Senem - Mustafayev, Fuad - Alisik, Murat - Erel, Özcan - Dilek, İmdat. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal 46/2 (01 Haziran 2019): 315-320. https://doi.org/10.5798/dicletip.574893.

JAMA

1.Şentürk Yıkılmaz A, Bakanay ŞM, Akinci S, Maral S, Mustafayev F, Alisik M, Erel Ö, Dilek İ. Thiol – Disulphide Homeostasis in Polycythemia Vera. diclemedj. 2019;46:315–320.

MLA

Şentürk Yıkılmaz, Aysun, vd. “Thiol – Disulphide Homeostasis in Polycythemia Vera”. Dicle Medical Journal, c. 46, sy 2, Haziran 2019, ss. 315-20, doi:10.5798/dicletip.574893.

Vancouver

1.Aysun Şentürk Yıkılmaz, Şule Mine Bakanay, Sema Akinci, Senem Maral, Fuad Mustafayev, Murat Alisik, Özcan Erel, İmdat Dilek. Thiol – Disulphide Homeostasis in Polycythemia Vera. diclemedj. 01 Haziran 2019;46(2):315-20. doi:10.5798/dicletip.574893