Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı

Year 2021, , 83 - 88, 31.12.2021

Fatma Çelenk

https://doi.org/10.47027/duvetfd.913722

Cited By: 3

Abstract

Çalışma hücre iskeletinin yapısına giren vimentin proteininin boğa ve koç testis, epididimis, duktus deferens ve rete testis deki lokalizasyonlarını ortaya koymak amacıyla planlandı. Araştırmada, 8 adet sağlıklı, yetişkin boğa ve koç tan alınan doku örnekleri kullanıldı. İmmunohistokimyasal boyamalar için Strept-ABC boyama metodu uygulandı. Bu çalışmada, boğalarda ve koçlarda incelenen alanlarda vimentin immunoreaktivitelerinin dağılımı’nın farklı olmadığı görüldü. Vimentin immunoreaktivitesi, seminifer tubüllerdeki Sertoli hücrelerinin perinüklear sitoplazmalarında, intertubüler alanlarda Leydig hücrelerinde ve rete testis epitelleri ile kan damarı endotellerinde belirlendi. Sonuç olarak, Sertoli ve Leyding hücreleri ile rete testis epitel hücrelerinin vimentin intermediyer filamanlarını içermesi, boğa ve koç testislerinde bu yapıların mezenşimal kökenli olduğunun belirtisidir. Ayrıca vimentin filamentlerinin leyding hücrelerinde pozitif boyanması mikrotubüllerin hücresel salgı ürünlerinin taşınmasında aktif bir rol oynadığının göstergesidir. Böylelikle vimentin filamentlerinin erkek genital sistemde hücre iskeletine desteklik sağlama, spermatogenezisin olgunlaşması ve korunması gibi önemli rolleri üstlendiği gösterilmiştir.

Keywords

Vimentin, Testis, Epididimis, Duktus Deferens, Rete testis

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