Iron Metabolism and Haematological Changes in Sheep During The Periparturient Period

Abstract

This study sought to evaluate changes in plasma iron (Fe), unsaturated iron-binding capacity (UIBC), and hematocrit (Hct) levels in pregnant ewes before and after parturition. Blood samples were collected 10 days before and 10 days after birth, and concentrations of Fe, UIBC, and Hct were evaluated. The results indicated a substantial reduction in plasma iron levels postpartum (p=0.042) and a considerable elevation in UIBC levels (p=0.004). No statistically significant variation in Hct level was observed between the prepartum and postpartum periods (p=0.531). The reduction in plasma iron levels may be linked to heightened physiological requirements for iron and bleeding associated with parturition. Increased UIBC levels indicate changes in transferrin binding capacity during the postpartum period. Hct levels remained consistent postnatally, signifying the preservation of hematopoietic equilibrium.

Keywords

• Ewes
• iron
• unsaturated iron-binding capacity

Koyunlarda Periparturient Periyotta Demir Metabolizmasi ve Hematolojik Değişiklikler

Öz

Bu çalışma, gebe koyunlarda doğum öncesi ve sonrası plazma demir (Fe), doymamış demir bağlama kapasitesi (UIBC) ve hematokrit (Hct) seviyelerindeki değişimleri değerlendirmek amacıyla gerçekleştirilmiştir. Çalışmada, doğumdan 10 gün önce ve doğumdan 10 gün sonra alınan kan örneklerinde Fe, UIBC ve Hct düzeyleri analiz edilmiştir. Elde edilen bulgular, doğum sonrası dönemde plazma Fe seviyelerinin anlamlı bir şekilde azaldığını (p=0.0425), buna karşılık UIBC seviyelerinin belirgin bir artış gösterdiğini (p=0.0004) ortaya koymuştur. Hematokrit seviyelerinde ise doğum öncesi ve sonrası dönemler arasında istatistiksel olarak anlamlı bir fark bulunmamıştır (p=0.531). Plazma Fe seviyelerindeki azalma, doğum sırasında meydana gelen kan kaybı ve artan fizyolojik demir ihtiyacı ile ilişkilendirilebilir. UIBC seviyelerindeki artış ise doğum sonrası transferrin bağlanma kapasitesindeki değişiklikleri yansıtmaktadır. Hct seviyelerindeki stabil durum, doğum sonrası hematopoetik dengenin korunduğunu göstermektedir.

Anahtar Kelimeler

• Demir
• doymamış demir bağlama kapasitesi
• koyun

References

1. Missohou A, Nguyen TC, Dorchies P et al. (1998). Note on transferrin, hemoglobin types, and packed cell volume in Senegalese trypanotolerant Djallonke sheep. Ann N Y Acad Sci, 849:209–212.
2. Weiss DJ (2010). Iron and copper deficiencies and disorders of iron metabolism. In: Schalm’s Veterinary Hematology. Weiss DJ, Wardrop KJ (eds). 6th ed., Blackwell, Iowa, USA, 167–171.
3. Cihan H, Temizel EM, Yilmaz Z et al. (2016). Serum iron status and its relation with haematological indexes before and after parturition in sheep. Kafkas Univ Vet Fak Derg, 22(5).
4. Morin DE, Garry FB, Weiser MG et al. (1992). Hematologic features of iron deficiency anemia in llamas. Vet Pathol, 29:400–404.
5. Paltrinieri S, Preatoni M, Rossi S (2010). Microcytosis does not predict serum iron concentrations in anaemic dogs. Vet J, 185:341–343.
6. Kozat S, Yüksek N, Göz Y et al. (2006). Serum iron, total iron-binding capacity, unbound iron-binding capacity, transferrin saturation, serum copper, and hematological parameters in pregnant Akkaraman ewes infected with gastrointestinal parasites. Turk J Vet Anim Sci, 30(6):601–604.
7. Burns LM, Titchener RN, Holmes PH (1992). Blood parameters and turnover data in calves infested with lice. Res Vet Sci, 52:62–66.
8. Breymann C (2002). Iron deficiency and anaemia in pregnancy: modern aspects of diagnosis and therapy. Blood Cells Mol Dis, 29:506–516.

9. Grubač S, Cincović M, Radinović M et al. (2024). Influence of frequent phlebothomy on blood iron concentration, haematological, metabolic and endocrine parameters in rams. Acta Vet, 74(1).
10. Barrionuevo M, Alferez MJM, Lopez Aliaga I et al. (2002). Beneficial effect of goat milk on nutritive utilization of iron and copper in malabsorption syndrome. J Dairy Sci, 85:657–664.
11. Suttle NF (2022). Mineral Nutrition of Livestock. 5th ed., CABI Publishing, Wallingford, UK.
12. Matzek LJ, LeMahieu AM, Madde NR et al. (2022). A contemporary analysis of phlebotomy and iatrogenic anaemia development throughout hospitalization in critically ill adults. Anesth Analg, 135(3):501–510.
13. Szklarz M, Gontarz-Nowak K, Matuszewski W et al. (2022). “Ferrocrinology” – iron is an important factor involved in gluco- and lipocrinology. Nutrients, 14:4693.
14. Alonso ML (2000). Arsenic, cadmium, lead, copper and zinc in cattle from Galicia, NW Spain. Sci Total Environ, 246:237–248.
15. Azab ME, Abdel-Maksoud HA (1999). Changes in some hematological and biochemical parameters during prepartum and postpartum periods in female Baladi goats. Small Ruminant Res, 34(1):77–85.
16. Yokus B, Cakir UD (2006). Seasonal and physiological variations in serum chemistry and mineral concentrations in cattle. Biol Trace Elem Res, 109:255–266.
17. Kılınç MA, Rişvanlı A, Şafak T et al. (2022). The relationship of blood asprosin levels and biochemical parameters in pregnant cows. Turk J Nat Sci, 11(4):111–116.
18. AL-Hadty, H. A. H., AL-Badawi, N. M. (2012). Evaluation of specific biochemical values in clinically normal and anemic Awassi sheep. Int J Sci Nature, 3, 688-691.
19. Şafak T, Yılmaz Ö, Rişvanlı A (2023). Investigation of changes in biochemical parameters in some diseases occurring during the transition period in Simmental cows. Isr J Vet Med, 78(1):18–23.
20. Yenilmez K, Arslan S, Kılıç S et al. (2021). The effect of twinship on selected hematological and biochemical parameters in late pregnant ewes. Med Weter, 77(11):541–545.
21. Khalif AT, Al-Thuwaini TM, Al-Shuhaib MBS (2020). Association of litter size with hematology parameters of Awassi Iraqi ewes. J Kerbala Agric Sci, 7(2):20–26.
22. Habibu B, Kawu MU, Makun HJ et al. (2014). Influence of sex, reproductive status and foetal number on erythrocyte osmotic fragility, haematological and physiologic parameters in goats during the hot-dry season. Vet Med Czech, 59:479–490.
23. Sharma A, Kumar P, Singh M et al. (2015). Haemato-biochemical and endocrine profiling of north western Himalayan Gaddi sheep during various physiological/reproductive phases. Open Vet J, 5:103–107.
24. Soliman EB (2014). Effect of physiological status on some hematological and biochemical parameters of Ossimi sheep. Egypt J Sheep Goats Sci, 9:1–10.