A Microscopic and Molecular Survey of Giardia duodenalis in Lambs in Siirt, Türkiye

Year 2023, , 71 - 74, 31.12.2023

Özgür Yaşar Çelik , Burçak Aslan Çelik , Adnan Ayan , Özlem Orunç Kılınç , Kerem Ercan , Muhammed Ahmed Selçuk , Özge Oktay Ayan

https://doi.org/10.47027/duvetfd.1292781

Abstract

Giardia duodenalis is a parasite that causes gastrointestinal diseases and is widespread worldwide. This parasite, which causes infections especially in lambs, is highly prevalent in farm animals. In this study, the prevalence and risk factors of G. duodenalis in lambs were investigated. Lamb fecal samples were collected during the study and microscopic and molecular examinations were performed using Nested PCR method. While Giardia sp. cysts were detected in 28 (23.33%) of 120 samples, the DNA of the parasite was detected in 35 (29.17%) of 120 samples by molecular examination. At the end of the study, the prevalence of G. duodenalis in lambs was recorded between 23.33% and 29.17%. The highest prevalence rate among age groups was found in 16-30 days old lambs, while the highest positivity rate among locations was found in Şirvan district and the lowest positivity rate was found in the center. According to gender, the disease prevalence was higher in females than in males. However, there was no statistically significant difference between location, gender and age groups (p>0.05). In conclusion, G. duodenalis infestations are commonly found in lambs in Siirt province. The results of this study emphasize that control measures and hygiene practices should be implemented to prevent and control G. duodenalis infestations in lambs.

Keywords

Giardia duodenalis, Lamb, PCR, Siirt, Turkey

Türkiye'nin Siirt İlindeki Kuzularda Giardia duodenalis'in Mikroskobik ve Moleküler Yöntemlerle Araştırılması

Abstract

Giardia duodenalis, dünya genelinde yaygın olarak görülen ve gastrointestinal hastalıklara neden olan bir parazittir. Özellikle kuzularda enfeksiyonlara neden olan bu parazit, çiftlik hayvanlarında yüksek oranda görülmektedir. Bu çalışmada, kuzularda G. duodenalis yaygınlığı ve risk faktörleri incelenmiştir. Araştırma süresince kuzu dışkı örnekleri toplanmış, mikroskobik ve Nested PCR yöntemi kullanılarak moleküler inceleme yapılmıştır. Toplam 120 örneğin 28 (%23.33)’inde mikroskobik inceleme sonucu Giardia sp. kistleri tespit edilirken, moleküler inceleme sonucu 120 örneğin 35 (%29.17)’inde parazitin DNA’sı tespit edilmiştir. Araştırma sonunda kuzularda G. duodenalis yaygınlığı %23.33 - %29.17 arasında kaydedilmiştir. Yaş grupları arasında en yüksek prevalans oranı 16-30 günlük kuzularda tespit edilirken, lokasyonlar arasında en yüksek pozitiflik oranı Şirvan ilçesinde, en düşük pozitiflik ise Merkezde bulunmuştur. Cinsiyete göre dişilerde erkeklere göre hastalık prevalansının daha yüksek olduğu kaydedilmiştir. Ancak, lokasyon, cinsiyet ve yaş grupları arasında istatistiksel olarak anlamlı bir fark görülmemiştir (p>0.05). Sonuç olarak, G. duodenalis enfestasyonları Siirt ili kuzularında yaygın olarak bulunmaktadır. Bu çalışmanın sonuçları, kuzularda G. duodenalis enfestasyonlarını önlemek ve kontrol etmek için kontrol önlemlerinin ve hijyen uygulamalarının uygulanması gerektiğini vurgulamaktadır.

Keywords

Giardia duodenalis, Kuzu, PZR, Siirt, Türkiye

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