Investigation of the effect of SA (Salicylic Acid) application on gene expression during leaf senescence in Arabidopsis thaliana

Year 2019, Volume: 7 Issue: 1, 553 - 565, 31.01.2019

Nihal Gören Sağlam , Gül Öz

https://doi.org/10.29130/dubited.503839

Abstract

Leaf senescence is an important developmental process controlled by many internal and external factors. The
effects of internal factors, especially hormones on leaf senescence is very important. Salicylic Acid (SA) is an
important plant growth regulator known to promote senescence and it is involved in plant defense mechanism. In
this study, the effect of SA on leaf senescence was investigated in terms of gene expression. For this purpose,
salicylic acid was applied to the leaves of Arabidopsis thaliana on the 27th, 31st and 35th days and fluorescence
changes were determined daily. In order to determine gene expression, microarray analysis was performed in the
8th leaves harvested 4 hours after the applications. The data obtained reveal the changes of SA in gene
expression during senescence. 

Keywords

Leaf senescence, Gene expression, Salicylic Acid (SA), Arabidopsis thaliana

Arabidopsis thaliana’da yaprak senesensi sırasında SA (Salisilik Asit) uygulamasının gen anlatımı üzerine etkisinin incelenmesi

Abstract

Yaprak senesensi birçok iç ve dış faktör tarafından kontrol edilen önemli bir gelişimsel süreçtir. İç faktörlerden
özellikle hormonların yaprak senesensi üzerine etkisi çok önemlidir. Salisilik Asit (SA) senesensi teşvik ettiği
bilinen ve bitki savunma mekanizmasında görev alan önemli bir bitki büyüme düzenleyicisidir. Bu çalışmada,
SA’nın yaprak senesensi üzerindeki etkisi gen anlatımı açısından incelenmeye çalışılmıştır. Bu amaçla
Arabidopsis thaliana yapraklarına 27., 31. ve 35. günlerde SA uygulaması yapılmış ve günlük floresans
değişimleri takip edilmiştir. Gen anlatımını tespit edebilmek için uygulamalardan 4 saat sonra hasat edilen 8.
yapraklarda mikroarray analizi yapılmıştır. Elde edilen veriler SA’nın senesens sırasında gen anlatımında yaptığı
değişiklikleri ortaya koymaktadır.

Keywords

Yaprak senesensi, Gen anlatımı, Salisilik Asit (SA), Arabidopsis thaliana

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