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Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı

Year 2021, , 83 - 88, 31.12.2021
https://doi.org/10.47027/duvetfd.913722

Abstract

Çalışma hücre iskeletinin yapısına giren vimentin proteininin boğa ve koç testis, epididimis, duktus deferens ve rete testis deki lokalizasyonlarını ortaya koymak amacıyla planlandı. Araştırmada, 8 adet sağlıklı, yetişkin boğa ve koç tan alınan doku örnekleri kullanıldı. İmmunohistokimyasal boyamalar için Strept-ABC boyama metodu uygulandı. Bu çalışmada, boğalarda ve koçlarda incelenen alanlarda vimentin immunoreaktivitelerinin dağılımı’nın farklı olmadığı görüldü. Vimentin immunoreaktivitesi, seminifer tubüllerdeki Sertoli hücrelerinin perinüklear sitoplazmalarında, intertubüler alanlarda Leydig hücrelerinde ve rete testis epitelleri ile kan damarı endotellerinde belirlendi. Sonuç olarak, Sertoli ve Leyding hücreleri ile rete testis epitel hücrelerinin vimentin intermediyer filamanlarını içermesi, boğa ve koç testislerinde bu yapıların mezenşimal kökenli olduğunun belirtisidir. Ayrıca vimentin filamentlerinin leyding hücrelerinde pozitif boyanması mikrotubüllerin hücresel salgı ürünlerinin taşınmasında aktif bir rol oynadığının göstergesidir. Böylelikle vimentin filamentlerinin erkek genital sistemde hücre iskeletine desteklik sağlama, spermatogenezisin olgunlaşması ve korunması gibi önemli rolleri üstlendiği gösterilmiştir.

References

  • 1. Lydka M, Kotula-Balak M, Kopera-Sobota I, Tıschner M, Bılınska B (2011). Vimentin expression in testes of Arabian stallions. Equine Vet J. 43(2): 184-189.
  • 2. Matthew D S, Matthew D A, Folmer J S, Zırkın Barry R (2003). Reduced Intratesticular Testosterone Concentration Alters the Polymerization State of the Sertoli Cell Intermediate Filament Cytoskeleton by Degradation. Endocrinology 144(12): 5530–5536.
  • 3. Aslan Ş, Kocamış H, Nazlı Mümtaz, Gülmez N. (2005). Immunohistochemical Distribution of Desmin and Vimentin in the Skin of Zavot Cattle. Turk J Vet Anim Sci 29: 325-329.
  • 4. Sasano H, Nakashima N, Matsuzaki O, Kato H, Aizawa S, Sasano N, Nagura H. (1992). Testicular sex cord-stromal lesions: immunohistochemical analysis of cytokeratin, vimentin and steroidogenic enzymes. Virchows Archiv A Pathol Anat. 421: 163-169.
  • 5. Aire TA, Ozegbe PC, Soley J T, Madekurozwa MC. (2008). Structural and Immunohistochemical Features of the Epididymal Duct Unit of the Ostrich (Struthio camelus). Anat. Histol. Embryol. 37: 296–302.
  • 6. Dinges H P, Zatloukal K, Schmid C, Mair S, Wirnsberger G. Co-expression of cytokeratin and vimentin filaments in rete testis and epididymis. Virchows Archiv A Pathol Anat (1991) 418:119-127.
  • 7. Davidoff MS, Middendorff R, Pusch W. et al. (1999). Sertoli and Leydig cells of the human testis express neurofilament triplet proteins. Histochem Cell Biol. 111:173–187.
  • 8. Beyaz F, Küçük Bayram G, Alan Emel. 2009. Vimentin, Sitokeratin, α-SMA ve Desmin’in Yeni Zellanda Tavşanı Testis ve Epididimisindeki İmmunohistokimyasal Ekspresyonu. Erciyes Üniv Vet Fak Derg 6(2) 111-119.
  • 9. Devkota B, Sasaki M, Takahaski KI, et al. Postnatal Developmental Changes in Immunohistochemical Localization of α-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testis. J. Reprod. Dev. 52: 43-49, 2006.
  • 10. Steger K, SchımmeL M, Wrobel KH. Immunocytochemical Demonstration of Cytoskeletal Proteins in Seminiferous Tubules of Adult Rams and Bulls. Arch. Histol. Cytol., Vol. 57, No. 1 (1994).
  • 11. Devkota B, Sasaki M, Matsui M, Montoya CA, Miyake YI. Alterations in the İmmunohistochemical Localization Patterns of α-Smooth Muscle Actin (SMA) and Vimentin in the Postnatally Developing Bovine Cryptorchid Testis. J.Reprod. Dev. 52: 329-334, 2006.
  • 12. Tung P S, Rosenıor J, Frıtz I B. (1987). Isolation and Culture of Ram Rete Testis Epithelial Cells: Structural and Biochemical Characteristics. Bıology of Reproductıon 36: 1297-1312.
  • 13. Komatsu T, Yamamoto Y, Atoji Y, Tsubota T, Suzuki Y. (1998). Immunohistochemical Demonstration of Cytoskeletal Proteins in the Testis of the Japanese Black Bear, Ursus thibetanus japonicus. Anat. Histol. Embryol. 27: 209-213.
  • 14. Rodrıguez A, Rojas MA, Obregon EB, Urquıeta B. et. al. (1999). Distribution of Keratins, Vimentin, and Actin in the Testis of Two South American Camelids: Vicuna (Vicugna vicugna) and Llama (Lama glama). An Immunohistochemical Study. THE Anatomıcal Record 254: 330–335.
  • 15. Matthew DS, Matthew DA, Folmer JS, Zırkın BR. (2003). Reduced Intratesticular Testosterone Concentration Alters the Polymerization State of the Sertoli Cell Intermediate Filament Cytoskeleton by Degradation of Vimentin. Endocrinology 144(12): 5530–5536.
  • 16. Rıchburg JH, Boekelheıde K. (1996). Mono-(2-ethylhexyl) Phthalate Rapidly Alters both Sertoli Cell Vimentin Filaments and Germ Cell Apoptosis in Young Rat Testes. Toxıcology and Applıed Pharmacology 137: 42–50.
  • 17. Zhang ZH, Hu ZY, SONG XX, Xıao LJ, Zou RJ, Han CS, Lıu YX. (2004). Disrupted expression of intermediate filaments in the testisof rhesus monkey after experimental cryptorchidism. İnternational Journal of Andrology. 27: 234–239.
  • 18. Aumfiller G, Schulze C, Viebahn C. (1992). Intermediate filaments in Sertoli cells. Microsc Res Tech. 20: 50-72.
  • 19. Allard EK, Johnson KJ, Boekelheide K. (1993). Colchicine disrupts the cytoskeleton of rat testis seminiferous epithelium in a stage dependent manner. Biol Reprod. 48: 143-153.
  • 20. Steger K, Wrobel KH. (1994). Immunohistochemical demonstration of cytoskeletal proteins in the ovine testis during postnatal development. Anat Embryol. 189: 521-530.
  • 21. Czernobilsky B, Moll R, Levy R, Franke WW. (1985). Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol. 37: 175-90.
  • 22. Achtsta¨ tter, T., R. Moll, B. Moore, and W. W. Franke. (1985). Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract. J. Histochem Cytochem. 33: 415-426.
  • 23. Kasper, M., and P. Stosiek. (1989). Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood. Cell Tissue Res. 257: 661–664.
  • 24. Wakui, S., M. Furusato, S. Ushigome, and Y. Kano. (1994). Coexpression of different cytokeratins, vimentin and desmin in the rete testis and epididymis of the dog. J. Anat. 184: 147-151.
Year 2021, , 83 - 88, 31.12.2021
https://doi.org/10.47027/duvetfd.913722

Abstract

References

  • 1. Lydka M, Kotula-Balak M, Kopera-Sobota I, Tıschner M, Bılınska B (2011). Vimentin expression in testes of Arabian stallions. Equine Vet J. 43(2): 184-189.
  • 2. Matthew D S, Matthew D A, Folmer J S, Zırkın Barry R (2003). Reduced Intratesticular Testosterone Concentration Alters the Polymerization State of the Sertoli Cell Intermediate Filament Cytoskeleton by Degradation. Endocrinology 144(12): 5530–5536.
  • 3. Aslan Ş, Kocamış H, Nazlı Mümtaz, Gülmez N. (2005). Immunohistochemical Distribution of Desmin and Vimentin in the Skin of Zavot Cattle. Turk J Vet Anim Sci 29: 325-329.
  • 4. Sasano H, Nakashima N, Matsuzaki O, Kato H, Aizawa S, Sasano N, Nagura H. (1992). Testicular sex cord-stromal lesions: immunohistochemical analysis of cytokeratin, vimentin and steroidogenic enzymes. Virchows Archiv A Pathol Anat. 421: 163-169.
  • 5. Aire TA, Ozegbe PC, Soley J T, Madekurozwa MC. (2008). Structural and Immunohistochemical Features of the Epididymal Duct Unit of the Ostrich (Struthio camelus). Anat. Histol. Embryol. 37: 296–302.
  • 6. Dinges H P, Zatloukal K, Schmid C, Mair S, Wirnsberger G. Co-expression of cytokeratin and vimentin filaments in rete testis and epididymis. Virchows Archiv A Pathol Anat (1991) 418:119-127.
  • 7. Davidoff MS, Middendorff R, Pusch W. et al. (1999). Sertoli and Leydig cells of the human testis express neurofilament triplet proteins. Histochem Cell Biol. 111:173–187.
  • 8. Beyaz F, Küçük Bayram G, Alan Emel. 2009. Vimentin, Sitokeratin, α-SMA ve Desmin’in Yeni Zellanda Tavşanı Testis ve Epididimisindeki İmmunohistokimyasal Ekspresyonu. Erciyes Üniv Vet Fak Derg 6(2) 111-119.
  • 9. Devkota B, Sasaki M, Takahaski KI, et al. Postnatal Developmental Changes in Immunohistochemical Localization of α-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testis. J. Reprod. Dev. 52: 43-49, 2006.
  • 10. Steger K, SchımmeL M, Wrobel KH. Immunocytochemical Demonstration of Cytoskeletal Proteins in Seminiferous Tubules of Adult Rams and Bulls. Arch. Histol. Cytol., Vol. 57, No. 1 (1994).
  • 11. Devkota B, Sasaki M, Matsui M, Montoya CA, Miyake YI. Alterations in the İmmunohistochemical Localization Patterns of α-Smooth Muscle Actin (SMA) and Vimentin in the Postnatally Developing Bovine Cryptorchid Testis. J.Reprod. Dev. 52: 329-334, 2006.
  • 12. Tung P S, Rosenıor J, Frıtz I B. (1987). Isolation and Culture of Ram Rete Testis Epithelial Cells: Structural and Biochemical Characteristics. Bıology of Reproductıon 36: 1297-1312.
  • 13. Komatsu T, Yamamoto Y, Atoji Y, Tsubota T, Suzuki Y. (1998). Immunohistochemical Demonstration of Cytoskeletal Proteins in the Testis of the Japanese Black Bear, Ursus thibetanus japonicus. Anat. Histol. Embryol. 27: 209-213.
  • 14. Rodrıguez A, Rojas MA, Obregon EB, Urquıeta B. et. al. (1999). Distribution of Keratins, Vimentin, and Actin in the Testis of Two South American Camelids: Vicuna (Vicugna vicugna) and Llama (Lama glama). An Immunohistochemical Study. THE Anatomıcal Record 254: 330–335.
  • 15. Matthew DS, Matthew DA, Folmer JS, Zırkın BR. (2003). Reduced Intratesticular Testosterone Concentration Alters the Polymerization State of the Sertoli Cell Intermediate Filament Cytoskeleton by Degradation of Vimentin. Endocrinology 144(12): 5530–5536.
  • 16. Rıchburg JH, Boekelheıde K. (1996). Mono-(2-ethylhexyl) Phthalate Rapidly Alters both Sertoli Cell Vimentin Filaments and Germ Cell Apoptosis in Young Rat Testes. Toxıcology and Applıed Pharmacology 137: 42–50.
  • 17. Zhang ZH, Hu ZY, SONG XX, Xıao LJ, Zou RJ, Han CS, Lıu YX. (2004). Disrupted expression of intermediate filaments in the testisof rhesus monkey after experimental cryptorchidism. İnternational Journal of Andrology. 27: 234–239.
  • 18. Aumfiller G, Schulze C, Viebahn C. (1992). Intermediate filaments in Sertoli cells. Microsc Res Tech. 20: 50-72.
  • 19. Allard EK, Johnson KJ, Boekelheide K. (1993). Colchicine disrupts the cytoskeleton of rat testis seminiferous epithelium in a stage dependent manner. Biol Reprod. 48: 143-153.
  • 20. Steger K, Wrobel KH. (1994). Immunohistochemical demonstration of cytoskeletal proteins in the ovine testis during postnatal development. Anat Embryol. 189: 521-530.
  • 21. Czernobilsky B, Moll R, Levy R, Franke WW. (1985). Co-expression of cytokeratin and vimentin filaments in mesothelial, granulosa and rete ovarii cells of the human ovary. Eur J Cell Biol. 37: 175-90.
  • 22. Achtsta¨ tter, T., R. Moll, B. Moore, and W. W. Franke. (1985). Cytokeratin polypeptide patterns of different epithelia of the human male urogenital tract. J. Histochem Cytochem. 33: 415-426.
  • 23. Kasper, M., and P. Stosiek. (1989). Immunohistochemical investigation of different cytokeratins and vimentin in the human epididymis from the fetal period up to adulthood. Cell Tissue Res. 257: 661–664.
  • 24. Wakui, S., M. Furusato, S. Ushigome, and Y. Kano. (1994). Coexpression of different cytokeratins, vimentin and desmin in the rete testis and epididymis of the dog. J. Anat. 184: 147-151.
There are 24 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research
Authors

Fatma Çelenk 0000-0002-9677-8372

Publication Date December 31, 2021
Acceptance Date May 24, 2021
Published in Issue Year 2021

Cite

APA Çelenk, F. (2021). Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 14(2), 83-88. https://doi.org/10.47027/duvetfd.913722
AMA Çelenk F. Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniv Vet Fak Derg. December 2021;14(2):83-88. doi:10.47027/duvetfd.913722
Chicago Çelenk, Fatma. “Yetişkin Boğa Ve Koçların Testis Ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14, no. 2 (December 2021): 83-88. https://doi.org/10.47027/duvetfd.913722.
EndNote Çelenk F (December 1, 2021) Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14 2 83–88.
IEEE F. Çelenk, “Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı”, Dicle Üniv Vet Fak Derg, vol. 14, no. 2, pp. 83–88, 2021, doi: 10.47027/duvetfd.913722.
ISNAD Çelenk, Fatma. “Yetişkin Boğa Ve Koçların Testis Ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14/2 (December 2021), 83-88. https://doi.org/10.47027/duvetfd.913722.
JAMA Çelenk F. Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniv Vet Fak Derg. 2021;14:83–88.
MLA Çelenk, Fatma. “Yetişkin Boğa Ve Koçların Testis Ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 14, no. 2, 2021, pp. 83-88, doi:10.47027/duvetfd.913722.
Vancouver Çelenk F. Yetişkin Boğa ve Koçların Testis ve Epididimal Kanal Ünitesindeki Vimentinin İmmünohistokimyasal Dağılımı. Dicle Üniv Vet Fak Derg. 2021;14(2):83-8.