Antimicrobial Efficiency of Chitosan Coatings Containing Garlic Essential Oil on The Preservation of Rainbow Trout Fillets

Year 2022, Volume: 6 Issue: 2, 160 - 165, 29.12.2022

İlknur Uçak

Abstract

This study objected to evaluate the antimicrobial effects of chitosan coatings enriched with garlic essential oil in the rainbow trout fillets during cold storage. Rainbow trout fillets were prepared with chitosan coating solutions with (GEO) and without (CF) garlic essential oil emulsion. One group left as control (C) without chitosan coating. All samples were stored at refrigeration for 15 days and during the storage period total psychrophilic bacteria, total mesophilic bacteria and total Enterobacteriaceae counts were determined. During the storage period the highest bacteria growth was reported in the control group and the group coated with chitosan solution. At the end of the storage, total psychrophilic bacteria count was found as 5.34, 4.71 and 4.16 log CFU/g in C, CF and GEO groups, respectively. Control group showed the highest total mesophilic bacteria count at 15th day (5.91 log CFU/g), while this value was 5.24 log CFU/g and 4.83 log CFU/g in the CF and GEO, respectively. The group coated with chitosan solutions containing garlic essential oil emulsion showed the lowest Total Enterobacteriaceae count during the storage and remained as 3.82 log CFU/g at the end of the storage. The results showed that the addition of garlic essential oil emulsion in the chitosan solutions as coating material is showed antimicrobial effect in the rainbow trout fillets during refrigerated storage.

Keywords

chitosan, rainbow trout, garlic essential oil, antimicrobial effect

References

• Alsaggaf, M., Moussa, S. H., & Tayel, A. A. 2017. Application of fungal chitosan incorporated with pomegranate peel extract as edible coating for microbiological, chemical and sensorial quality enhancement of Nile tilapia fillets. International Journal of Biological Macromolecules, 99, 499-505.
• Ebadi, Z., Khodanazary, A., Hosseini, S. M., & Zanguee, N. 2019. The shelf life extension of refrigerated Nemipterus japonicus fillets by chitosan coating incorporated with propolis extract. International Journal of Biological Macromolecules, 139, 94-102.
• FDA. Enumeration of Escherichia coli and the coliform bacteria, bacterial analytical manual, 8th ed.; Washington, DC: AOAC International, 1998.
• Frangos, L., Pyrgotou, N., Giatrakou, V., Ntzimani, A., & Savvaidis, I. N. 2010. Combined effects of salting, oregano oil and vacuum-packaging on the shelf-life of refrigerated trout fillets. Food Microbiology, 27(1), 115-121.
• Hosseini, S. F., Rezaei, M., Zandi, M., & Ghavi, F. F. 2016. Effect of fish gelatin coating enriched with oregano essential oil on the quality of refrigerated rainbow trout fillet. Journal of Aquatic Food Product Technology, 25(6), 835-842.
• ICMSF. Microorganisms in foods. Their significance and methods of enumeration, 2nd ed.; London: Univ. Toronto Pres. 1982.
• Jouki, M., Yazdi, F. T., Mortazavi, S. A., Koocheki, A., & Khazaei, N. 2014. Effect of quince seed mucilage edible films incorporated with oregano or thyme essential oil on shelf life extension of refrigerated rainbow trout fillets. International Journal of Food Microbiology, 174, 88-97.
• Keser, İ. & İzci, L. 2020. Gökkuşağı Alabalığı (Oncorhynchus mykiss)’ndan Elde Edilen Balık Köftelerinde Biberiye ve Defne Uçucu Yağlarının Mikrobiyolojik ve Duyusal Kaliteye Etkisi. Acta Aquatica Turcica, 16(1), 13-21.
• Ojagh, S. M., Rezaei, M., Razavi, S. H., & Hosseini, S. M. H. 2010. Effect of chitosan coatings enriched with cinnamon oil on the quality of refrigerated rainbow trout. Food Chemistry, 120(1), 193-198.
• Öz, M. (2018). Effects of garlic (Allium sativum) supplemented fish diet on sensory, chemical and microbiological properties of rainbow trout during storage at -18°C. LWT-Food Science and Technology, 92, 155-160.
• Renur, N. M., Haryadi, Y., Darmawati, E., & Kapelle, E. B. D. 2016. Application of chitosan and chinese lemon extract (Citrus mitis) based edible coating on tuna fillet. International Journal of Food Sciences and Nutrition, 4(2), 29-33.
• Shahbazi, Y., & Shavisi, N. 2018. Chitosan coatings containing Mentha spicata essential oil and zinc oxide nanoparticle for shelf life extension of rainbow trout fillets. Journal of Aquatic Food Product Technology, 27(9), 986-997.
• Ucak, I., Ozogul, Y., & Durmus, M. 2011. The effects of rosemary extract combination with vacuum packing on the quality changes of Atlantic mackerel fish burgers. International Journal of Food Science and Technology, 46, 1157-1163.
• Ucak, I. 2019. Physicochemical and antimicrobial effects of gelatin-based edible films incorporated with garlic peel extract on the rainbow trout fillets. Progress in Nutrition, 21(1), 232-240.
• Ucak, I. 2020. Soğukta depolanan (4±1°C) alabalık burgerlerde nar kabuğu ekstraktının antioksidan ve antimikrobiyal etkilerinin belirlenmesi. Ege Journal of Fisheries and Aquatic Sciences, 37(4), 415-422.
• Ucak, I., Abuibaid, A. K. M., Aldawoud, T. M. S., Galanakis, C. M., & Montesano, D. 2021. Antioxidant and antimicrobial effects of gelatin films incorporated with citrus seed extract on the shelf life of sea bass (Dicentrarchus labrax) fillets. Journal of Food Processing and Preservation, 45(4), e15304.
• Ucak, I., Yerlikaya, P., Khalily, R., & Abuibaid, A. K. M. 2019. Effects of Gelatin Edible Films Containing Onion Peel Extract on the Quality of Rainbow Trout Fillets. Eurasian Journal of Food Science and Technology, 3(2), 40-48.
• Ucak, I., Khalily, R., Abuibaid, A.K.M. & Abidemi Ogunkalu, O. 2018. Maintaining the quality of rainbow trout (Oncorhynchus mykiss) fillets by treatment of red onion peel extract during refrigerated storage. Progress in Nutrition, 20(4), 672-678.
• Ucak, I., & Afreen, M. 2022. Effect of Chitosan Coating Enriched with Peppermint Essential Oil Emulsion on the Microbiological Quality of Fish Meatballs. Eurasian Journal of Food Science and Technology, 6(1), 60-68.