L-Triptofan ve Melatonin’in Tuz Stresi Altında Bazı Serin Iklim Sebze Türlerinin Tohum Çimlenmesine Etkileri

Abstract

Bu çalışma, farklı konsantrasyonlarda L-Triptofan ve Melatonin ön uygulamalarının, tuz stresine maruz bırakılan soğan, pırasa, siyah havuç ve turp tohumlarının çimlenme özellikleri üzerine etkilerini değerlendirmek amacıyla gerçekleştirilmiştir. Örneklere ait tohumlar, 24 saat boyunca, distile su, 50, 100, 150 ppm L-triptofan;  1.5, 3 ve 4.5 µM Melatonin çözeltilerinde karanlıkta ve 20 ° C' de çözeltilerinde bekletilmiştir. Tohumlar petri kaplarına yerleştirilmiş ve filtre kağıtları farklı NaCl konsantrasyonlarında (0, 150, 300 veya 450 mM) su ile nemlendirilmiştir. Deneme, dört tekerrürlü  tesadüf parselleri şeklinde planlanmıştır. Çalışmanın sonunda maksimum çimlenme yüzdesi, çimlenme indeksi, % 50 çimlenmeyi tamamlama süresi ve ortalama çimlenme süresi değerleri ölçülmüştür. Sonuçlar, ön uygulamaların etkilerinin, siyah havuç tohumlarının ortalama çimlenme süresi hariç, tüm türlerde ve değerlendirilen tüm özellikler üzerinde önemli etkilere neden olduğunu göstermiştir. Buna ek olarak, tuzluluk derecesinin 0 mM' den 450 mM' ye yükselmesi, tohumların tüm çimlenme parametrelerini azaltmış ve geciktirmiştir. Genel olarak, tüm melatonin dozları, 300 mM NaCl stres koşulları altında çimlenme oranını ve çimlenme endeksini bir miktar artırmıştır. Genel olarak, tüm melatonin dozlarından elde edilen sonuçlar birbirine yakın olmasına rağmen, artan L-Triptofan dozları bazı durumlarda olumsuz etkilere neden olmuştur.

Keywords

• L-Trytophan,Melatonin,Tuzluluk,Çimlenme

The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress

Abstract

This study was carried out with the objective to evaluate the effects of different concentrations of L-Tryptophan and Melatonin pretreatment on germination behavior of onion, leek, black carrot, and radish seeds exposed to saline stress. Seeds of samples were soaked for 24 hours in distilled water, 50,100, 150 ppm L-tryptophan, and 1.5, 3, and 4.5 µM melatonin solutions at 20° C in darkness. Seeds were placed in Petri dishes and watered to moisten the filter paper with different concentrations of NaCl (0, 150, 300 or 450 mM). The experiment was planned in a completely randomized design with four replications. Maximum germination percentage, germination index, time to complete 50% germination and mean germination time was measured at the end of the study. The results indicated that the effect of pre-treatment was significant on all studied traits in all species except for mean germination time for black carrot seeds. In addition, all germination parameters of seeds were delayed and decreased by salinity increasing from 0 mM to 450 mM NaCl. Overall, all doses of melatonin increased the maximum germination ratio and germination index values slightly under 300 mM NaCl stress conditions. In general, results which obtained from all doses of melatonin were close to each other, whereas increasing doses of L-tryptophan caused a negative effect in some cases. 

Keywords

• L-Tryptophan,Melatonin,Salinity,Germination,Tolerance

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